...Discussion In order to figure out what family an organism belongs to all the way down to the species, a scientist will perform many tests. Each test done will help to eliminate other bacteria. Once a scientist knows the species, the right steps can be taken to help out people if they get contaminated with the bacteria. When I received my unknown bacteria, I started of doing a Gram Stain. This test revealed to me that I had a Gram negative bacilli in a random arrangement. This step is very important to figure out what type of family the bacteria belongs to. Gram negative bacteria have a thin peptidoglycan layer and a waxy outer layer. This is important to know when it comes down to giving antibiotics. Most bacteria are susceptible to antibiotics...
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...Introduction In 1946, Tide was launched by a US company named Procter & Gamble (P & G). In 1949, Tide has successfully established itself as an international brand. They have a range of products which includes household detergent such as Tide Liquid, Tide Powder and many more (Procter & Gambler 2012). Besides, Tide was not just another laundry product; it is recognized as the washing miracle. It was the first heavy-duty synthetic detergent which revolutionized from the traditional laundry soaps as it does not leave the clothes stiff and dry. Tide has successfully changed the way families did their laundry. This innovation of using synthetic compounds has greatly reduced the time and effort consumers spent while doing their laundry (American Chemical Society 2007). Tide captured more than 30 percent of the laundry market in the early 1950s and therefore emerged to be the top selling detergent (Back to EurekAlert! 2006). 1 Situation Analysis According to Lamb et al. (2012), situational analysis includes SWOT analysis that affects the performance of an organization. Organizational resources such as financial resources, production costs, marketing skills, technology, employee capabilities and brand image should be taken into consideration. Environmental scanning is also essential as it affects the organization’s future as it can help in identifying market opportunities and threats. Additionally, PESTLE analysis will be conducted to analyze the relationship between...
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...cheek smear looked like the cells were stained, rather than the background as it was explained in the experiment. It shows in the up close cheek smear, as the yeast smear didn't seem to retain the red color. It was however easier to make out the shapes of the cells. The plaque smear, retained some of the red dye but not in any sort of pattern in which to clearly be able to see cells. B. The bacterial morphologies, also known as the shape of the cells, were very easy to see with the crystal violet stain. The slides showed cocci and bacillus shaped cells. On the wet mount cheek smears it was hard to make out any shapes, but in the direct staining slides you could make out cocci shapes on the cheek and yeast smears. You could also make out some cocci on the indirectly stained yeast slide. C. Direct staining uses dyes that stain the cells, such as the crystal violet, methylene blue, or basic fuchsin. They are considered a basic dye that are used for the direct stains that only colors the cytoplasm of the cell and leaves the background clear. This happens because the cell membrane is negatively charged and the dye is positively charged. The...
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...MEETING CONSUMER’S DEMAND: CUT ABOVE THE REST OMO’s strength is its high quality stain removing power. This market leader would fit the needs of our consumers who seek to find the best possible solution to make the clothes ‘white and shining’. As NE consumers press on stain removal, bleach properties that cleans the clothes without much hard work, our OMOSOAP with its perceived- high quality would be aspirational and for once affordable to the consumers. We will manufacture the soap bar as a cuboid in OMO’s symbolic colors- Red and Blue. As mentioned in section one, this will make our product ‘standout’ when consumers are washing with OMOSOAP in a group, and hence strengthen the effect of OMO’s brand identity. PACKAGING: We package the soap using our brand name OMO so that the core benefits of our product (stain removal) can be conveyed explicitly. In addition, for packaging we use the same colors (red and blue) and logo as used for OMO detergent. This we believe would encourage emotional response and better customer recall (Kotler, 1997, p. 38). The advantages of our product are: ·Core product: The soap removes the toughest of stains and makes the clothes stain-free and shining. ·Actual product: According to the evaluations by the NE consumers towards detergents, they attach great importance to the quantity of foam produced in the detergent, by which they judge the ability...
