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Catalase Reaction Lab Report

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3.2.4 Investigating Enzyme Action
The effect of catalase concentration on reaction rate with Hydrogen Peroxide
3 March 2016
Nazka Nurbyek

Abstract
The purpose for this reaction was to study the effect of catalase concentration on reaction rate with Hydrogen Peroxide. Baker’s yeast in the amounts of 0.1 g, 0.2 g, 0.3 g, 0.4 g, and 0.5 g were used as a source of catalase. Yeast was suspended in 1.5% Hydrogen Peroxide solution (H2O2) and reaction rate with catalase was measured using a gas pressure sensor to monitor oxygen gas produced in kPa/sec. Data was collected using Vernier Logger Pro® software. Results showed that a clear correlation between the concentration of catalase and rate of reaction was not demonstrated. Data from experimentation …show more content…
Put on all safety equipment.
2. Start Logger Pro® software on computer.
3. Connect the LabQuest Mini to the computer using the USB cable.
4. Connect the Gas Pressure Sensor to CH 1 of the LabQuest Mini.
5. Set up the laboratory apparatus:
• Measure out 50 mL of 1.5% H2O2 into a 125 mL Erlenmeyer flask.
• Carefully place a stir bar in the flask.
• Place a magnetic stirrer on the base of a ring stand. Use a clamp to fasten the flask to the ring stand as shown. Position the flask at the center of the magnetic stirrer.
• Test the stirring speed by placing it on 80 rpm. Stop the stirrer.
• Use the plastic tubing with two Luer-lock connectors to connect the two-hole rubber stopper assembly to the Gas Pressure Sensor as shown in the image. About one-half turn of the fittings will secure the tubing tightly. The valve connected to the stopper should stay closed during this investigation.
6. Complete the steps below quickly to complete your test reaction.
• Using a micropipette, add 100 µL of enzyme suspension to the contents of the …show more content…
• Turn the stirrer to 80 rpm.
• Start data collection.
6. Stop data collection at 200 seconds.
7. Save the data.
8. Repeat steps 1-5 for 0.2 g, 0.3 g, 0.4 g, and 0.5 g.
Safety:
• Do not allow pressure to exceed 130 kPa. If it does, the rubber stopper will likely pop off.
• Wear chemistry goggles, lab coat, closed-toe shoes, and long pants.
• Tie hair back.
Results
A clear correlation between the concentration of catalase and rate of reaction was not demonstrated. There were two trials for each reaction, and the average slope was measured in kPa. The average slope of the standard reaction was m = 0.0322. The average slope with 0.1 grams of yeast was m = 0.062625. The average slope with 0.2 grams of yeast was m = 0.0023975. The average slope with 0.3 grams of yeast was m = 0.002065. The average slope with 0.4 grams of yeast was m = 0.03587. The average slope with 0.5 grams of yeast was m = 0.051025. According to these reactions, the results were inconclusive.
Standard Reaction trial 1
Please disregard m = 0.04556. It was the wrong slope due to a technical error. Standard Reaction trial

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