Molecular analysis of Jawahar Rice Hybrids for genetic purity and fingerprinting

Amrita Ingole,

Abstract

Varietal identification and genetic purity has attained critical importance worldwide especially in the context of plant variety protection. The estimation of hybrid seed purity is done conventionally by grow out test, which is based on the assessment of morphological and floral traits in plants grown to maturity, which locks up the capital while awaiting the results for GOT. GOT could be replaced with DNA based assays. The present investigation was undertaken with an objective to identify distinguishable microsatellite markers to establish fingerprinting of rice (Oryza sativa L.) hybrids, assessing variation within parental lines and testing the genetic purity of hybrid seed develpod by JNKVV Jabalpur, 4 cms, 4 restores and 5 hybrids were employed in this study. About 52 most informative microsatellite markers were employed for fingerprinting five rice hybrids and their parental lines. A total of 77 alleles were detected, and the number of alleles per locus ranged from 1 to 6, with an average of 2.56±1.33 primer pair. Eleven markers amplified specific alleles those separated the male from female lines. 13 markers were found polymorphic for female lines. Marker RM 164 clearly differentiates all the 4 CMS lines under study. 24 markers were found polymorphic among the male parents. No such marker was found that could differentiate all the male lines from each other. Polymorphic marker between the male and the female parent of respective hybrid were used for hybridity and genetic purity testing.SSR marker combinations that were unique to a particular parental or hybrid were identified which can detect impurities in seed lots of the hybrids. Key words: micro-satellite markers, genetic purity, Fingerprinting, GOT, rice hybrids

Introduction

Rice