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...Removing Stains and Grease An Official Requirement in Research II Rico Emmanuel A. Pineda Arnieca Lulu Kim R. Mamayson Jhunaiza C. Santos Researchers Mr. Lucky Sonny A. Ligsanan Research Adviser I. Introduction Washing dishes is one of the major house chores our parents are facing every day. One of hardest part of washing dishes are the tough stains and grease that is all over the common kitchen tools we use in our everyday life. Dishwashing liquid is a great help to minimize the time of removing those tough stains and grease on our kitchen tools and it totally removes all the dirt of common kitchen tools. Imagine yourself washing the dishes without using any dishwashing liquid, how will you easily remove those stains and grease? This study shows how a simple organic material that can be easily found elsewhere can be turned into something as useful as dishwashing liquid. Background of the Study This research aims to produce a dishwashing liquid out of Gumamela petals that will remove the stains and grease in common kitchen tools. This study will make use of Gumamela petals which contains mucilage that effectively removes stains or grease present in common kitchen tools. Significance of the Study The study will produce a new kind of dishwashing liquid that is completely organic. It will make use of Gumamela petals mixed with water and calamansi extract to produce a dishwashing liquid that is chemical free yet effective in removing stain or grease...
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...Materials…………………………………………………………………………………………11 Procedure…………………………………………………………………………………….......12 Flowchart…...……………………………………………………………………………………13 Statistical Treatment……………………………………………………………………………..13 CHAPTER IV: DATA ANALYSIS AND INTERPRETATION……………………………14 Results and Analysis of Data…………………………………………………………………….14 CHAPTER V: SUMMARY, CONCLUSIONS, AND RECOMMENDATIONS…………..18 Summary ………………………………………………………………………………………...18 Conclusions…...………………………………………………………………………………….19 Recommendations………………………………………………………………………………..20 LIST OF TABLES Table 4.1 – Experiment Run Table 4.1.1 – Results Table 4.2 – Criterion for Collection of Data APPROVAL SHEET This research study entitled “Using Grapefruit Extracts as an Alternative Cloth Stain Remover” prepared by Jason Alexander Lapina, Carlos Ivan Obnial and Ren Jeroham Vargas and in partial fulfillment for the requirements in Research Academic AY 2014-2015 and is...
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...bundle of bills for the largest bill, and how much equaled $1,000,000.00 of the largest bill printed. So below, I will outline my research and support my result on a graph for further explanation. 1.) The largest currency circulated to the public is the $100.00 bill. (www.treasury.gov) 2.) A bundle equals 100 of $100.00 bills. (www.answers.com) 3.) It takes 10 bundles of $100.00 bills to make $1,000,000.00. 4.) The weight of a single bill is 1 gram. (www.enchantedlearning.com) 5.) The weight of a bundle of 100-$100.00 bills is 100g. 6.) The weight of 10 bundles of $100.00 bills is 10,000g. 7.) Converting 10,000g to lbs.= 22lb (www.convertunits.com) 8.) The weight of $1,000,000.00 is 22lbs in $100.00 bills. If you follow the graph below it shows the weight of $1,000,000.00 if you were to carry it with the highest currency printed of $100.00 which is a total of 22lbs or 10,000 grams. If you would change your currency to the single dollar it would be a weight of 2207.00 lbs. or 1001073 grams, my assumption would change to no, as I would not be able to lift that much weight. References: www.treasury.gov www.answers.com www.enchantedlearning.com...
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...Introduction Lever is a very simple device that can measure the mass of an object. In this experiment, we are going to make a lever that consists of ruler, 50 gram weight and string that could measure the mass of a battery. We will get the mass by using the formula of moment (moment = distance×force) and based on the data that we obtain from the experiment. Moment is a force that can make object turn around if there is a pivot, for example the see-saw. Objective * To understand the use of moment formula (moment = distance×force) * To use the principle of moment to calculate an unknown weight. Materials * Meter stick × 1 * Battery × 1 * 50 gram weight × 1 * String × 1 * Scissor × 1 * Tape × 1 * Retort stand and clamp × 1 Procedure 1. Cut the string into half with scissor 2. Fold one of the strings into half and then tie a knot at the end of the string. Repeat this for another string. 3. Tape the battery on the string and then tape the 50 gram weight on another string. 4. Clamp the meter stick on the retort stand with clamp on the middle (50cm) of the stick. 5. Hang the battery on one side of the meter stick and the 5g weight on another side. 6. Move the 5g weight to make the meter stick balanced. 7. Loosen the clamp to test if the stick is balanced 8. Find the distance between battery and pivot, and also the distance between 50g weight and pivot. 9. Use Moment = distance × force and Distance1...
