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Temperature: Effects on Growth

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Temperature: Effects on Growth

Scientists have discovered that microorganisms grow on a broad temperature range from 0 degrees to more than 100 degrees. There are four groups that help classify their most favorable development. 1. Psychrophiles are when the most favorable development is between -5 degrees and 20 degrees. They are mostly found in artic areas. 2. Mesophiles are when the most favorable development is between 20 degrees and 50 degrees. This class is where most of the bacteria development occurs. 3. Thermophiles are when the most favorable development is between 50 degrees and 80 degrees. It is here that you ill find growth in compost piles where fermentation can occur. 4. Hyperthermophiles are when the most favorable development is when the temperature exceeds 80 degrees. They are mostly found in volcanic areas and hot springs. (Brown, 2007, p 181)
The microorganisms will grow in one of the four groups; they will not continue to develop into the other groups (Brown, 2007, p 181). Brown states that during this experiment we will attempt to measure the effects of various temperatures on pigment production and growth rate 2007, p 181). With this test there are three cellular components that are involved in metabolism that are influenced by temperature, they are cell membrane, ribosomes, and DNA. Those three cellular components are going to play a key role in the experiment. I believe that the Escherichia coli will have the greatest amount of growth out of the three bacteria we will be using for this experiment. Materials that will be needed are baskets that will be at different temperatures
(5, 25, 38, 42, 55 degrees), 6 test tubes of nutrient broth, wire loop, spectrophotometer, cuvettes, sterile nutrient broth and nutrient broth cultures of Serratia marcescens, Bacillus megaterium and Escherichia coli. There are

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