Premium Essay

History Of Paper Chromatography

Submitted By
Words 827
Pages 4
Chromatography which literally means “color writing” was invented by Mikhail Tsvet, a Russian botanist in 1903 while studying the coloring materials in plant life. It is defined as a laboratory technique utilized by scientists for the separation of organic and inorganic mixtures into their respective components in order to analyze, identify, purify and/or quantify the mixture or components. There are different types of chromatography which are use including liquid chromatography, gas chromatography, ion-exchange chromatography, paper chromatography and thin-layer chromatography. In consonance with these, while each type of chromatography uses different methods to separate compounds, all forms work on the same principle. They all have a stationary …show more content…
In paper chromatography, the filter paper (stationary phase) holds the components until all the solvent (mobile phase) dissolves them and through the aid of capillary action, it travels up the filter paper. Moreover, the solvent can only move the components if they are soluble in it. Also, the component will travel up the filter paper at a rate that is determined by its affinity to the filter paper and solvent. Since each component has its own solubility with the solvent and its own affinity to the solvent and filter paper, they can be separated in multiple ways by using mixtures of different solvents and different filter papers. Therefore, the separation of components in paper chromatography highly depends on their solubility with the solvent and their affinity to the solvent and filter …show more content…
First is the application/treatment of the sample, then this is followed by the development of the chromatogram done by allowing the mobile phase to move up the paper. Lastly, the retention factor value is calculated using Equation 1.1. The retention factor (Rf) is the ratio of the distance travelled by the component or the solute to the distance travelled by the solvent. If the Rf value of a solution is equivalent to zero, then the solute remains in the stationary phase and it is motionless as a result. Whereas if the Rf value is equal to 1, then the solute has zero affinity for the stationary phase and it moves with the solvent front. Therefore, the retention factor’s value is also a representation of the movement or migration of solution in relation to the

Similar Documents

Premium Essay

Voltametry

...------------------------------------------------- Gas chromatography Gas chromatography (GC), is a common type of chromatography used in analytical chemistry for separating and analyzing compounds that can be vaporized without decomposition. Typical uses of GC include testing the purity of a particular substance, or separating the different components of a mixture (the relative amounts of such components can also be determined). In some situations, GC may help in identifying a compound. In preparative chromatography, GC can be used to prepare pure compounds from a mixture.[1][2] In gas chromatography, the mobile phase (or "moving phase") is a carrier gas, usually an inert gas such as helium or an unreactive gas such as nitrogen. The stationary phase is a microscopic layer of liquid or polymer on an inert solidsupport, inside a piece of glass or metal tubing called a column (a homage to the fractionating column used in distillation). The instrument used to perform gas chromatography is called a gas chromatograph (or "aerograph", "gas separator"). The gaseous compounds being analyzed interact with the walls of the column, which is coated with a stationary phase. This causes each compound to elute at a different time, known as the retention time of the compound. The comparison of retention times is what gives GC its analytical usefulness. Gas chromatography is in principle similar to column chromatography (as well as other forms of chromatography, such as HPLC, TLC), but has several notable...

Words: 12827 - Pages: 52

Premium Essay

Glycemic Control Monitoring

...Patrick Sullivan HSCI 331 Journal Critique The Potential for Glycemic Control Monitoring and Screening for diabetes at Dental Visits Using Oral Blood: By: Shiela M. Strauss, Mary T. Rosedale, Michael A. Pesce, David M. Rindskopf, Navjot Kaur, Caroline M. Juterbock, Mark S. Wolff, Dolores Malaspina, and Ann Danoff Vol. 105, No. 4, pp. 796-801. doi: 10.2105/AJPH.2014.302357 Diabetes has become a major problem in the United States. More and more Americans are affected each day because simple healthy lifestyle routines are not met. There are many undiagnosed Americans who do not know that they have or are susceptible to diabetes due to family history of the disease or their very own dietary disorientation. Glycemic control, a way to monitor blood sugar through diet and blood source, has proven to help people with diabetes and those who are pre-diabetic. The major public health issue which this research concerns is how to get early diagnosis and treatment through a more primary intervention for those who already have or are susceptible to diabetes. The study is important because Type 2 diabetes and other forms of the disease are preventable. Patients can only be helped if they are diagnosed, understand susceptibility, and are aware of the health problem. There is a known fact that most patients visit their dental provider more often than a primary care provider (PCP), some reasons being that dental visits are inexpensive and less invasive. The objective...

