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Catecholase Lab Report

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The purpose of this lab was to determine the effect of temperature and pH on the reaction rate of Catecholase. The substrates of catecholase are catechol and oxygen, and it is an enzyme that speeds up chemical processes. Enzymes work best under optimal conditions, and we tested the reaction rate of catecholase with substances containing different temperatures and pH levels. pH measures the acidity or alkalinity of a substance, and is measured on a scale from 1 to 14. Temperature is the measurement of heat found in a substance. We performed two separate tests to determine how varying pH levels and temperature affected the reaction rate of catecholase. For our temperature test, three test tubes were filled with the same amount of water, and ten drops …show more content…
For the pH test, five vials were filled with clear substances with different pH levels, and we added ten drops of catecholase to each vial. Similar to the temperature tests, we then measured the intensity of color in each tube. If the substance is warmer, then there will be a darker shade of color, and if the substance is basic, than the shade will be darker.

Overall, our hypotheses were not supported. In fact, for our first graph, the temperature we predicted to have the darkest shade of color (86.7 C) had the least amount of color. The darkest shade of color was obtained in the middle temperature (26.2). In our pH data, our hypothesis was partially supported. For our first four bars, as the substances became more basic, the shade of the color got darker. Where the data strayed from our hypothesis was on our last bar (10, 3). The substance with a pH of 10 lost color compared to the substance with a pH of 8. Both graphs depict that catecholase works best under optimum conditions. For pH, the optimum condition was around 8, and once the pH reached 10, the catecholase was not able to react as fast. High pH levels can cause a stable enzyme to unfold, and lose its properties and

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