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Invertase/ Phosphatase Results

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Submitted By lulubutters22
Words 293
Pages 2
Lindsey M. Maurice-Walker
Week 6/7 Results
Biochemistry Lab
March 14, 2013

Results:
Table 1 and Table 2, given below, display the average absorbance of the samples, the amount of protein in each sample, the activity value, the specific activity, and the molar absorptivity calculated from the above equations for Phosphatase and Invertase. Table 1. Value Summary for Phosphatase | | Abs400 | mg of protein | Units (activity)(mg/min) | Specific Activity(ug/min/mg) | CYE | 0.296333333 | 0.003753 | 3.7079E-07 | 0.000099 | F1 | 0.832 | 0.000732 | 1.23686E-06 | 0.001689704 | F2 | 0.365333333 | 0.002678 | 4.8235E-07 | 0.000180116 | F3 | 0.152666667 | 0.0003375 | 1.38507E-07 | 0.000410 | Negative | 0.067 | | | | Positive | 1.304 | | | | Molar Abs. (M-1 cm-1) | 20616.67 | | | |
The values located in the column labeled, “Abs400,” were obtained by averaging the four absorbance readings of the certified yeast extract (CYE), the controls, and samples F1-F3 at 400nm. The other values were found using the equations listed in the Methods section of this report.

Table 2. Value Summary for Invertase | | Abs540 | mg of protein | Units (activity)(mmol/min) | Specific Activity (umol/min/mg) | CYE | 1.33225 | 0.003753 | 3.36E-06 | 0.000894706 | F1 | 2.782 | 0.000732 | 7.29E-06 | 0.009953591 | F2 | 2.84875 | 0.002678 | 7.47E-06 | 0.002788235 | F3 | 2.874 | 0.0003375 | 7.54E-06 | 0.022326842 | Negative | 0.093 | | | | Positive | 3.0455 | | | | Molar Abs. (M-1 cm-1) | 24604.17 | | | |
The values located in the column labeled, “Abs540,” were obtained by averaging the four absorbance readings of the certified yeast extract (CYE), the controls, and samples F1-F3 at 540nm. The other values were found using the equations listed in the Methods section of this report.

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