...An experimental method for determining pyrantel pamoate (PYR) via an isocratic reversed phase HPLC method with UV detection was previously optimized by Solich et al. In our experiment, we determine the PYR concentration in an old sample of a novel, anthelmintic raccoon bait. Chromatography was performed using a phenyl-hexyl column with a mobile phase of 0.5% triethylamine at pH 9.0 and acetonitrile 55 : 45 (v/v) at a flow rate of 1.0 ml min-1. For pyrantel determination, UV detection was performed at 290 nm. Analysis of the chromatographic study of the pyrantel base peaks showed that PYR makes up 2.05% of the raccoon bait by weight. Introduction Pyrantel pamoate is widely used in a variety of anthelmintic drugs, in the pharmaceutical and veterinary industries, that are designed to fight parasitic worm infections in both humans and animals. Raccoons...
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...London School of Engineering and Materials Science Laboratory report writing instructions DEN101 - Fluid Mechanics 1 Flow Rate Measurement Experiment A. Student Student Number: 1234567 Version 2.0, 27 November 2010 Template for Word 97-2003 Abstract This document explains what is expected in your Fluids 1 lab report. The sections that should be covered are outlined and a structure you could follow is proposed. Detailed advice on how to edit the report is given. The document concludes with the marking criteria for this lab report. Table of Contents Abstract 2 1. Introduction 3 1.1. Writing 3 1.2. Editing and formatting 3 1.3. Content of the introduction 4 2. Background and theory 4 3. Apparatus 4 4. Test 4 5. Experimental procedure 4 6. Results 5 7. Discussion 5 8. Conclusions 5 9. References 5 10. Appendix A: Marking criteria 6 Introduction Before starting to write a report, you should think about what is your audience. Am I writing for colleagues who want a lot of detail how it is done, or am I writing for my boss who just wants an executive summary as he has no time for details? In general, there is not a single type of audience and we have to make our writing suitable for the detailed read, as well as the fast perusal. To understand what is required from you in this report, please have a look at the marking criteria in the Appendix. 1 Writing To limit...
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...Chromatography INTRODUCTION IN THIS LAB, WE WILL BE EXPRESSING THE GREEN FLUORESCENT PROTEIN (GFP) IN BACTERIA AND PURIFYING IT USING COLUMN CHROMATOGRAPHY. THE SPECIFIC TYPE OF CHROMATOGRAPHY WE WILL BE USING IS HYDROPHOBIC INTERACTION CHROMATOGRAPHY (HIC). THE FOLLOWING IS INFORMATION FROM BIO-RAD INC., THE SUPPLIER OF THE REAGENTS: GFP has several stretches of hydrophobic amino acids, which results in the total protein being very hydrophobic. When the supernatant, rich in GFP, is passed over a HIC column in a highly salty buffer (Binding Buffer), the hydrophobic regions of the GFP stick to the HIC beads. Other proteins which are less hydrophobic (or more hydrophilic) pass right through the column. This single procedure allows the purification of GFP from a complex mixture of bacterial proteins. Loading the GFP supernatant onto the chromatography column When students load the GFP supernatant onto their columns, it is very important that they do not disturb the upper surface of the column bed when performing the chromatography procedure. The column matrix should have a relatively flat upper surface. A slightly uneven column bed will not drastically affect the procedure. However, subsequent steps of loading, washing, and eluting should minimize disrupting the column such that beads "fluff up" into the buffer. When loading the GFP supernatant onto the column, the pipette tip should be inserted into the column and should rest against the side of the column...
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...Torres 1 Luis A. Torres Group #11 USC Chemistry 322b Formal Lab Report 6th November 2015 I. II. Enzymatic Resolution of 1-Phenylethanol and Diastereomer Analysis Objective/Abstract Enzymatic transesterification reaction was performed to study the resolution of diastereomers using 1H-NMR analysis. The stereo-selectivity of acylase I, an enzyme, for a 50:50 racemic mixture of 1-phenylethanol was determined. In the first of a two-step reaction, 1-phenylethanol was reacted with vinyl acetate with the help of acylase I to form an ester, unreacted 1-phenylethanol, and vinyl alcohol. The unreacted 1phenylethanol was separated from the ester by column chromatography and confirmed by thin-layer chromatography (TLC). In the second reaction, the unreacted 1-phenylethanol was reacted with (R)-(-)-acetoxyphenylacetic acid to form a diastereomer ester. In the latter reaction, four different 1-phenylethanol samples were used in order to compare 1HNMR data of the resulting diastereomer esters and determine which enantiomer of the 50:50 racemic mixture was preferred by acylase I. Those four samples were: (1) racemic 1-phenylethanol, (2) unreacted 1-phenylethanol, (3) (R)-1-phenylethanol, and (4) (S)-1phenylethanol. After 1H-NMR analysis, it was found that the (S)-1-phenylethanol was preferred by acylase I. III. Introduction and Background Information Scheme 1: Reaction of 1-phenylethanol with vinyl acetate in the presence of the enzyme acylase I to produce 1-phenylethyl...