Words: 646 - Pages: 3

Free Essay

Identification of Statins in Rice Fermented with Monascus Species from the Museum of Natural History

...FERMENTED WITH MONASCUS SPECIES FROM THE MUSEUM OF NATURAL HISTORY Jude Carlo J. Muca1, Reigna S. Romero1, Ricardo R. Santos², Roberto Z. Yuseco3, Florence M. Blanco1, Lei Anne C. Carolino1, Engkhuan Chew1, Ericka Joy B. De Guzman1, Jordan Carlo S. Galang1, Jin-Gu Lee1, Lawrence Y. Maliwat1, Marixie Ann Q. Manarang1, Jeshua Caleb B. Miole1, Michael Henry B. Piano1, Lou Anthony S. Sico1, Mark Lester I. Tolenada1, Mellanie B. Victoria1 1 Medical Student, Angeles University Foundation, Angeles City 2009; ²Head, Department of Biochemistry, School of Medicine, Angeles University Foundation, Angeles City 2009; 3 Faculty, Department of Biochemistry, School of Medicine, Angeles University Foundation, Angeles City 2009; ABSTRACT Pharmaceutical intervention through statin drugs is the most common way of slowing down the adverse effects of heart disease due to cholesterol deposition by regulating the activity of HMG-CoA reductase which catalyzes conversion of HMG-CoA to mevalonate. In order to seek alternatives for commercially available statin drugs, this study idnetified the statins that can be produced by fermentation of rice by Monascus strains available at the Museum of Natural History (MNH). It also shall serve as a set-off point for further studies regarding the use of fermentation products for treatment of certain ailments, such as heart disease. A total of eight (8) isolates available at the Museum of Natural History (MNH) of the University of the Philippines – Los Baños...

Words: 3052 - Pages: 13

Premium Essay

Abdutheddude

...including scope definition, cost estimating, change management, cost management, scheduling, project reporting, earned value analysis and risk management • Over ten years’ experience teaching and performing research at the university level • Over 15 years’ experience in capital projects management, overseeing a variety of oil and gas fields, from extraction to processing and shipping. • Used Natural gas treating solutions, including amine plants, Triethylene glycol dehydration units and mol sieve dehydration units. P&ID development • 15 years’ experience in progressively senior roles in the oil and gas industry. • Experience with corrosion engineering, welding and painting • Experienced in Gas Chromatography...

Words: 1290 - Pages: 6

Free Essay

Forensic Science

...Felicia Lee; Walter Dinivan; Madam Jetkor Miang Singh; Roberto Calvi; Buck Ruxton & the Jigsaw Murders; Acid Bath Haigh; 2005 London Bombings; "Brides in the Bath"; Gareth Williams; The Woodchipper Murder WEEK 2- CHEMICAL ANALYSIS IN FORENSIC SCIENCE Quote "Actus non facit reum nisi mens sit rea" The act is not culpable unless the mind is also guilty. Learning Objective(s) At the end of this topic, you should be able to: 2A. Atomic Structure & Spectroscopy 1. Explain the structure of the atom and Bohr's model; 2. Differentiate between emission and absorption spectroscopy; 3. Explain the chemistry behind EDX and SEM-EDX; 4. Explain the chemistry in NAA; 2B. Molecular Spectroscopy & Chromatography 1. Apply the principles of chromatography; 2. Describe the methods used in the analysis of compounds; 3. Explain the chemistry behind the methods for the analysis of compounds; 4. Identify which method is more effective for the analysis of compounds. Synopsis An introduction to spectroscopy and the structure of atoms. The comparisons...

