...Practical 3 Investigation of Action of Saliva and Hydrochloric Acid in Two Carbohydrate Solutions ____________________________________________________________________________________ Objective: Students are expected to state the objective of this experiment. Apparatus & Equipments: Boiling tubes Metal test tube racks Beaker Graduated plastic dropper Water bath, ~ 37 oC Water bath, ~ 95oC Materials: Carbohydrate solution A Carbohydrate solution B Benedict’s solution 3 M Hydrochloric acid 3 M Sodium hydroxide Procedures: 1. Prepare two boiling tubes containing 1 ml solution A and 1 ml solution B respectively. Add 1 ml Benedict’s solution to each tube. Heat both tubes together in the (~95oC) water bath for two minutes. Record the results in table 1. 2. Add a few drops of fresh solution A and B separately spaced on a white tile. On each solution, add 1-2 drops of iodine solution. Mix with pen cover. Record your observations in the table 1. 3. Pipette 2 ml solution B into each of four boiling tubes. Label the tubes 1, 2, 3 and 4 respectively near mouth of tube. Label your group name. 4. Place tubes 1 and 2 in a water bath of ~37oC. (It doesn’t matter how long you put it in at this stage as no saliva or HCl have been added yet). 5. Salivate into a small beaker till it reaches about 5 ml. 6. Step (6) and (7) is to be done approximately at the same time. Measure out 4 ml of the saliva prepared in step (4) and pipette 2 ml each into tubes...
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...Practical 3 Investigation of Action of Saliva and Hydrochloric Acid in Two Carbohydrate Solution | Objective: 1. To show the action of saliva in two carbohydrate solutions. 2. To show the action of hydrochloric acid in two carbohydrate solutions. Apparatus & Equipment’s: Boiling tubes Metal test tube racks Beaker Graduated plastic dropper Water bath,~37°C Water bath,~95°C Stop watch Test tube holder Materials: Carbohydrate solution A Carbohydrate solution B Benedict’s solution 3M Hydrochloric acid 3M Sodium hydroxide Procedures: 1. Prepared two boiling tubes with containing 1 ml solution A and 1 ml solution B respectively. 1 ml Benedict’s solution was added to each tube and heated both tubes together in the (~95°C) water bath for two minutes. Then, recorded the results in table 1. 2. Added a few drops of fresh solution A and B separately spaced on a white tile. On each solution, added 1-2 drops of iodine solution and mixed with pen cover. Recorded your observations in the table 1. 3. Pipetted 2 ml solution B into each of four boiling tubes. The tubes were labelled 1, 2, 3 and 4 respectively near mouth of tube. Labelled your group name. 4. Placed tubes 1 and 2 in a water bath of ~37°C. 5. Salivated into a small beaker until it reached about 5 ml. 6. At the same time, step (6) and (7) was to be done approximately. Measured out 4 ml of the saliva prepared in step (4) and pipetted 2 ml each into tubes 1 and 4. The contents of the tubes shook well to ensure...
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...enzymes in human cells. Above this temperature the enzyme structure begins to break down (denature) since at higher temperatures intra- and intermolecular bonds are broken as the enzyme molecules gain even more kinetic energy.Also Enzymes have an active site. This is part of the molecule that has just the right shape and functional groups to bind to one of the reacting molecules. The reacting molecule that binds to the enzyme is called the substrate.At high temperature,the active site is said to be denatured.Therefore,the active site cannot bind with any subtrate anymore.Amylase breaking down the starch suspension into maltose and maltose into glucose when HCL was added into solution B .This is because solution B was hydrolyzed and the H+ ions present break down the bond in between molecules of the solution B The HCl will denature (unfold) the amylase (as noted at the bottom of the link you provided), disrupting...