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...The oxidized form of the mediator hexacyanoferrate (III) accepts the electron, forming the reduced form of the mediator, hexacyanoferrate (II). The Advantage test strip employs the electrochemical principle of biamperometry. The monitor applies a voltage between two identical electrodes, which causes the reduced mediator formed during the incubation period to be reconverted to an oxidized mediator. This generates a small current that is read by the monitor. If controls do not fall within the acceptable range after one repeat 1 Do not report the patient results (this would be if the operatoris running controls for the purpose of checking the meter; otherwise, the QC lockout would not have allowed the patient to be tested prior to getting the controls in acceptable range). 2 Review the test policy and procedure. 3 To get a trade, take all of the reagents and supplies out of the drawer and take the entire GTS to the High Volume Lab of Chemistry, 5th floor McCullough Bldg. 4 If feasible for the testing site, the Nurse Manager/designee or Clinic Manager can authorize the borrowing of a GTS unit from another location. cont. NOTE: The proper comment code (09) identifying a temporary GTS unit is being used should be entered after the patient result is displayed and before it is "entered" by the operator. Point of Care should be notified that the assigned GTS unit is not performing satisfactorily. 5 Control testing should be repeated with the borrowed monitor, and, if acceptable...
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...ULTRAVIOLET/VISIBLE SPECTROSCOPY PURDUE UNIVERSITY INSTRUMENT VAN PROJECT ANALYSIS OF PLANT PIGMENTS USING PAPER CHROMATOGRAPHY AND VISIBLE AND/OR UV SPECTROSCOPY (1-31-96) INTRODUCTION We have seen that all cells must constantly consume fuel molecules to maintain themselves, grow, and reproduce. Fuel molecules such as glucose constitute an immediate source of energy for biological work that can be released by catabolic cell processes. However it is necessary that life on earth have a constant source of energy that can be harvested and used to generate complex fuel molecules from simple starting materials. The ultimate energy source upon which all life forms depend is visible light from the sun. Light energy must first be transformed into chemical(bond) energy before it can be utilized by the living cell. This transformation is achieved only in the cells of green plants and certain bacteria. In green plants it is coupled with a transformation of matter in which relatively low-energy compounds, carbon dioxide and water, are converted into high energy chemical molecules that become subunits of carbohydrates. There are four different pigment groups present in leaves of photosynthesizing plants. Studies indicate that only the chlorophyll IS involved in the actual absorption of light energy and later conversion to chemical energy of living cells. The other pigments also absorb light energy, but it is transferred to the chlorophyll for conversion to chemical energy. Biochemists have...
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...Chemist can make them fit by scientifically analyzing the evidence. Chemistry, biology, materials science, and genetics to analyze clue found at the scene of the crime, on the victims or in the bodies of the bad guys. Forensic Chemists go into a case with many unknow pieces of the crime scene they need to analyze to determine the nature of each sample. Most Forensic Chemists work in a lab. It is rare for private labs to do this kind of work so most of the time these labs are associated with Local, State, or Federal law enforcement agencies. From local Medical Examiner’s labs to state of the art FBI labs, Forensics Chemists often provide the strongest evidence in court against the defendants. They have many different types of test and methods they use to figure out what the samples mean. Each crime scene brings new types of clues and samples so a Forensic Chemist must always be thinking of ways to analyze the evidence. Some of the more common test for optical testing, X-ray spectroscopy, UV, and infrared. For separations analyses, HPLC, gas chromatography and thin-layer chromatography. In a typical day a Forensic Chemists could use as many different sciences such as chemistry, genetics, biology and Mass spectrometry. Crime shows, novels and movies have made...
