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Lancefield Group Testing Lab Report

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Lancefield group testing works by serologically grouping beta-haemolytic streptococci based on their C-carbohydrates that are located within the cell wall. The specific antigen that differentiates strains of streptococci is known as the ‘M’ antigen, (Acharya, 2015). Different strains of streptococci have different m-antigens and this is the basis of Lancefield testing. Reagents 1 and 2 used in the test, are extracting agents. They detach the specific antigen from the cell allowing it to be further tested. Reagent 3 is a neutraliser which is added before mixing the sample with individual blue latex groups. 6 latex groups were used in this experiment, A, B, C, D, F and G, corresponding to the group of streptococci they identify. Next a small

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