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Experimentation Critique

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The title of this experiment is Probiotic capsules do not lower plasma lipids in young women and men. The objective of this investigation was to clarify the effect of probiotic bacteria administered in capsule form on plasma lipid concentrations.

Independent variableThe independent variable is the probiotic capsules. Probiotic bacteria have been defined as live microorganisms, which when consumed in adequate amounts, confer a health effect on the host. These bacteria have been hypothesized to enhance the immune response, treat antibiotic or travelers diarrhea, improve gastrointestinal health and reduce fecal enzymes implicated in cancer initiation. Probiotic bacteria are incorporated into dairy foods such as milk and yogurt or packaged as capsules for human consumption.

In vitro experiments have suggested several mechanisms for the cholesterol-lowering effect of probiotic bacteria such as L. acidophilus and Bifidobacterium Bacteria may directly remove cholesterol by incorporating it into the bacterial cell membrane, binding cholesterol to the cell surface or assimilating cholesterol during growth. In addition, bacteria may produce bile salt hydrolase and deconjugate bile acids. The resulting bile acids co-precipitate with cholesterol and are excreted in the feces, thereby reducing cholesterol through fecal excretion and through the synthesis of additional bile acids from cholesterol.

The majority of studies examining the effect of probiotic bacteria on plasma lipids have employed fermented milk products as the vehicle of administration. Although most studies have reported a hypocholesterolemic effect , not all investigations support this result. The logistics of procuring and storing a fermented milk product for daily consumption and the dietary modification required to accommodate the additional calories contained in fermented milk products have prompted an exploration of alternative methods for ingesting probiotic bacteria.

Capsules containing freeze-dried probiotic bacteria are a more convenient and calorie-free medium with a refrigerated shelf life of up to 12 months. Probiotic capsules and...

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