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The Hershey-Chase Experiment

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DNA and the importance of the Hershey-Chase experiment

Directions: Read text Ch. 10 pgs. 180-83 and then answer the questions below. Be prepared to discuss this in class.

1. What is molecular biology? What will you explore in this unit?
Molecular biology is the study of the molecular basis of genes and gene expression (molecular genetics).
We will explore the following:
-the structure of DNA
-how it replicates
-how it controls the cell by directing RNA and protein synthesis
-how it can change
-viruses that infect bacteria, animals, and plants
-bacterial genetics

2. Who are Hershey and Chase? What were they trying to figure out? Why were they trying to figure this out?
Alfred Hershey and Martha Chase were American biologists. They are trying …show more content…
coli (the host).
They used chemicals containing radioactive isotopes, a radioactivity detector, a kitchen blender, and a centrifuge.
The DNA and protein of the T2 were labeled using different radioactive isotopes. The DNA in a batch placed in a solution of radioactive phosphorus was labeled and the protein in another batch placed in a solution of radioactive sulfur was labeled.
They infected one batch of E. coli with the DNA labeled T2 and another batch of E. coli with the protein labeled T2. The two batches were placed into a blender (to separate the phages from the outside of the bacteria) and later spun in a centrifuge (to layer out so the bacteria form a pellet on the bottom). The radioactivity was later measured.

4. Explain what they found from the experiment (give details). Why was this important?
They found that the liquid in batch two (with the labeled proteins) had the radioactivity. That means that the proteins did not enter into the bacteria. However, in batch one (with the labeled DNA), the radioactivity was found in the pellet (the bacteria). This meant that the phage’s DNA entered into the bacteria. This is important because now we know that the DNA is what causes the host cells to produce phage DNA and proteins, which results in complete phages. We also know that the DNA contained instructions for making

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