Even though there are several drawbacks when attempting to accurately performing MS2, such as requiring a stem-loop in order to function. MS2 is still an efficient method of viewing the mechanisms of mRNA localization in living cells.
Boireau S, Maiuri P, Basyuk E, de la Mata M, Knezevich A, Pradet-Balade B, et al. The transcriptional cycle of HIV-1 in real-time and live cells. J. Cell Biol 2007; 179:291–304.
This article is interested in looking at the activity of RNA polymerase II by using the transcription of the human immunodeficiency virus type 1 (HIV-1) to examine the synthesis of mRNA at specific transcription sites. HIV-1 works by transcribing high levels of the virus while only allowing the infected cell to transcribe little to none of its own viral RNA. This research sought to give insight on the lack of knowledge when it comes to looking at how transcription works in living cells.…show more content… Which made it possible to measure the rate of H1V-1 RNA transcription and even track single RNA molecules over a period of time. This shows how MS2 is efficient because it is able to detect RNA with exceptional sensitivity and its properties allow us to see and conclude results that would not normally be available for us to examine with other technologies that are used