Words: 1646 - Pages: 7

Premium Essay

Forensic Science History Paper

...Forensic Science History Paper CJS/215 December 7, 2015 Jim Anderson Forensic Science History Paper A segment of the Forensic Science in the Criminal equity organization that the vast majority acquainted with these days, however, few individuals know how this particular science came to be. Overall, legal science is intended to utilize experimental standards to clarify obscure parts of a wrongdoing. Through the utilization of uncommon tests and lab gear that permits researchers to recognize decisively different parts of a bit of confirmation in an illegal behavior. On the off chance that you occupied with finding out about how this procedure came to fruition, you have to peruse the recorded data underneath. Here is a brief history of criminological science to demonstrate to you how this practice came to fruition throughout an inordinate length of time. The most punctual legal researchers were doctors who were called upon to give a supposition as to the reason for death in people. Presently the majority of the legal researchers are agents that get pieces of information from the wrongdoing scene and investigate. It utilizes exceedingly created innovation to reveal investigative confirmation in an assortment of fields. Modern forensic science has a broad range of applications. It is utilized as a part of common cases, for example, falsifications, misrepresentation or carelessness. The most widely recognized utilization of criminological science is to research criminal cases...

Words: 1013 - Pages: 5

Premium Essay

Narcotics and Criminal Justice

...but in excessive doses causes stupor, coma, or convulsions b: a drug (as marijuana or LSD) subject to restriction similar to that of addictive narcotics whether physiologically addictive and narcotic or not 2: something that soothes, relieves, or lulls”. The government has used science to come up with a list of narcotics and classified them based on several factors and this paper is intended to cover those factors as well as how the law uses forensics to find the evidence needed to proceed within a court of law. One of the first things a court of law has to use is the knowledge of a drugs ability to cause dependency. This means that a person cannot function in a normal manner without the use of the drug and to go without the drug causing withdrawal symptoms. The government also uses information to understand the pharmacological effects of drug based on science as well as other scientific information about each drug. The government also keeps records of whether certain drugs cause a physical dependency or mental dependency or both as well as information concerning each drug with its history, patterns of abuse as well as what the risk is to the health of the public. Information also concerning other drugs or substances used that may enhance a drugs performance (Justia US Law, Indiana Code 2010). Narcotic is the term used by both many states and the federal government for drugs that are both...

Words: 1556 - Pages: 7

Free Essay

Explosives Forensics

...Explosive Forensics Daniel Glass Criminal Investigations Professor Barbara Bailey August 21, 2011 Abstract Explosive investigations have changed over the years, from first being searched out by visual with law enforcement to today’s advanced technology devices. It has gone from knowing the type of explosive to being able to identify a specific manufacturer. Even today it continues to advance and evolve to become more helpful in finding and punishing the individuals responsible. History Bomb detection has been as issue throughout history since the inception of mass detonation devices. At first the main method was through looking at the shape of the device since the explosives were large and had a defined look based on the materials, detonation device and such. Usually it was done after the fact by searching for the components, explosive residue and such to look for a specific trait of a bomb maker or type of explosive used. It has always been the driven by the research for our military to create more weapons and ways to keep them from detection. It started with humans looking at the objects, and then advanced to using X-rays and such, to detection dogs, dolphins, and so on up to today’s vapor detection systems. Current Reasons for Advances in Detection The following instances are just a basis for the push to expand our capabilities to detect explosives. It is a needed capability to protect our citizens. * 1983 Marine Barracks Bombing Lebanon * 1988 Pan...

Words: 1173 - Pages: 5

Free Essay

Analytical Chem

...Chemistry David Harvey DePauw University Boston Burr Ridge, IL Dubuque, IA Madison, WI New York San Francisco St. Louis Bangkok Bogotá Caracas Lisbon London Madrid Mexico City Milan New Delhi Seoul Singapore Sydney Taipei Toronto McGraw-Hill Higher Education A Division of The McGraw-Hill Companies MODERN ANALYTICAL CHEMISTRY Copyright © 2000 by The McGraw-Hill Companies, Inc. All rights reserved. Printed in the United States of America. Except as permitted under the United States Copyright Act of 1976, no part of this publication may be reproduced or distributed in any form or by any means, or stored in a data base or retrieval system, without the prior written permission of the publisher. This book is printed on acid-free paper. 1 2 3 4 5 6 7 8 9 0 KGP/KGP 0 9 8 7 6 5 4 3 2 1 0 ISBN 0–07–237547–7 Vice president and editorial director: Kevin T. Kane Publisher: James M. Smith Sponsoring editor: Kent A. Peterson Editorial assistant: Jennifer L. Bensink Developmental editor: Shirley R. Oberbroeckling Senior marketing manager: Martin J. Lange Senior project manager: Jayne Klein Production supervisor: Laura Fuller Coordinator of freelance design: Michelle D. Whitaker Senior photo research coordinator: Lori Hancock Senior supplement coordinator: Audrey A. Reiter Compositor: Shepherd, Inc. Typeface: 10/12 Minion Printer: Quebecor Printing Book Group/Kingsport Freelance cover/interior designer: Elise Lansdon Cover image: © George Diebold/The Stock Market Photo research:...

