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Article Review on Chronic Caffeine

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Submitted By dixieangelec
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The name of this article was “Chronic caffeine intake reverses age-induced insulin resistance in the rat: effect on skeletal muscle Glut4 transporters and AMPK activity”. The hypothesis of this study was that chronic caffeine intake reverses aging-induced insulin resistance in rats. The study included other aspects in order to rule these other aspects out of the cause of the insulin sensitivity. This meant that nothing else could be the cause of the insulin sensitivity. Wistar rats were used to test whether caffeine intake affected aging-induced insulin resistance. The experimental groups were three groups of rats treated with caffeine. These three groups were of different ages: 3 months, 12 months, and 24 months. The control groups were three groups of the same ages used in the experimental groups, but these were not treated with caffeine. The manipulations occurred in separating the rats into age groups and separating them into groups that get caffeine and those that do not get caffeine. Everything else in this study was constant including the amount of caffeine given and the amount of time it was administered.
This was an accurate test of the hypothesis. The hypothesis wanted to test caffeine intake and aging induced insulin resistance. This required groups of different ages to be tested. These groups also needed to each have their own control group to be able to determine any difference in what was being tested. The dependent variable was glucose disappearance and the independent variable was age and caffeine intake.
The results indicated that caffeine completely reversed the aging-induced insulin resistance in both 12 months and 24 months experimental groups. The caffeine did not affect insulin sensitivity in the 3 months group enough to be significant. Because the caffeine was only administered to the experimental groups, the control groups were not affected by the caffeine at all. The effect of caffeine on Glut4, AMPKα1, and AMPKα1 Thr172 phosphorylation expression in skeletal muscle was also tested. The Glut4 transporters decreased significantly; however, chronic caffeine intake restored Glut4 to normal levels. AMPKα1 levels were greatly decreased in 12 month and 24 month rats, but this was not caused by caffeine intake. The AMPKα1 Thr172 phosphorylation was not changed through aging or through the intake of caffeine.
The effect caffeine intake had ono cortisol, epinephrine, and norepinephrine levels was also tested. Aging increased cortisol levels as the levels in the 12 month and 24 month rats were higher. Both epinephrine and norepinephrine levels were decreased. While these changes occurred, none were caused by chronic caffeine intake.
Weight was also measured and it was found that chronic caffeine intake did not significantly modify body weight in any of the different age groups. However, the caffeine did reduce visceral fat mass in the 12 month group of rats. This meant that the results of caffeine intake increasing insulin sensitivity were not caused by weight loss or any other aspects. These results prove the hypothesis of this study.
Rats are physiologically similar to humans. Some characteristics both share are: warm-blooded, similar hormones controlling the body, nervous systems work in the same way, and react similarly to infection and injury. Therefore, this study could be used as a parallel for a study on how caffeine would affect insulin sensitivity in humans.
One thing the knowledge that this study produced changed, is the thought that caffeine causes weight loss. The article referred to the thought and that it was a widely accepted thought. While this study was not directly testing caffeine intake and weight loss, it was one of the variables that needed to be ruled out as a cause of insulin sensitivity. This indirectly stated that weight loss was not a direct result of caffeine intake.
While many people do not like rats, some even being terrified of them, rats can be very useful to the study of science and physiology. As it was already mentioned, rats and humans are similar in their physiological reactions and other aspects. This study has proven that a study can be done on rats and the results could possibly be used to explain physiological views about humans and different problems that humans face. Some issues rats are known to be studied for are high blood pressure and heart disease. Possibly, in the near future, rats can be helpful in discovering remedies for many other problems as well.

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