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Daphnia Magna Research Paper

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Introduction The Daphnia magna, more commonly known as the water flea, is a tiny freshwater crustacean. Its clear exoskeleton and jointed appendages point to its classification as an arthropod. This is further evidenced by the location of its heart—the dorsal region. The Daphnia magna’s importance in the aquatic food chain can be attributed to its essential place in the diet of young and adult fish (Elbert, 2005). The Daphnia magna belongs to Class Branchiopoda, which are free-floating crustaceans that. It is also, more specifically, a member of Genus Daphnia (Ebert, 2005). Branchiopods always have flattened, partially leaf-like, legs. As a branchiopod, the Daphnia magna belongs to the Order Cladocera, which is protected by a calcified …show more content…
A depression slide was obtained, and a small amount of petroleum jelly was smeared into the well. A living Daphnia magna was drawn up through the modified pipet and placed into the well of the depression slide. A KimWipe was used to absorb the excess culture fluid from the well, leaving the Daphnia magna hindered by the petroleum jelly and preventing excessive motion. Two drops of culture fluid were then used to cover the Daphnia magna to prevent dessication. The slide was then placed on the stage of a dissection microscope and left for one minute to give the Daphnia magna time to become calm. The heart of the Daphnia magna was then identified and observed. The number of heart beats in ten seconds was counted, and that number was multiplied by six to get the beats per minute for the room temperature reading at 23°C. A KimWipe was the used in order to remove the culture fluid from the …show more content…
Effect of Acetylcholine of the Heart rate of Daphnia magna
Time (minutes) Heart rate (beats/minute)
Time Zero- Baseline 252 (beats/minute)
1 minutes 180 (beats/minute)
2 minutes 168 (beats/minute)
3 minutes 150 (beats/minute)
4 minutes 144 (beats/minute)
5 minutes 138 (beats/minute)

Experiment 3: Effect of Adrenaline/Epinephrine on the Heart rate of Daphnia magna
As can be seen in Table 3, the heart rate of the Daphnia magna increased during the first two minutes of exposure to epinephrine, but then sharply declined during the third minute. Following its decrease, another sharp increase in heart rate was seen during the fourth minute, the remained constant until the end of the experiment.
Table 3. Effect of Adrenaline/Epinephrine on the Heart rate of Daphnia magna
Time (minutes) Heart rate (beats/minute)
Time Zero- Baseline 180(beats/minute)
1 minutes 228 (beats/minute)
2 minutes 258 (beats/minute)
3 minutes 240 (beats/minute)
4 minutes 282 (beats/minute)
5 minutes 282 (beats/minute)
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