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Mip Lab Report

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3. Results and discussion
3.1. Preparation conditions of molecularly imprinted polymer
The preparation process of MIP is quite simple, but a number of factors which affect the polymerization and imprinted effect have to be taken to account. For the synthesis of the MIP, MAA was chosen as the functional monomer because, from the previous experience, this is the best functional monomer in order to establish selective hydrogen bonding interactions with the functional groups of bentazon. Another important parameter that affected the capability of MIP synthesized in the synthesis process is molar ratio of template/monomer/cross-linker. Only appropriate molar ratio of template/monomer can afford high selectivity with MIP. And the amount of cross-linker should be high enough to maintain the stability of the recognition sites (Andersson et al, 1999). Through complex optimization experiments, the MIP …show more content…
4. Effect of pH on sorption of bentazon on imprinted polymer particles.
In order to investigate the optimum amount of MIP on the quantitative extraction of bentazon, the extraction was conducted by varying the amount of the MIP from 50 to 200 mg. The extraction of bentazon is quantitative by using 100 mg of MIP.

3.3. MISPE procedure of spiked surface water
In order to evaluate the applicability of the optimized MISPE procedure for trace analysis of bentazon existing in real samples, the procedure was applied for analysis of surface water spiked to the final concentration of 1 ng/mL (sample volume: 500 mL), 50, 100, and 200 ng/mL (sample volume: 20 mL). The obtained results from MISPE of surface water spiked with different amounts of bentazon are summarized in Table 1.

Table 1: The percentage recovery of bentazon on MISPE and NISPE of surface water spiked with bentazon, (n=5)
Analyte concentration (ng/ml) 1 50 100 200
Recovery on MISPE 91.2±2.4 92.3±3.6 92.8±1.5 94.1±2.6
Recovery on NISPE N.D. N.D. 52.6±1.8 65.5±2.3
N.D: Bentazon was not detected in elution

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