Words: 88362 - Pages: 354

Free Essay

Defeating Drug Screens

... . . . . . . . . . . . . . . 2.4.2 Diet . . . . . . . . . . . . . . . . . . . . . . . 2.4.3 Using Drugs to Reduce Detection Times . . . 3 Test Methods 3.1 Substances that are Detectable . . . . . . . . . . . 3.2 DrugAlert . . . . . . . . . . . . . . . . . . . . . . . 3.3 Gas Chromatography . . . . . . . . . . . . . . . . . 3.4 Gas Chromatography / Mass Spectrometry . . . . . 3.5 Hair testing . . . . . . . . . . . . . . . . . . . . . . 3.6 High Performance Liquid Chromatography . . . . . 3.7 ImmunoAssay . . . . . . . . . . . . . . . . . . . . . 3.7.1 Radio ImmunoAssay (aka AbuScreen) . . . 3.7.2 Enzyme Multiplied Immunoassay Technique 3.7.3 Fluorescence Polarization ImmunoAssay . . 3.8 PharmChek . . . . . . . . . . . . . . . . . . . . . . 3.9 TestCup . . . . . . . . . . . . . . . . . . . . . . . . 3 11 13 13 13 15 16 16 16 17 17 18 18 18 19 19 21 21 21 22 24 24 25 25 25 26 26 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4 CONTENTS 3.10 Thin Layer Chromatography . . . . . . . . . . . . . . . . . . . 26 4 Test...

Words: 16323 - Pages: 66

Free Essay

Charcoal

...SEALANT COSTS Sir, I was interested to read the practice paper by Dr Bonetti on Evidence not practised: The underutilisation of preventive fissure sealants.1 In the recently published clinical guideline by the Scottish Intercollegiate Guidelines Network (SIGN),2 it has been suggested that resin-based PFSs should be applied to the permanent molars of all children as early after eruption as possible. In other words, applications should usually take place between the ages of 6-7 years for first permanent molars and 11-12 years for second permanent molars. In 2012/13, approximately 30% of primary 7 children (mean age 11.5 years) in Scotland received PFSs.3 In Scotland, the current cost of application of PFS to unfilled permanent molar teeth within two years of their eruption is £8.15 per tooth.4 To increase the uptake of PFSs in first permanent molars from 30% to 60% in Scottish children aged 6-7 years, SIGN estimated that the incremental cost would be over £1 million. Similarly, over £1 million would be required to double the uptake of PFSs in second permanent molars in Scottish children aged 11-12 years.2 It was not possible to segregate visits incorporating treatment with visits representing routine examinations in the above estimated costs; therefore, the total cost of service provision in Scotland may have been underestimated. However, potential savings from restorations avoided are also excluded.2 Implementation of the SIGN guideline is the responsibility of each NHS board...