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...1. Advance Room Booking will be held as per the dates below. Same Room Booking – Female and Male Date | 23 - 26 June 2014 (Monday to Thursday) | Time | 8.30 am to 5.30 pm | Venue | Accommodation Office – (ACO) - Einstein Ground Floor | Residents are required to come to ACO to secure their rooms for the following semester during the Advance Room Booking period. Invoice collection will be from 30 June 2014 onwards at ACO. If you fail to do so during this time period your room will not be available for the following semester. If your roommate is graduating and or leaving the Halls of Residence you will need to find a new roommate. Please update ACO as soon as possible with the new roommate’s name. If you are unable to find a new roommate you will be required to fully check out from the room and ACO will reassign you to another room. When a room is totally empty the university is able to conduct preventive maintenance. There is storage space available. Boxes will be provided by ACO and you will need to return them 3 days after you check in. 2. Changing Room Policy Residents who wish to change rooms will only be considered on a ‘Case by Case Basis’ and ‘Subject to Room Availability’. Residents need to book back their same room during the Advance Room Booking period and to drop by ACO to complete an Appeal Form and submit it with a supporting letter from 1 - 2 July 2014. Only fully completed Appeal Forms with valid reasons will be considered. No appeal for change of...
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...Practical 1 Practical 1 Cell Biology Biological Studies I molecules I Practical 2 Practical 2 Cell Biology Biological Studies II molecules II Practical 3 Cell Biology Studies III Practical 4 Cell Biology Studies IV Practical 8 Cell Biology Studies VIII Practical 5 Cell Biology Studies V Practical 6 Cell Biology Studies VI Practical 7 Cell Biology Studies VII Practical 9 Cell Biology Studies IX Practical 10 Cell Biology Studies X - Experiment Description Page Writing of Lab Reports Identification of Biomolecules 5 13 Identification of Unknown Carbohydrate Solutions and Investigation of Action of Saliva and HCl in Carbohydrate Solution at Two Different Temperatures Investigation of the Effects of Catalase Concentration on Hydrogen Peroxide Decomposition 20 Synthesis of Starch Using an Enzyme Extracted from Potato Tuber Investigation of the Effects of Different Catalytic Conditions on Hydrogen Peroxide Decomposition Microscopy 27 Practical 6 Cell studies II Practical 7 Cell studies III Extraction of Cell Organelles by Cell Fractionation Determination of Solute Potential of Potato Cell Sap 47 Practical 8 Cell studies IV Effects of Different Treatments on Stained Potato Cells 64 Practical 9 Energetics I Respiration of Germinating Beans 67 Microscopic Examination of Cells at Various Stages of Plant Mitosis and Meiosis DNA, Mitosis and Meiosis Modelling 71 Respiration of...
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...Centre for Foundation Studies, UTAR 1 2 Chapter Scopes • Endothermic & Exothermic reactions • Enthalpy changes: ∆H of formation, combustion, hydration, neutralization, atomization. CHAPTER 5 Chemical Energetic / Thermochemistry • Lattice energy, electron affinity • Heat of fusion and vaporization • Hess’ Law • Born-Haber cycles • Calorimetry © 2006 Brooks/Cole - Thomson © 2006 Brooks/Cole - Thomson 3 Energy & Chemistry 4 Thermochemistry • Thermochemistry is the study of heat (energy) change/transfer in a chemical reaction. • ENERGY is the capacity to do work or transfer heat. • HEAT is the transfer of thermal energy between two objects because of their difference in temperature. Heat energy is associated with molecular motions. Other forms of energy light electrical kinetic and potential Heat transfers until thermal equilibrium is established. ∆T measures energy transferred. © 2006 Brooks/Cole - Thomson © 2006 Brooks/Cole - Thomson System and Surroundings 5 System and Surroundings 6 Vacuum jacket • SYSTEM – The object under study • SURROUNDINGS – Everything outside the system © 2006 Brooks/Cole - Thomson FHSC1114 Physical Chemistry open Exchange: mass & energy closed energy isolated nothing © 2006 Brooks/Cole - Thomson 1 Centre for Foundation Studies, UTAR Directionality of Heat Transfer 7 Directionality of Heat Transfer • Heat always transfer...
