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Enzyme Jelly Lab

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In the experiment “Enzyme Jelly Lab”, it was discovered that the hypothesis was rejected. The hypothesis being: If temperature affects enzyme function then fresh pineapple is the best to use when making jello. The rationale behind this hypothesis was that the bonds within the collagen of the fresh pineapple could withstand the temperature up to 70 degrees Celsius. This would allow bonds to repair themselves after cooling. When combined with hot water, the helices form a 3-D structure and is unraveled which would then form gelatin. In canned pineapple, the temperature of 100 degrees Celsius is too high for the peptide bonds to hold together and this would permanently break the bonds. This would not allow the solution to cool and set. After revealing the results, it was discovered that the canned pineapple was the successor out of the two types of pineapple to create jello. This occurred because the canned pineapple …show more content…
(McCoy, 2014) Some of the physical differences between using fresh pineapple versus canned pineapple could include how the pineapple has been shaped. Canned pineapple can be bought in rings, crushed, diced; whereas, fresh pineapple can be made into any given shape. Canned pineapple may be more enriched in juices for preserving reasons than fresh pineapple would provide.
Other fruit that have the same proteolytic properties as pineapple are papaya and kiwi. The action of the fruits is the same. They all contain proteases which is most likely to repel pests. These enzymes are used by digesting any pests that may attempt to feed on the fruit (Ansell, 2009). These other fruits would not be able to form gelatin along with pineapple because of the high concentration of proteases present, it would break down collagen not allowing gelatin to

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