Title : Investigation of Action of Saliva and 3 M Hydrochloric Acid in Two Carbohydrate Solutions
Objective : To investigate the action of saliva and 3 hydrochloric acid in two carbohydrate solution
Solution A Benedict’s test:
Blue coloration turned to brick red precipitate. Reducing sugar is present in the solution A.
The coloration remained unchanged. Starch is absent in solution A.
Solution B Benedict’s test:
The blue coloration remained unchanged. Reducing sugar is absent in solution B.
The coloration turn into dark blue coloration. Starch is present in solution B.
Temperature(°C) Benedict's Test-Colour Observation
After min 5th min
(from tubes 1 - 4 into 1' - 4') After 35th min
(from tubes 1 - 4 into 1' - 4')
1 10 ml solution B
1 ml saliva 37 Blue coloration turned into translucent green. Blue coloration turned to green and finally moderate amount of brick red precipitate suspended in solution. The solution was opaque.
2 10 ml solution B
3 M HCI 37 The blue coloured solution remains unchanged. The blue coloured solution remains unchanged.
3 10 ml solution B
3 M HCI 95 White coloured translucent suspension formed in moderate amount. A very big amount of white suspension formed which is very opaque. Turned back into translucent and remaining solution was blue which is translucent.
4 10 ml solution B
1 ml saliva 95 The blue coloured solution remains unchanged. The blue coloured solution remains unchanged as well.
In the experiment, the enzyme amylase was involved. Amylase breaking down the starch suspension into maltose and maltose into glucose when HCL was added into solution B .This is because solution B was hydrolyzed and the H+ ions present break...