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... . . . . . . . . . . . . . . 2.4.2 Diet . . . . . . . . . . . . . . . . . . . . . . . 2.4.3 Using Drugs to Reduce Detection Times . . . 3 Test Methods 3.1 Substances that are Detectable . . . . . . . . . . . 3.2 DrugAlert . . . . . . . . . . . . . . . . . . . . . . . 3.3 Gas Chromatography . . . . . . . . . . . . . . . . . 3.4 Gas Chromatography / Mass Spectrometry . . . . . 3.5 Hair testing . . . . . . . . . . . . . . . . . . . . . . 3.6 High Performance Liquid Chromatography . . . . . 3.7 ImmunoAssay . . . . . . . . . . . . . . . . . . . . . 3.7.1 Radio ImmunoAssay (aka AbuScreen) . . . 3.7.2 Enzyme Multiplied Immunoassay Technique 3.7.3 Fluorescence Polarization ImmunoAssay . . 3.8 PharmChek . . . . . . . . . . . . . . . . . . . . . . 3.9 TestCup . . . . . . . . . . . . . . . . . . . . . . . . 3 11 13 13 13 15 16 16 16 17 17 18 18 18 19 19 21 21 21 22 24 24 25 25 25 26 26 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4 CONTENTS 3.10 Thin Layer Chromatography . . . . . . . . . . . . . . . . . . . 26 4 Test...
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...CLC204 – Analytic Chemistry Experiment Quantitative Analysis of an Unknown Liquid Sample Objective: To be familiar with Ultra Violet Spectrophotometer (UV) and Gas Chromatography (GC) used in chemical analysis UV Abstract In this report, people who consumed tom yum soup suffered from nausea and vomiting due to copper poisoning which was found to leach from a pot into the soup under high heat and acidic condition. It was also suspected that an organic compound was present in the soup which enhanced the absorption of copper in consumer. Hence, as an analytical chemist, we have to use UV to determine the actual concentration of copper standards and blank using external calibration standards. The result of the test solution was measured by comparing it with the calibration of copper. Introduction The goal of this experiment is to obtain the concentration of copper in a known solution. Therefore, in this experiment, we will be using UV to measure the absorbance of the solution. A spectrophotometer is used to measure the amount of light that a sample absorbs. Ultraviolet (UV) light is an electromagnetic radiation with a wavelength in the range of 10nm to 400nm and energy from 3eV to 124Ev. With a shorter wavelength as compared to visible light, UV light is able to penetrate more readily through obstacles. Its name came from a spectrum which humans identify as the colour violet. UV light is invisible, but it can be seen indirectly when it makes other substances...
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...Plant Pigments & Absorption Lab SBI4U Metabolic Processes Lab 1 Kevin Salwach P.1 Rm. 208 De Cat November 5 2013 Lab 1 Pigments & Absorption Introduction Autotrophs, a group of organisms to which plants belong, obtain their own food and energy through ways other than hunting and ingestion. Almost all plants use photosynthesis to obtain their energy. Using the CO2 in the air that surrounds them, plants can create the energy needed to survive. The process is split into two parts; the “light” reactions occur by using light from the sun to activate electrons in the photosystem to produce adenosine triphosphate (ATP) the main requirement for metabolism. After this has occurred, the “dark” reactions occur where ATP is reduced to coenzymes in several complex cycles, and with the help of CO2, glucose is produced. This is a simplified explanation of course, as the entire photosynthetic process contains many different reactions and cycles. Overall, this is a very effective way of obtaining energy, and allows a whole manner of organisms to thrive almost anywhere on Earth. Purpose The purpose of this lab is conduct three experiments that answer the questions: - Where to plants conduct photosynthesis? - What photosynthetic pigments do they use? - What wavelength of light works best for photosynthesis? Materials -Microscope -Elodea -Microscope slide / cover slip -Spinach leaves -Mortar / pestle -Distilled water -80% acetone -Sand -Filter paper -Coleus...
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...their MW and PDI by size exclusion chromatography (SEC) using a calibration curve. The MW dependence on conversion will be investigated. The effect of the initiator concentration on polymer MW will be examined. Materials. Azobisisobutyronitrile (AIBN) and toluene (HPLC) were purchased from Sigma-Aldrich and used as received. Styrene (Sigma-Aldrich) was purified by passing through a silica column to remove inhibitors. CDCl3 was purchased from Cambridge Isotope and was used as received. Methods. Reaction procedure. To a vial charged with freshly purified styrene (10 g) was AIBN (0.7g, 0.35g or 0.17g) added at room temperature. The mixtures are then heated at 80˚C in an oil bath. Seven aliquots of the reaction mixture are removed at fixed time intervals (e.g., 0, 20, 40, 60, 80,100 min, 1 week) and analyzed by 1H NMR spectroscopy for conversion and SEC for polymer MW and PDI. Sample preparation for SEC analysis. An aliquot of the reaction mixture (3mg) is dissolved in THF(1mL) and was filtered through a syringe membrane filter prior to injection into SEC column. Sample preparation for 1H NMR analysis. An aliquot of the reaction mixture (0.1-0.5 mg) is dissolved in CDCl3 (1mL) and is transferred into a NMR tube for conversion analysis. Lab report write-up. Summarize and discuss your experimental results and describe the characterization techniques you used for this lab. The following questions should be addressed in the report. How does SEC characterization...
