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Observations to the Characteristics of Diffusion

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Observations on the Characteristics of Diffusion

A Scientific Paper

Submitted to Professor Christina Barazona
College of Science and Mathematics - Department of Biological Sciences
Mindanao State University – Iligan Institute of Technology
Andres Bonifacio Avenue, Tibanga, 9200 Iligan City, Philippines

By Janna R. Andalan

August 2013

ABSTRACT
The Experiment conducted involves diffusion. The point of this experiment was to know the characteristics of diffusion and to formulate hypothesis based on diffusion. It has also a purpose to see whether the diffusion is dependent on distance, rate and molecular weight of the substance. The estimation of distance is predicted by getting the average of the substance. Rate is predicted by subtracting the final diameter to initial diameter and dividing it by time.
It is determined that there are three things which influence the movement of molecules such as kinetic energy, nature of the environment and size of the molecules. By this, we know which dye diffused at the fastest rate by measuring the diameter of the colored area immediately after adding the substance to the agar plate. After one hour of measuring the methylene blue by 15 minutes interval, the substance moved immediately in 0min until 45minutes, the remaining minutes remains the same. While on the potassium permanganate, the substance moved from 0minutes to 1hour. These happened because they had different molecular weight and also the size of the substance that we put on the agar plate. It is expected that once a substance place into an agar plate it will move immediately because of the presence of the jelly in an agar.

INTRODUCTION

1.Diffusion is a process of equalization which involves movement of molecules from an area of high concentration to an area of low concentration. These molecules are in a constant state of motion and they are influenced by many factors such as kinetic energy, nature of the environment and size of the molecules. A series of observations will enable the student to derive some characteristics of diffusion.
2.Diffusion is one of several transport phenomena that occur in nature. A distinguishing feature of diffusion is that it results in mixing or mass transport, without requiring bulk motion. Thus, diffusion should not be confused with convection, or advection, which are other transport mechanisms that utilize bulk motion to move particles from one place to another. In Latin, "diffundere" means "to spread out".
3 Methylene Blue is a compound consisting of dark green crystals or crystalline powder, having a bronze-like luster. Solutions in water or alcohol have a deep blue color. Methylene blue is used as a bacteriologic stain and as an indicator. It inhibits GUANYLATE CYCLASE, and has been used to treat cyanide poisoning and to lower levels of METHEMOGLOBIN. Its Molecular Formula is C16H18N3SCl and 319.85222 g/mol its molecular weight.

4Potassium permanganate is an oxidizing agent with disinfectant, deodorizing and astringent properties. Its chemical formula is KMnO4 and it is sometimes called by its common name Condy's crystals and 158.034 g/mol its molecular weight. In its raw state potassium permanganate is an odorless dark purple or almost black crystal or granular powder. The main form of use is a potassium permanganate solution that is made by dissolving crystals or powder in water. There is also a more convenient 400mg tablet form of potassium permanganate available to prepare topical solutions. Crystals and tablets are available in pharmacies and garden shops.
Rate of Diffusion through a solution is the movement of high concentration of a molecule to lower concentration. The rate of diffusion depends on the concentration or molar mass of the molecule known and the rate of diffusion, the ration is known as Graham’s law of diffusion. The rate constant is a rate that factors in the correlation between viscosity and rate of diffusion.
The purpose of this experiment was to know the characteristics of diffusion and to formulate hypothesis based on diffusion, to see whether the diffusion is dependent on distance, rate and molecular weight of the substance.

MATERIALS AND METHODS

The Materials that I used in the experiments are marking pen for labeling the agar plates, scissors, masking tape, data notebook, tissue paper, liquid detergent, pencil and eraser, calculator, pencil ruler ( with calibration) 6 agar plates ,chemicals such as methylene blue ( powder )with plastic spoon and potassium permanganate ( powder )with plastic spoon.
The Procedure on this experiment was we obtained a pinch of potassium permanganate and methylene blue of about the same size. Then, place each substrate carefully on the center of the surface of the agar plate, carefully noting so that the crystals are spread equally over the agar and not too close to the edge of the dish. We made three replicates for each substrate and get the average value. Then, measure the diameter of the colored area immediately after adding the substance to the agar plate and record the measurement in millimeters (mm) for 0 minutes. In regular 15-mins interval, measure the diameter of the colored zone around each substrate and record both the time (in minutes) and diameters in Table 1. Continue measuring up to one hour. After gathering the data, compute the rate of diffusion for three substances using the formula: Rate = Final Diameter - Initial diameter Time
Then, plot the data on diffusion rate (mm/min) versus molecular weight (g/mole) on the graph provided on your answer sheet. Plot the data of the two substances ( methylene blue and potassium permanganate b to see any differences. Lastly, plot the data on distance (mm) versus time (min) on the graph provided on your answer sheet. Plot the data of the two substances (methylene blue and potassium permanganate) to see differences.

