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Live Cell Exposure System

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Submitted By oktumi
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nanoT - VaryCell TM
Micro stage for time lapse imaging and temperature mediated cellular studies.
Cells derived from mammals have an optimum in vitro growth rate between 36°C to 37°C as their in vivo physiological temperature ranges between 36.5–37.5 °C (97.7–99.5 °F) in a normal individual. Homeostasis process maintains the constancy of the organism’s internal environment in response to changes in external conditions. Thermoregulation is part of the aforementioned process as controlled by the organism’s circadian rhythm.

Figure 1: Diurnal temperature variation chart
However there are cell types which have optimum growth at different to that of human cell types i.e. insect, avian, amphibians and cold water fish cells. Ectotherms or cold blooded animal cells can grow within a temperature range between 15°C and 26°C. Insect cells has an optimal growth at 27°C and can grow between 27°C and 30°C. Avian cells has an optimal growth at 38.5°C, however they can be cultured at temperature ranging between 37°C to 38°C. All the above cell types can be cultured in vitro at their optimal growth condition including biological gas flow using nanoT - VaryCellTM .

Figure 2: nanoT – VaryCellTM
The design is compatible with any type of microscope such as inverted, upright, fluorescent, confocal, stereoscope as the footprint of the nanoT - VaryCellTM mimics a standard 96/ 24/ 24 tissue culture plate. nanoT - VaryCellTM is compatible with required biological gas supply such as 5 % CO2. MCF 7 cells were seeded on Day 0 and maintained within the nanoT - VaryCellTM up to Day 3 i.e. 72 hours at 37 °C, 5 % CO2, and 7.4 pH as shown in figure 3.

Figure 3: Time lapse imaging of MCF 7 cells maintained at 37 °C, 5 % CO2, and 7.4 pH for 3 days incubated within nanoT - VaryCellTM .

Feature that no other similar end time lapse imaging setups lack is time lapse imaging at cold

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