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...enzymes in human cells. Above this temperature the enzyme structure begins to break down (denature) since at higher temperatures intra- and intermolecular bonds are broken as the enzyme molecules gain even more kinetic energy.Also Enzymes have an active site. This is part of the molecule that has just the right shape and functional groups to bind to one of the reacting molecules. The reacting molecule that binds to the enzyme is called the substrate.At high temperature,the active site is said to be denatured.Therefore,the active site cannot bind with any subtrate anymore.Amylase breaking down the starch suspension into maltose and maltose into glucose when HCL was added into solution B .This is because solution B was hydrolyzed and the H+ ions present break down the bond in between molecules of the solution B The HCl will denature (unfold) the amylase (as noted at the bottom of the link you provided), disrupting...
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...Title: To investigate the trajectory of a small ball as it rolls off a surface which is inclined to the horizontal. Objectives: To investigate the trajectory of a two dimensional motion. Apparatus and materials: Ramp, wooden block, pendulum bob, plumb line, steel ball, wooden board, carbon paper, meter ruler, plasticine. Setup: 1. A ramp was set up at the edge of a bench. 2. A plum-line is suspended from the edge of the bench. 3. A wooden board is mounted horizontally using two clamps so that the board is situated about the bottom of the ramp. 4. A sheet of blank paper is placed on top of the board. 5. A piece of carbon paper is placed on the top of the blank paper. The ink-side of the carbon paper is facing down. 6. When a ball was released from the top of the ramp, the ball travelled through a trajectory. Theory: Let: g= 9.80ms-2 u= speed of the ball as it leaves the ramp k= constant y= vertical distance (between the bottom of the ramp and the top of the board) x= horizontal distance (between the plum-line and mark on the paper) The equation which relates x and y is yx=g1+k2x2u2+k Procedure : 1. The ball was positioned at the top of the ramp. The ball was released so that it rolls down the...
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...of the two solutions with Iodine test and ... Discussion: ... Why You Add Hydrochloric Acid in Hydrolysis of Starch. Ingestion of saliva during carbohydrate feeding by ... - SciELO www.scielo.br/scielo.php?pid=S0074-02762006000100016&script... by RR Cavalcante - 2006 - Cited by 5 - Related articles Saliva ingestion by phlebotomine during the carbohydrate ingestion phase is ... The presence of saliva in each type of solution or substrate offered, as well as ... 0.2 ml of apyrase assay buffer pH 8 (50 mM Tris/HCl buffer containing 1 mM CaCl2, ... Saliva ingestion occurred under each condition investigated, as indicated by ... Experiment: investigation of action of saliva and hydrochloric ... https://answers.yahoo.com/question/index?qid... Jun 25, 2012 - 1) Name of enzyme involved 2)specific action(s) of enzymes involved. Two hydrolytic enzymes and an epistemological–historical ... www.scienceinschool.org/2007/issue4/enzymes Science in School Sep 3, 2007 - 5 M sodium hydroxide (NaOH); 5 M hydrochloric acid (HCl); Saliva. ... To demonstrate the test methods, test all four carbohydrate solutions ... Discussion ... more concentrated than is necessary for the activity and that using a ... The authors have recommended the Fehling’s test for this investigation. What is the objectives of investigation of action of saliva and ... www.answers.com › Wiki Answers › Categories › Science › Biology ... of investigation of action of saliva and diluted hydrochloric acid in two...