RESULTS & DISCUSSION

After one hour of observing there are many things that changed, from the time that we put a pinch of methylene blue and potassium permanganate until the time that we saw two substances move in each 15 minutes of intervals. The Diagram and table below shows different type of knowing the characteristics of the Diffusion rate of methylene blue and potassium permanganate.

Figure 1.0 Diffusion Rate between Methylene Blue and Potassium Permanganate

Methylene Blue(MW : 320 g/mol) | Potassium Permanganate(MW: 158 g/mol) | Time Distance Rate 0 10.33 0 15 13.67 0.22 35 14.67 0.14 45 15.67 0.12 60 15.67 0.09 | Time Distance Rate 0 7.33 0 15 17.67 0.69 35 25.33 0.6 45 29.33 0.49 60 31.33 0.4 |

The Table shows the relationship of potassium permanganate and methylene blue in its time, distance and rate.
Figure 1.1 Rate and Molecular weight of KMNO4 and C16H18N3SCl

The graph shows the rate of diffusion dependent to its molecular weight. It is shown that the smaller its molecular weight the bigger its diffusion rate. The Rate is predicted by using the formula of Rate which is Final diameter minus its Initial diameter and dividing it with time. By this formula we can obtain its result and can compare these two substances that the Potassium permanganate having the smaller molecular weight has a high diffusion rate its relationship is inversely proportional.

Figure 1.2 Distance and time rate between KMNO4 and C16H18N3SCl

The graph above shows that the distance and rate of each substance is dependent on the time the relationship between the diffusion rate and molecular weight is that the smaller the molecular weight the bigger the diffusion rate.
The Distance is predicted by getting its average value by adding the distance of the three replicates then we can obtain this result showing that as the time is moving the distance also move.

CONCLUSION

These Scientific paper means to derive the characteristics of diffusion, to formulate hypothesis based on the diffusion and to know the diffusion rate between the methylene blue and potassium permanganate. It also shows on this experiment that the diffusion rate between the methylene blue and potassium permanganate is dependent on the time, distance and molecular weight. The three things that influence the presence of the molecules are kinetic energy which due to the motion of the substance, nature of the environment which if the environment of the room is not that ventilated enough it may affect the result of your observation and time which is the most important to record the time of observation from 0minutes to one hour which we should pay attention to our substance so that we can know the difference from the start until we finish one hour of observing. The Potassium permanganate is the dye which diffused at the fastest rate due to it has the smaller molecular weight than methylene blue, from the time that we put the .potassium permanganate on the agar plate it moved immediately unlike with the methylene blue that it moved slower because it has a bigger molecular weight. Rate diffusion is dependent on molecular weight because if the molecular weight is not constant, then diffusion will vary independent on size. The relationship between diffusion rate is that the smaller the molecular weight the faster the diffusion rate. As the time of observing goes the distance of the substance also moved, but on the last 15 minutes the distance remains the same. Therefore, I conclude that the diffusion rate between the methylene blue and potassium permanganate is dependent on its molecular weight , distance of the substance that spread and on the time of observing its movement on the agar plate.

REFERENCES

1.Introduction to Biological Sciences BIO 101.1 Lab Manual 2.http://en.wikipedia.org/wiki/Diffusion
Osmosis/ Diffusion Questions ( Bio Team) 3.Vanessa Ngan, staff writer
Book: Textbook of Dermatology. Ed Rook A, Wilkinson DS, Ebling FJB, Champion RH, Burton JL. Fourth edition. Blackwell Scientific Publications.
Eczema. Starship Children’s Health Clinical Guideline http://www.dermnetnz.org/treatments/permanganate.html 4. National Center for Biotechnology Information, U.S. National Library of Medicine8600 Rockville Pike, BethesdaMD, 20894USA http://pubchem.ncbi.nlm.nih.gov/summary/summary.cgi?cid=6099 http://www.biology-online.org/biology-forum/about530.html Yahoo answers
http://answers.yahoo.com/question/index?qid=20101122094916AARurUs

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