Chapter 1

Problem and its Background

Introduction

Due to poverty and lack of sufficient medical support from the government, majority of the Filipinos resort to cheaper alternative source of antibiotics. Increasing demands for commercial antibiotics result to increase in the prices of the needed medicines.

Scientist has been the forefront of searching for plants that contain active ingredients against many strains of bacteria. Researches revealed that some plant species can cure skin diseases, diarrhea, cough, and other infections. Plants then become not only source of food but it is also a source of available medicine. Plants have different uses and every single one of them is important, especially herbs. Life cannot be the same without plants.

This is for the reason that the researcher endeavors to determine the antibacterial property of avocado extract against certain strains of bacteria. The active ingredient will be obtained and used as a treatment in the study.

The researcher chose this topic because the researcher wants to learn if the growth of the Staphylococcus Epidermidis when it is placed with the avocado extract. The researcher became interested in this topic because the researcher likes to do experiments that can help the world through this research.

Theoretical Framework The Active ingredients of an avocado extract is good for the skin, it has different vitamins like vitamin A, B1, B2, B5, D, E, and more. This is good today because of the violent rays coming from the sun.

Conceptual Framework Input Process Output
|Avocado oil |
|Staphylococcus Aureus |
|Bacteria Check list |
|Preparation of materials |
|Extraction |
|Analysis of Staphylococcus |
|Epidermidis |
|Statistical treatment of the data |
|Extraction Process and Concentration |
|of Avocado Extract and its Effect on |
|the Growth of Staphylococcus Aureus |

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Statement of the Problem The study aims to compare the avocado extracts to the growth of staphylococcus Epidermidis. 1. What can be the growth of Staphylococcus in different temperature? 2. What can be the growth of Staphylococcus in a pure avocado extract? 3. What can be the growth of Staphylococcus in a dilute avocado extract?

Hypothesis Ho: there will be no difference in the growth of Staphylococcus in the concentration of an avocado extract.

Assumption of the Study 1. Avocado extract have the capability to stop the growth of the Staphylococcus Aureus. 2. The Staphylococcus Aureus has an equal and opposite reaction to the avocado extract. 3. The Staphylococcus will still grow even with the concentration of the avocado oil.

Significance of the study This research would have Significance to the following: Students. The result of this research will help the students prevent themselves from having cough, diarrhea, and other sickness that bacteria in the skin can cause. Teacher. The result of this research will help the teachers to teach students another value that they can absorb by using and treating themselves with the avocado oil. Economy. The result of this research will help the economy not only helping them with a cheap extraction of an avocado fruit and killing the skin bacteria, and they can sell this in the market and our economy will grow because of that.

Scope and Limitation The researcher focused only in the Staphylococcus Aureus to use as an experimental research. The researcher only used avocado fruit to limit the growth of the research.

Definition of Terms Avocado. This fruit is use for the dependent variable in the experimentation. StaphylococcusAureus. It is a type of staphylococcus that is a skin bacterium. Extraction. It is the process where the researcher will use this in the experimentation.