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...Practical 3 Investigation of Action of Saliva and Hydrochloric Acid in Two Carbohydrate Solution | Objective: 1. To show the action of saliva in two carbohydrate solutions. 2. To show the action of hydrochloric acid in two carbohydrate solutions. Apparatus & Equipment’s: Boiling tubes Metal test tube racks Beaker Graduated plastic dropper Water bath,~37°C Water bath,~95°C Stop watch Test tube holder Materials: Carbohydrate solution A Carbohydrate solution B Benedict’s solution 3M Hydrochloric acid 3M Sodium hydroxide Procedures: 1. Prepared two boiling tubes with containing 1 ml solution A and 1 ml solution B respectively. 1 ml Benedict’s solution was added to each tube and heated both tubes together in the (~95°C) water bath for two minutes. Then, recorded the results in table 1. 2. Added a few drops of fresh solution A and B separately spaced on a white tile. On each solution, added 1-2 drops of iodine solution and mixed with pen cover. Recorded your observations in the table 1. 3. Pipetted 2 ml solution B into each of four boiling tubes. The tubes were labelled 1, 2, 3 and 4 respectively near mouth of tube. Labelled your group name. 4. Placed tubes 1 and 2 in a water bath of ~37°C. 5. Salivated into a small beaker until it reached about 5 ml. 6. At the same time, step (6) and (7) was to be done approximately. Measured out 4 ml of the saliva prepared in step (4) and pipetted 2 ml each into tubes 1 and 4. The contents of the tubes shook well to ensure...
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...TITLE OF LAB REPORT | Practical 3: Investigation of Action of Saliva and 3M Hydrochloric Acid in Two Carbohydrate Solutions | LECTURER’S NAME | Ms. Bong Siew Mee | Title: Investigation of Action of Saliva and 3M Hydrochloric Acid in Two Carbohydrate Solutions Objective: To investigate the action of saliva and 3M hydrochloric acid in two carbohydrate solutions. Results: Table 1: Observations made when two carbohydrate solutions provided in laboratory were tested with Benedict and Iodine solution. | Observations | Conclusions | Solution A | Benedict’s test: An initial blue translucent mixture turned to brick-red opaque solution and moderate amount of precipitate settled after heated at a high temperature for two minute. | Presence of reducing sugar | | Iodine test: The translucent colouration of the mixture retained its yellowish-brown colour. | Absence of starch | Solution B | Benedict’s test: The translucent colouration of the mixture remained its blue colour. | Absence of reducing sugar | | Iodine test: The initial yellowish-brown translucent mixture turned to bluish-black opaque solution when solution was mixed. | Presence of starch | Table 2: Colour reactions of Benedict’s test for saliva and 3M hydrochloric acid in two carbohydrate solutions provided in laboratory. Tube |...
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...Topic: Investigation of Action of Saliva and 3 M Hydrochloric Acid In Two Carbohydrate Solution Objective: To investigate the action of saliva and 3 M Hydrochloric Acide in two Carbohydrate Solution Apparatus & Equipment: Boiling tubes Beaker Graduated plastic dropper Water bath, (~ 37oC) Water bath, (~ 95oC) Materials: Carbohydrate solution A Carbohydrate solution B Benedict’s solution 3 M Hydrochloric acid 3 M Sodium hydroxide Iodine solution Procedures: Part 1 1. Two boiling tubes were prepared containing 1 ml solution A and 1 ml solution B respectively. 1 ml Benedict’s solution was added to each tube. Both tubes were heated together in the (~95oC) water bath for two minutes. The results were recorded in table 1. 2. Few drops of solution A and B were added separately on a white tile. On each solution, 1-2 drops of iodine solution were added. The observations were recorded in the table 1. Part 2 3. The boiling tubes were labelled 1, 2, 3 and 4. 2 ml of solution B were pipette into each of four boiling tubes. 4. Tubes 1 and 2were placed in a water bath of ~37oC to heat up the solution. 5. The saliva was salivated into a small beaker until it reaches approximate 5 ml. 6. This step was done approximately at the same time. 2 ml of saliva prepared were pipette into tubes 1 and 4. The contents of the tubes were shaken well to ensure thorough mixing. 2 ml of HCl were pipette into tubes 2 and 3. 7. Tubes 1, 2, 3 & 4 were incubated at their...
