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Daphnia Case Study Answers

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Set out materials on a sizeable, open surface.
Choose and create three to five concentrations of the Original, Diet, and Caffeine Free Coke. These will be used to make the solutions for which will have Daphnia placed into them to allow heart rate measurement.
(How to create concentrations: For example (in mL), a twenty five percent solution would be represented in the ratio 1:4. The twenty five percent would consist of the liquid that is desired to be used; in this case, it is soda. The remaining seventy five percent would be water. In a basic 10 mL solution, 2.5 mL would entail the soda, and 7.5 mL would be comprised of water.)
For each of the concentrations that have been chosen, measure the heart rate of four to seven individual Daphnia. …show more content…
Use one of the plastic transfer pipettes to pick up an individual Daphnia, and transfer it to the container filled with liquid. Put the pipette just underneath the surface of the solution in the Petri dish and pipette carefully to avoid introducing air bubbles below the shell of the Daphnia.
Place the chosen container under the compound microscope and focus on the Daphnia (starting at the lowest power objective and moving on to the higher objectives as needed) so that you can see the beating heart. If the Daphnia is moving around excessively, remove a bit of the solution surrounding it. Gently move the Daphnia if it is in a bad position for examination using the pipette to turn it, or by picking it up and replacing

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