Class : FHSB1214 P16
Group Member : Lim Phei Ching Lee Jer Win Chong Hui Li

Title :
Investigation of Action of Saliva and 3 M Hydrochloric Acid in Two Carbohydrate Solutions .
Objective :
To Investigate of Effect of Salive and Hydrochloric Acid in Carbohydrate Solutions .
Apparatus & Equipment :
Boiling tubes , Beaker , Graduated plastic dropper , Water bath, ~ 37 oC , Water bath, ~ 95oC
Materials :
Carbohydrate solution A , Carbohydrate solution B , Benedict‟s solution , 3 M Hydrochloric acid , 3 M Sodium hydroxide , Iodine solution
Procedures :
Part 1
1. Two boiling tubes containing 1 ml solution A and 1 ml of solution B were prepared respectively . 1ml of Benedict solution was added into each tube . Both tubes were heated together in the 95°C water bath for two minutes . Results were recorded in table 1 .
2. A few drops of solution A and B were added separately on a white tile . 1-2 drops of iodine solution were added on each solution .
Part 2
3. Boiling tubes were labeled 1,2,3,4 . 2 ml of solution B was pipette into each of four boiling tubes .
4. Tubes 1 and 2 were placed in a water bath of 37°C to heat up the solution .
5. Small beaker was salivated till it reached about 5 ml .
6. This step was done approximately at the same time . 2ml of saliva prepared was pipetted into tubes 1 and 4 . The contents of tubes were shaken well to ensure through mixing . 2ml of HCL was pipetted into tubes 2 and 3 .
7. Tubes 1,2,3,4 were incubated at their respective temperatures for 30 minutes .
8. More new boiling tubes were labeled 1' 2' 3' 4'.
9. 2ml of the contents from all these tubes were poured out into the respective newly labeled tubes ( eg 1 into 1' , 2 into 2' etc ) after 5 minutes of incubation of tubes 1 to 4 .
10. The acid in each tube labeled 2' and 3'