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...30 Day Cleansing and Fat Burning System The night before you get started: Weigh yourself and record your weight Take your measurements and record them. Take before pictures; front and side. Everyday Routine on your program no matter if it’s a Shake Day or Cleanse Day Breakfast Lunch Bedtime 1 ounce of Ionix Supreme Take ½ hour apart 1 natural accelerator 1 natural accelerator 2 Isaflush with 8 ounces of water *Can be increased if needed *Drink a minimum of a gallon of water (128 ounces daily) Extremely important in the beginning of this program Sample Calendar Sunday Monday Tuesday Day 5 Shake Day Day 12 Shake Day Day 19 Shake Day Day 26 Shake Day Day 6 Cleanse Day Day 13 Cleanse Day Day 20 Shake Day Day 27 Shake Day Day 7 Cleanse Day Day 14 Cleanse Day Day 21 Shake Day Day 28 Shake Day Wednesday Day 1 Shake Day Day 8 Shake Day Day 15 Shake Day Day 22 Shake Day Day 29 Shake Day Thursday Day 2 Shake Day Day 9 Shake Day Day 16 Shake Day Day 23 Shake Day Day 30 Shake Day Friday Day 3 Shake Day Day 10 Shake Day Day 17 Shake Day Day 24 Shake Day Begin Maintenance Program Saturday Day 4 Shake Day Day 11 Shake Day Day 18 Shake Day Day 25 Shake Day Or continue 30 Day program THIS IS JUST A SAMPLE. YOU MUST CONTACT YOUR COACH TO PLAN YOUR PROGRAM Shake Days Replace 2 of your meals with Isalean Shakes and enjoy one healthy meal between 400-600 calories. Use 2 scoops of Isalean shake or one packet with 8 oz. water and ice in a blender. You can also use a hand-shaker...
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...GOLD BUYING PROCEDURE Undocumented/treasure AU Bars PRIOR OR BEFORE IMPLEMENTATION A TABLE MEETING IS A MUST FOR M.O.A SIGNING BY BUYER & SELLER. For sponsored project (Seller is not the one paying the Contingency Fee/ Intermediary/ies paid the fee ) both Parties have agreed that a day before implementation, First Party (SELLER ) will hand over the _____ grams of sliced gold grams from subject gold bars to Second Party ( BUYER ) as “commitment” and to be given to the buyer’s verifier at the venue a day prior ( before ) to implementation as “COMMITMENT” rest in three MAJOR reasons: Assurance gold slice quantity varies as follows : METRO MANILA 100 grams LUZON 200 grams VISAYAS & MINDANAO 300 grams. 1. Avoid last minute back out. ( pag-urong ng seller sa araw ng bilihan / paglabag sa pinirmahang kasunduan ) 2. Avoid switching ( pagpalit ng item na peke ) 3. Serve as key for the Buyer as assurance for expenses incurred ( in caase of seller’s failure ) ( ang sliced grams of gold ay magsilbing pantapal sa nagastos ng BUYER, kung sakali man magkaroon ng hindi pagkatuluyan sa transakayon sa anumang dahilan nilabag ng SELLER sa araw ng buying/implementasyon. ) MIMINUM BUYING PER IMPLEMENTATION 6.2 KG. ( 5 BARS ) 12.5 KG ( 3 BARS ) 74.6 KG. ( 1 BAR ) Transaction/s wherein the Seller or intermeiaries pays for the Contingency Fee for its implementation...
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... 1 60 12) 0.35 x 2.5 = ________ 7. 0.7 13) How many tablets will be needed to give the following dose? The tablets are labeled 1.2 mg. The order is for 0.6 mg. ________ tablet(s) 14) Reduce the following as far as possible: 124 76 = ___________________________ 15) Express the following division to the nearest tenth: 5.7 1.46 = __________________________ 16) Solve the following equation. Express answer to the nearest tenth. 30 x 50 x 10 x 415 1 550 1250 1 = ____________________ Write the following metric measures using their abbreviations and notation rules. 17) Four hundredth of a gram ___________ 18) One and three tenths milligrams ___________ 19) Three hundred thousand units ___________ 20) Three and a half micrograms ___________ 21) Twenty seven hundred units ___________ Convert the following metric measures: 22) 700 mg = ________ g 23) 0.45 mg = ________ mcg 24) 1 L = ________ mL 25) 10cc = ________ mL 26) 1300mcg = ________ mg...
