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The Growth and Characterisation of Bacterial Biofilm Underflow.

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Submitted By abhijeetb3
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MUTATION FREQUENCY OF PSEUDOMONAS AERUGINOSA(MPA01 AND PA1432) ,E.col,CRONOBACTER,in viable count(miles and misra) Planktonic Miles and Misra Date-15/07/2015 MPA01-10-6 PA1432-10-6 E.coli-10-6 CRONOBACTER-10-6 14 16 13 21 16 11 10 20 14 8 16 20 Average 14.66666667 Average 11.66666667 Average 13 Average 20.33333333 1466666667 1166666667 1300000000 2033333330

Date-16/07/2015 TSA+ciprofloxacin TSA+ciprofloxacin TSA+ciprofloxacin TSA+ciprofloxacin MPA01-100µl PA1432-100µl E.coli-25µl CRONOBACTER-25µl 141 42 2 9 167 84 2 7 156 65 4 18 180 24 10 32 124 38 17 17 sum 768 sum 253 sum 35 sum 83 MF 5.23636E-07 MF 2.16857E-07 MF 2.69231E-08 MF 4.08197E-08

MUTATION FREQUENCY OF PSEUDOMONAS AERUGINOSA(MPA01 AND PA1432) ,E.col,CRONOBACTER,in viable count(miles and misra) Planktonic Miles and Misra Date-23/07/2015 MPA01-10-6 PA1432-10-6 E.coli-10-6 CRONOBACTER-10-6 10 15 8 10 12 13 8 19 11 15 16 22 Average 11 Average 14.33333333 Average 10.66666667 Average 17 1100000000 1433333333 1066666667 1700000000 Date-24/07/2015 TSA+Rifampicin TSA+Rifampicin TSA+Rifampicin TSA+Rifampicin 6 5 24hrs-6 48hrs-4 10 24hrs-21 48hrs-15 36 2 10 24hrs-10 48hrs-2 12 24hrs-29 48hrs-17 46 3 8 24hrs-1 48hrs-4 5 24hrs-23 48hrs-13 36 3 12 24hrs-5 48hrs-2 7 24hrs-24 48hrs-15 39 7 11 24hrs-4 48hrs-0 4 24hrs-22 48hrs-10 32 Sum 21 Sum 46 Sum 38 Sum 189 MF 1.90909E-08 MF 3.2093E-08 MF 3.5625E-08 MF 1.11176E-07 Date-24/07/2015 TSA+ciprofloxacin TSA+ciprofloxacin TSA+ciprofloxacin TSA+ciprofloxacin 15 61 7 0 19 72 1 17 19 63 1 25 36 58 0 7 28 64 23 0 Sum 117 Sum 318 Sum 32 Sum 49 MF 1.06364E-07 MF 2.2186E-07 MF 3E-08 MF 2.88235E-08

MUTATION FREQUENCY OF PSEUDOMONAS AERUGINOSA(MPA01 AND PA1432) ,E.col,CRONOBACTER,in viable count(miles and misra) Planktonic Miles and Misra Date-25/07/2015 MPA01-10-6 PA1432-10-6 E.coli-10-6 CRONOBACTER-10-6 20 18 21 24 31 24 9 34 24 22 13 33 Average 25 Average 21.33333333 Average 14.33333333 Average 30.33333333 2500000000 2133333333 1433333333 3033333333 Date-26/07/2015 TSA+Rifampicin TSA+Rifampicin TSA+Rifampicin TSA+Rifampicin 12 17 24hrs-9 48hrs-13 21 24hrs-8 48hrs-5 13 16 19 24hrs-8 48hrs-14 22 24hrs-4 48hrs-4 8 19 16 24hrs-9 48hrs-4 13 24hrs-4 48hrs-4 8 6 18 24hrs-5 48hrs-19 24 24hrs-8 48hrs-5 13 7 Average 17.5 24hrs-6 48hrs-9 15 24hrs-6 48hrs-8 14 Sum 60 87.5 Sum 95 Sum 56 MF 0.000000024 MF 4.10156E-08 MF 6.62791E-08 MF 1.84615E-08 Date-26/07/2015 TSA+ciprofloxacin TSA+ciprofloxacin TSA+ciprofloxacin TSA+ciprofloxacin 196 134 0 5 218 179 1 8 182 176 0 4 246 178 0 1 207 204 5 8 Sum 1049 Sum 871 Sum 6 Sum 26 MF 4.196E-07 MF 4.08281E-07 MF 4.18605E-09 MF 8.57143E-09

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...Aacknowledgement First of all I like to thank God almighty who authorise me to research on this topic. I submit my sincere thanks to my supervisor Medhat Khattar for his valuable presence, time, effort, guidance and help to complete this dissertation. My dissertation would not have been completed without the help of lab technicians Nick and Suzy, I am extremely grateful for their help, suggestions and encouragement. I might want to thank my family for impacting in me a comprehension for the significance of education and an appreciation for diligent work. I extraordinarily value the majority of the penances that were made so as to realize the open doors that I have gotten, and it is my trusts that this proposition embodies what I have realized. Much obliged to you for your dedication, bolster, and affection. I might likewise want to thank my grandparents for the numerous hours of math mentoring as a youngster. In spite of the fact that it may have appeared to be inconsequential, it was the premise for my prosperity and the establishment of my hobbies in Designing. I might want to devote this proposition to my family, without whom I would not be seeking after a profession with an instruction from my university undergraduate days. I also thank my supervisor’s effort and good work channelled towards making me a better microbiologist in the world. I sincerely extend my thanks all concerned people who together with me in this regard.   Table of Contents I Declaration......

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