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...Title Identification of Unknown Carbohydrate Solution and Investigation of Action of Saliva and Hydrochloric Acid in Carbohydrate Solution at Two Different Temperatures Objective To investigate the action of saliva and 3 hydrochloric acid in two carbohydrate solution Apparatus Boiling tubes, Metal test tube racks, Water bath, 37-40 Degree Celsius, Water bath, 90-95 Degree Celsius, Beaker, Dropper, Wooden Holder Materials Carbohydrate solution A , Carbohydrate solution B , Benedict’s solution , 3 M Hydrochloric acid, 3 M Sodium Hydroxide Procedures 1. Two boiling tubes are filled with 1 ml solution A and 1 ml solution B respectively. 1 ml of Benedict’s solution is added into each test tube. Both tubes are heated together in the hotter (90-95°C) water bath in two minutes. The results are recorded in Table 1. 2. A few drops of fresh solution A and B are spaced separately on a white tile. I2/KI solution (iodine) is added 1-2 drops on each solution. The solution is mixed with a glass rod on the tile. The observations are recorded in the Table 1. 3. 2ml solution B is added into each of four test tubes and the tubes 1, 2, 3, and 4 is labelled respectively with labelling paper near mouth of tube. 4. Tubes 1 and 2 is placed in a water bath of 37°C. 5. A measuring cylinder is salivated till it reached 5ml. 6. 2ml of saliva is added into tubes 1 and 4. The contents of the tubes are shaked well to ensure thorough mixing. 7. 4ml of HCL is measured...
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...P16 Group Member : Lim Phei Ching Lee Jer Win Chong Hui Li Title : Investigation of Action of Saliva and 3 M Hydrochloric Acid in Two Carbohydrate Solutions . Objective : To Investigate of Effect of Salive and Hydrochloric Acid in Carbohydrate Solutions . Apparatus & Equipment : Boiling tubes , Beaker , Graduated plastic dropper , Water bath, ~ 37 oC , Water bath, ~ 95oC Materials : Carbohydrate solution A , Carbohydrate solution B , Benedict‟s solution , 3 M Hydrochloric acid , 3 M Sodium hydroxide , Iodine solution Procedures : Part 1 1. Two boiling tubes containing 1 ml solution A and 1 ml of solution B were prepared respectively . 1ml of Benedict solution was added into each tube . Both tubes were heated together in the 95°C water bath for two minutes . Results were recorded in table 1 . 2. A few drops of solution A and B were added separately on a white tile . 1-2 drops of iodine solution were added on each solution . Part 2 3. Boiling tubes were labeled 1,2,3,4 . 2 ml of solution B was pipette into each of four boiling tubes . 4. Tubes 1 and 2 were placed in a water bath of 37°C to heat up the solution . 5. Small beaker was salivated till it reached about 5 ml . 6. This step was done approximately at the same time . 2ml of saliva prepared was pipetted into tubes 1 and 4 . The contents of tubes were shaken well to ensure through mixing . 2ml of HCL was pipetted into tubes...
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...P16 Group Member : Lim Phei Ching Lee Jer Win Chong Hui Li Title : Investigation of Action of Saliva and 3 M Hydrochloric Acid in Two Carbohydrate Solutions . Objective : To Investigate of Effect of Salive and Hydrochloric Acid in Carbohydrate Solutions . Apparatus & Equipment : Boiling tubes , Beaker , Graduated plastic dropper , Water bath, ~ 37 oC , Water bath, ~ 95oC Materials : Carbohydrate solution A , Carbohydrate solution B , Benedict‟s solution , 3 M Hydrochloric acid , 3 M Sodium hydroxide , Iodine solution Procedures : Part 1 1. Two boiling tubes containing 1 ml solution A and 1 ml of solution B were prepared respectively . 1ml of Benedict solution was added into each tube . Both tubes were heated together in the 95°C water bath for two minutes . Results were recorded in table 1 . 2. A few drops of solution A and B were added separately on a white tile . 1-2 drops of iodine solution were added on each solution . Part 2 3. Boiling tubes were labeled 1,2,3,4 . 2 ml of solution B was pipette into each of four boiling tubes . 4. Tubes 1 and 2 were placed in a water bath of 37°C to heat up the solution . 5. Small beaker was salivated till it reached about 5 ml . 6. This step was done approximately at the same time . 2ml of saliva prepared was pipetted into tubes 1 and 4 . The contents of tubes were shaken well to ensure through mixing . 2ml of HCL was pipetted into...
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