...the appearance of certain volumes in pipet tips. Introduction: Micropipettes are equipment used to transfer and measure very small amounts of liquid as low as 0.003 ounce (0.1 millilitres) in laboratory. Standard micropipettes used in laboratory settings feature a few basic parts including an adjustment dial and a plunger button. Normally, micropipettes are consisting several of components, such as volume adjustment dial and a digital volume indicator. These pipettes also get used to different types of disposable tips at the end of a shaft which is one part of special of micropipettes. Liquids are drawn into the disposable plastic tips fitted at the end of micropipette shafts instead of into the shafts themselves. There are 2 main buttons found on micropipettes, which are the plunger button and the tip ejector button. The pipette will immediately release the disposable tip at the end of the device by pushing the ejector button. Most of plungers of micropipette are designed to stop at two different positions. Pipette will draw the desired volume of liquid up into its disposable tip by depressing the plunger to the first stopping position and slowly releasing it. A micropipette's plunger is then pressing down further to the second stopping point to release the volume of fluid drawn into at the first stop. For accuracy measurement, pipette plungers should be pressed down to first stop point before the pipette tip gets inserted into a sample. Volume...
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...Cook noodles until barely tender, about 2 minutes less than package directions. Drain, rinse and set aside. 3. Heat oil in the pot over medium heat. Add carrots, celery, onion and garlic and cook until beginning to soften, about 5 minutes. Add chicken and cook until no longer pink on the outside, 5 to 7 minutes. Whisk cornstarch and milk in a medium bowl; add to the pot along with salt. Bring to a boil over medium-high heat, stirring often, until bubbling and thick enough to coat the back of a spoon, about 4 minutes. Remove from the heat and stir in hot sauce. 4. Spread the noodles in a 9-by-13-inch (or similar 3-quart) baking dish. Top with the chicken mixture; sprinkle with blue cheese. 5. Bake the casserole until it is bubbling, about 30 minutes. Let stand for 10 minutes before serving. Tips & Notes * Make Ahead Tip: In Step 2, cook the noodles 4 minutes less than package...
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...proteins. Loading the GFP supernatant onto the chromatography column When students load the GFP supernatant onto their columns, it is very important that they do not disturb the upper surface of the column bed when performing the chromatography procedure. The column matrix should have a relatively flat upper surface. A slightly uneven column bed will not drastically affect the procedure. However, subsequent steps of loading, washing, and eluting should minimize disrupting the column such that beads "fluff up" into the buffer. When loading the GFP supernatant onto the column, the pipette tip should be inserted into the column and should rest against the side of the column. The supernatant should be slowly expelled from the pipette, down the walls of the column. When the supernatant has completely entered the column, a green ring of fluorescence should be visible at the top of the bed when viewed with the UV light. There are four different buffers which are used in the HIC procedure. Each buffer should be slowly pipetted down the side of the column to...
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...Use this cheat sheet of speed tips and mental cues for instant speed gains A faster athlete is a better athlete, but sprinting with perfect form can take years to master. The right coaching cue can instantly fix running form problems and will immediately make you faster. Check out these speed hacks that will unlock your inner-speed. www.stack.com/order/speed-kills WHEN TRAINING… 1 Chew On Some Gum Tip: Running with gum in your mouth will prevent you from tensing your jaw and will keep you more relaxed. 2 Pinch Your Pennies Tip: Pinching a penny between your thumb and the base of your index finger keeps your hands more relaxed for better arm drive. Benefit: By reducing tension in your jaw you’ll also loosen up your upper-body so you have a more fluid arm swing, stronger leg drive and greater speed. Benefit: Reducing tension in your arms and upper body will give you a smoother arm swing, which will improve your form and overall speed. www.stack.com/order/speed-kills WHEN ACCELERATING… 3 4 5 Downward Gaze Tip: Focus your eyes on the ground just a few feet in front of you. Benefit: This helps you stay low during your start to propel your body forward and not up. That produces more explosive and efficient acceleration to help you reach top speed faster. Break Some Wind Tip: Imagine you have to run through a 100 mph wind when accelerating. If you stand straight up the wind will blow you back. Get a good forward lean to cut through the wind. Benefit: By having the proper...