Words: 1182 - Pages: 5

Free Essay

Cassava Leaves as an Emulsion Surfactant for Detergents

...CASSAVA LEAVES AS AN POTENTIAL EMULSION SURFACTANT FOR DETERGENTS ____________________ A Research Project Presented to the Faculty of the Science Technology and Engineering Batasan Hills National High School IBP Road, Batasan Hills, Quezon City ____________________ In Partial Fulfilment of the Requirements for the Research III ____________________ by Borromeo, Kristine Joy R. March 2016 CHAPTER I THE PROBLEM AND ITS BACKGROUND Background of the Study Almost every human uses detergents everyday, the problem is we had gone too far in using detergents. Detergents are for cleansing that are daily in use. They are becoming more and more expensive and on demand because of their increasing purposes. In this modern age, detergents are not as effective as what like they said in ads and commercials because sometimes too much chemicals re used which can affect its effectivity. The research is done to find out an alternative solution for the problem said in detergents using cassava leaves. Cassava leaves is common inside the community. The solution aims to produce the as an potential emulsion surfactant for detergents using the cassava leaves. The product does the same job as others but is more natural because an organic specimen is added. It contains less harmful chemicals. The said product will perform important functions in detergents cleaning such as loosening emulsifying and holding suspension until it can be rinsed (Healthy Cleaning 101, 2010 ) . ...

Words: 3323 - Pages: 14

Premium Essay

Forensics Essay

...investigation. Immediately conjuring images of crime labs, ultra violet lights and high-tech computers, popular crimes shows like CSI have brought increased attention to the field of forensic science. They've also generated newfound interest in forensics careers. The term "forensic" comes from the Latin and means, simply, having to do with the law. Hence, any discipline that has any ties to the legal system is, in fact, forensic. This is why so many job titles within criminology, such as forensic psychologist, are preceded by the term. With regard to forensic science, the term is now commonly understood to refer to the application of scientific principles to questions of law. In short, it means using science to solve crime. The History of Forensic Science [edit] When In Rome The earliest roots of forensic science as we know it can be found in ancient Greek and Roman society. These western civilizations brought great advances in the medical field as well as pharmacology. Extensive knowledge was developed regarding the production, use, and symptoms of various poisons, making it possible to identify their use in previously undetected murders. Et Tu Brute? The First Autopsy In the year 44 B.C., the Roman physician Antistius examined the body of the recently slain Julius Caesar and determined that, though the dictator was stabbed 23 times, only one wound through his chest caused his actual death. This is widely regarded as the first recorded autopsy. The First Innocence...

Words: 1731 - Pages: 7

Premium Essay

Forensic Science Research Paper

...Welcome To Homicide Anthony Clay CRJ 311 Forensics Professor: Gary Naylor January 8, 2017 Forensic science, has been around for quite some time now. In this paper I will include insight into the role of forensic science, the applications of forensic science for use within the evaluation and/or processing of major crime scenes, and its role in the criminal justice system. Brief history of forensic science. Forensic science dates back all the to the 18th century. “One of the earliest pioneers of forensic science was a Spanish doctor, Mathieu Joseph Bonaventure Orfila (1787–1853)” (Gaensslen & Larsen, 2013, pg. 1.2). Mathieu is known as the father of forensic toxicology. His primary focus was in forensic science, his work influenced the development of modern day criminalistics labs. He was also was...

Words: 1514 - Pages: 7

Premium Essay

Jobs in Science

...Biomedical scientist: Job description More in this section Job description Salary and conditions Entry requirements Training Career development Employers and vacancy … Related jobs Print all pages in this section Case studies Biomedical scientist: Ann Thomas Biomedical scientist: Kathryn Owen Biomedical scientist: Roslyn Cooke Biomedical scientists work in healthcare and carry out a range of laboratory tests and techniques on tissue samples and fluids to help clinicians diagnose diseases. They also evaluate the effectiveness of treatments. Their work is extremely important for many hospital departments and the functions they carry out are wide ranging. For example, they may work on medical conditions, such as cancer, diabetes, AIDS, malaria, food poisoning or anaemia, or carry out tests for emergency blood transfusions or to see if someone has had a heart attack. Biomedical scientists can work in three areas: infection sciences; blood sciences; and cellular sciences. Infection sciences include: •medical microbiology - identification of micro-organisms causing disease and their antibiotic treatment; •virology - identification of viruses, associated diseases and monitoring the effectiveness of vaccines. Blood sciences include: •clinical chemistry - analysis of body fluids and toxicology studies; •transfusion science - determination of donor/recipient blood compatibility, ensuring blood banks are sufficient; •haematology - form...

Words: 9745 - Pages: 39