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Brine Shrimp Lab

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Question:Will adjusting the pH of a marine environment to acidic neutral or basic, affect the lifespan of brine shrimp?

Hypothesis:
If I adjust the pH of a brine shrimp environment, then the environment with the lowest pH will have the shortest lifespan.

Research: Brine shrimp are fresh water invertebrates. Adult male shrimp grow up to 0.3-0.4 inches long, while females grow up to 0.4-0.5 inches. They are capable of surviving in fresh and slightly brackish water. Scientist believe that the shrimp were originally freshwater sea life that adapted to salt.
Brine shrimp shrimp are commonly found in Great Salt Lake. They play a huge part in the migration of bird because without them as a food source, they migration wouldn't …show more content…
Many fishermen hatch their own to be able to feed the fish at the the shrimp’s prime nutritional value. Once hatched the shrimp are to kept at best a pH of 8-9, no less than 5, and no greater than 10.

Materials:
Brine shrimp take, 2 liters of distilled water, air pump, flask, 2 tablespoons of marine salt, 1tsp of brine shrimp eggs, Epsom salt, spoon, overhead lamp, yeast, hot plate, Logger Pro, pH Gauge, Temperature probe, 6 bowls, lime juice, and Sharpie

Procedure:
Hatching
Fill the tank with 2 liters of distilled water dissolve 2 tablespoons of marine salt in water on hot plate leave on hot plate till salt is dissolved allow water to cool till degrees celsius add 1 teaspoon of brine shrimp eggs to the solution place under over head lamp allow eggs to hatch in solution for 24-48 hours, scraping the sides with the spoon every 30 minutes pH Adjusting separate the hatched shrimp into 6 separate bowls label the bowl (A1, A2, B 1, B 2, N 1, N 2) in the bowls labeled A1 and A2 add 1 tsp of lime juice in the bowls labeled B1 and B2 add 4 tablespoons of Epsom salt and 1tbl spoon of baking soda observe and test the pH and temperature over a period of 5 days
Add ½ teaspoon of yeast to each brine shrimp

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