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Health Assess

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Submitted By kearasimmons
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Ke’Ara Simmons
Lab, Week # 6

SPECIFIC IMMUNITY

Introduction The purpose of this lab experiment is to conduct a series of biochemical test to identify the bacterium responsible for the symptoms the 4 year old is suffering from. Also to see if her body has specific immunity to any distinct species or strains of invader.

Case Study: A 4-year old girl was suffering from severe, watery diarrhea, lethargy, and nosebleeds. Emergency room personnel reported a fever of 101 F, blood pressure of 110/70 and heart rate of 72. Blood and stool samples were taken. Urine samples contained blood. The blood work revealed an acidic pH and abnormally low levels of platelets and erythrocytes. High levels of creatinine and urea were observed. This organism was cultured from various body fluids.

After conducting a series of biochemical test the bacterium responsible for the young girl suffering was Escherichia coli

Procedure

To repeat the experimental procedure one will need Chemicals such as Gram Stain, Acid from Glucose, Gas from Glucose, Lactose Fermentation, Indole Production, Lysine Decarboxylase, and Malonate Utilization. Tools such as: Buner, loop tool, incubator, microscope, and Access to the virtual unknown lab software.

Observations and Results

| Result + or - | Appearance after Incubation | Gram Stain | Negative | N/A | Acid from Glucose | Positive | Yellow | Gas from Glucose | Positive | Yellow; bubbles present | Lactose Fermentation | Positive | Yellow | Indole Production | Positive | Cherry-red ring floating above | Lysine Decarboxylase | Positive | 24hr. yellow; 48hr. purple | Malonate Utilization | Negative | Green; rarely any growth |

Bacterium identified
Assigned Unknown: Escherichia coli
Identified Unknown: Escherichia coli

Discussion
Identify the bacterium – Assigned Unknown: Escherichia coli; Identified Unknown: Escherichia coli
Describe the expected immunological course of events during the infection, development of disease, and recovery of this patient, including the following information: - Humoral Immune response
The means by which specific clones are formed and activated – The cells are formed in bonne marrow, but go to other parts of the body where they are activated. B cells are activated in the bone marrow while T cells are activated in the Thymus. The body generates billions of antibodies by being discrete on the DNA 1. the role of T-cells and B-cells in the process – T- cells attack viruses; Bells from the antibodies, B-cells attack bacteria. 2. the classes of immunoglobulins active at each step in the immunoactivation process – IgG, IgM, IgA, IgE, and IgD when the B cells are active. 3. the way immunological memory is generated. – because the host already has antibodies produced after the first exposur. It’s from memory B cells
Conclusion

After all these labs I think I am getting better, when I went to find out my unknown there was only one to choose from. I must be conducting my test better to have my unknown come down to the main bacterium. All in all I enjoyed doing this lab even thought I made mistakes. I am getting better at virtual unknown lab software.

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