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...technique was gram staining. This technique was used to determine if bacteria is present. Then, it had to be determined if the organism was gram positive or gram negative. During the staining procedure there is a primary stain of crystal violet, the mordant gram iodine, a decolorizer acetone alcohol, and a counterstain of safranin. After the steps are completed the gram positive organism remains purple and the gram negative bacteria shows as a reddish-pinkish organism. Gram staining came about by Hans Christian Gram. He was trying to see cocci in the lungs of individuals who lost their lives to pneumonia. Once Gram gathered a collection of materials to complete a stain, he figured out a way to view the bacteria in the lungs. The stain of crystal violet retained to the people who were infected with pneumonia. He realized that his stain worked with envisioning various types of bacteria pertaining to “cocci of supportive arthritis following scarlet fever”. Because Gram finally discovered how to distinguish between a positive bacteria organism from a negative bacteria organism, many doctors are able to figure out ones illness at an early stage, once a doctor does so they might be able to use...
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...Discussion a) Gram Staining Escherichia coli is a Gram-negative rod- shaped bacterium. Initially the bacterium is stained purple by the primary stain, crystal violet. The alcohol which is used as a decolourising agent washes away the outer membrane and allows the crystal violet complex to wash out from the thin peptidoglycan, making the cell colourless. When the counterstain, safranin is applied, they appear pink. Staphylococcus aureus is a Gram-positive bacterium and appear in cocci grape-like clusters. They have a very thick peptidoglycan layer that can retain the purple stain from crystal violet. The decolourising solution has no effect on this bacterium besides a slight drying effect on the wall, The pink counterstain does bind the cells but it is not seen because covered by a darker purple colour primary stain. Bacillus subtilis are Gram-positive rod shaped bacteria that appear as small chains or single cells. They have thick peptidoglycan layer that can retain the purple stain from crystal violet. Saccharomyces cerevisiae are circular which occur singly or in a group. They do not respond to gram staining due to their cell wall that consists of glucan and mannoproteins. They usually bind to the original dye, look blue but they are not Gram-positive. Thus, they are Gram non-reactive. Water isolates found in rod shaped and they are Gram-negative bacteria that stains pink after safranin applied due to the thin peptidoglycan layer. b) Acid fast staining Mycobacterium...
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...to isolate our particular bacteria. Daily Notes Day 1 – Chose unknown bacteria #70, which is a broth culture. It is cloudy and yellow in color, with sediment gathered at the bottom. It has a strong odor, and I question whether I have smelled it before. The first test I’m doing is a Gram stain. Doing 3 slides – all air dried and heat fixed. Also streaking 2 plates today: TSA plate, and 2nd plate will be based on the results of the Gram stain. Either: MSA= isolates S. aureus, inhibits G-, EMB= fecal coliforms, G- growth (selective isolation G- rods), MAC= promotes G- growth. I am worried that I won’t be able to achieve isolation of separate colonies from the broth culture when I streak the plates. Viewed results of Gram stain with microscope under 100x oil immersion. Results of 1st Gram stain were inconclusive, so staining again with another slide. Many more cells on this slide. 2nd Gram stain = G- bacilli (rods), confirmed with Claudia. Decide to streak 2nd plate = MAC since I believe I have a G- bacteria. Day 2 – Achieved isolation on both TSA and MAC agar’s. Definite growth on MAC means I do have a G- bacteria. Will now perform a 2nd Gram stain from TSA plate to confirm Gram status; confirmed that it is G- . After looking at the chart, decided to try to narrow down options by doing an oxidase test. There are 6 oxidase positive bacteria, and 8 oxidase negative bacteria. Oxidase test results = negative. Inoculated a TSA slant today before leaving. Day 3...
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... As I walked into the house, I could almost taste the eggnog that would be waiting for me on the table. Eyeing the plate of cookies, I forced myself to follow my Gram up the stairs to the attic. The cookies could wait. I had come to help my Gram set up her miniature Christmas village. For Christmas each year I would give my Gram a new building, ice skater, or tree to add to her village. Her face would light up as she found the perfect place for the village’s newest addition. About two weeks before Christmas I would arrive at my Grams house to help her set up the village. I would watch in amazement as she placed each individual piece in the same exact place as she had the year before - slightly altering the village to welcome the newest addition. This was my favorite Christmas tradition when I was a child, and I looked forward to it every year. Although I live a distance away from my Gram, I visit frequently and wish I had more time to do so. She has always been completely engaged in my daily life, and it would seem strange to not see her on the first day of school each year, camera in hand, eager to take my picture. My Gram acts as a second mother to me, and I can always count on her for support. I have grown to trust her completely and she always seems to be the person who is there to help me. My Gram is the first person I go to for help and I can talk to her about virtually anything. Regardless of the subject of our conversations, she loves the fact that I talk to...
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