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...Narcotics and Criminal Justice CRJ 311 Forensics Instructor: Paul Stein December 3, 2012 Narcotics and Criminal Justice We hear the word Narcotic and most everyone knows this means a mind altering drug of some type but it is what we do not know that can hurt us. The Merriam-Webster online dictionary tells us that a Narcotic is the following: “1a: a drug (as opium or morphine) that in moderate doses dulls the senses, relieves pain, and induces profound sleep but in excessive doses causes stupor, coma, or convulsions b: a drug (as marijuana or LSD) subject to restriction similar to that of addictive narcotics whether physiologically addictive and narcotic or not 2: something that soothes, relieves, or lulls”. The government has used science to come up with a list of narcotics and classified them based on several factors and this paper is intended to cover those factors as well as how the law uses forensics to find the evidence needed to proceed within a court of law. One of the first things a court of law has to use is the knowledge of a drugs ability to cause dependency. This means that a person cannot function in a normal manner without the use of the drug and to go without the drug causing withdrawal symptoms. The government also uses information to understand the pharmacological effects of drug based on science as well as other scientific information about each drug. The government also keeps records of whether certain drugs cause a physical dependency or...
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...all other oil/gas relative equipments based on the approved Codes. Design, any required fabrications and modifications. Updating all the related drawings, drafting and as built, (P&ID drawings, Piping drawings, and Mechanical drawings). Preparing all the required reports (daily, weekly, and annually reports). Summary of Qualifications Earned value management, supported by a solid grasp of the fundamental components of Project Controls including scope definition, cost estimating, change management, cost management, scheduling, project reporting, earned value analysis and risk management • Over ten years’ experience teaching and performing research at the university level • Over 15 years’ experience in capital projects management, overseeing a variety of oil and gas fields, from extraction to processing and shipping. • Used Natural gas treating solutions, including amine plants, Triethylene glycol dehydration units and mol sieve dehydration units. P&ID development • 15 years’ experience in progressively senior roles in the oil and gas industry. • Experience with corrosion engineering, welding and painting • Experienced in Gas Chromatography...
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...http://www.labcompliance.com/tutorial/methods/default.aspx?sm=d_d TutorialHome | Contact Us | Newsletter | Usersclub | Books | Audio Seminars Seminars Audio Seminars Video Seminars Workshops Literature Books SOPs Validation Examples Free Literature Glossary Usersclub Intro Log-in Register Preview Renewal Tutorials Risk Management Practices Computer Validation Part11 Method Validation ISO 17025 Lab Equipment Qualification Good Laboratory Practices About About Labcompliance Contact Labcompliance Scope Tax/Bank Information All come with 10+ Best Practice Documents: SOPs, Checklists, Examples Transfer of Analytical Procedures According to the New USP Chapter <1224> With SOPs, templates and examples for easy implementation March 21, 2013 Quality by Design (QbD) for Analytical Method Development and Validation Learn how to design robustness for easy transfer and to avoid OOS situations Recorded, available at any time Validation of Analytical Methods for GLP and Clinical Studies Learn how to design, prepare, conduct and document for FDA compliance Recorded, available at any time Eight Steps for Cost-effective Laboratory Compliance Up-to-date overview, hot topics and trends. Recorded, available at any time Verification of Compendial Methods according to the New USP Chapter <1226> Understand the new risk based approach and and get real world case studies for testing Recorded, available...
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...information on accurate drug testing, and the types of drugs that are tested in the workplace. BBB wants information regarding if the drug tests are just as accurate with all drugs tested for. The Better Business Bureau uses the information provided to grade a business. They determine if the drug testing companies meet Better Business Bureau accreditation standards. The Better Business Bureau is a nonprofit organization that was founded in 1912. It exists to gather and review information on businesses based on the information, and consumer reports that the BBB has received, and give the business a grade. The Better Business Bureau grades businesses with letter grades from A+ (highest) to F (lowest). The BBB will usually explain the major reasons that raised or lowered a business’ grade. The consumers then utilize this information to decide if they want to do business with a certain business. Consumers are also the ones who generally file reports, and complaints to the Better Business Bureau about a business. The goal for the Better Business Bureau is to review drug testing companies and to give them a grade for consumers, such as business’ that are on the market for a drug screening company. The Better Business Bureau wants information on which drugs are tested for, and how accurate those drug tests are. The BBB is...
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