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...The Dos and Don’ts of Effective Business Communication in Today’s World Business communication takes different forms including verbal, oral and electronic. It is essential for the success of every company and organization nowadays as it influences its managing and performance. (Vitez, 2014) Therefore there are main dos and don’ts that everybody from employees to top managers should know in order to communicate efficiently. To begin with, one should know what to do when communicating in a business environment. There are main things to be considered before starting a communication. Firstly, the receiver of the information should be identified. It allows the sender to understand how to gain attention and make the information understandable and appropriate. For example, an email written for the top manager has to be different than one for an intern as they have different knowledge and interests. The next step is to know what is the main message and its aim. Knowing what the sender wants to say to the auience and why allows him to structure the information in a clear way and stay focused. (“10 Tips For Effective Business Writing”, n.d.) The formulation of this information also affects its effeciveness. The best thing to do is to begin with the main message. (O'Hara, 2014) It helps the receiver to understand quickly the reason behind it and to grab the attention. When communicating through writing gramatical and speling mistakes might occure. Therefore, the sender should check...
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...| |Your Guide for Travel and Hotels in Columbia | |When you hear the word vacation, what do you see in your mind? Vacations mean assorted things to assorted individuals. Some like to sight see while many others would | |enjoy nothing more than to spend the whole trip kicking back at the hotel pool. If you happen to be like me, you enjoy some of both options. On the one hand, because I| |have journeyed someplace different, I ought to visit some interesting local fare. But then again, if I use up all my time darting around from site to site, when I come| |home I will want another vacation to recuperate. To get the most from your vacation time, keep the following in mind when travelling to Columbia. | | | |The first thing you ought to do is resolve as much at the start as you can. That means things like interchanging currency when necessary, glancing at maps of the area,| |renting a means of transportation (did someone say Harley?), booking a flight, and selecting a hotel that fulfills your needs. The good news is, all of these jobs have| |become much easier and cheaper through the internet, so before contacting a travel agent, you could save some money by looking up...
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...HTML Tags Chart To use any of the following HTML tags, simply select the HTML code you'd like and copy and paste it into your web page. Tag | Name | Code Example | Browser View | <!-- | comment | <!--This can be viewed in the HTML part of a document--> | Nothing will show (Tip) | <a - | anchor | <a href="http://www.domain.com/"> Visit Our Site</a> | Visit Our Site (Tip) | <b> | bold | <b>Example</b> | Example | <big> | big (text) | <big>Example</big> | Example (Tip) | <body> | body of HTML document | <body>The content of your HTML page</body> | Contents of your web page (Tip) | <br> | line break | The contents of your page<br>The contents of your page | The contents of your web page The contents of your web page | <center> | center | <center>This will center your contents</center> | This will center your contents | <dd> | definition description | <dl> <dt>Definition Term</dt> <dd>Definition of the term</dd> <dt>Definition Term</dt> <dd>Definition of the term</dd> </dl> | Definition Term Definition of the term Definition Term Definition of the term | <dl> | definition list | <dl> <dt>Definition Term</dt> <dd>Definition of the term</dd> <dt>Definition Term</dt> <dd>Definition of the term</dd> </dl> | Definition...
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...If you cannot find almond flour where you live, check out this great video from my new friend Dzung at Honeysuckle Catering and she'll show you a great easy way to make it yourself!http://bit.ly/WZQ1fZ RASPBERRY BUTTER CREAM ½ cup salted butter (120 g) 3/4 cup powdered sugar (75 g) 1 cup (150 g) fresh raspberries, worked through a sieve to extract 3 tbsp of juice METHOD: Preheat oven to 300 degrees Beat egg whites and sugar for 8-10 mins. TIP: #1 eggwhite should be room temp. To create room temp eggs, submerge in warm water for 5 mins. Whip until they form a peak that stands upright. Think Seattle Space needle. Then add the food coloring. TIP#2 Color does fade as it cooks, so do a shade or two darker than you want them to be. TIP#3 Sift almond flour, and powdered sugar. What remains will be the larger lumps of almond pieces. Just discard those, or use them to snack on :) You want a really fine powder mixture to create a smooth and pretty on top to your cookie. Fold flour/sugar mixture into the egg white mixture. TIP#4 This is where all your hard work can really go wrong. Under mix and your macaroons will be lumpy and cracked when the bake with no feet, over mix and your macaroons will be flat and won't have feet, the mark of a well-made macaron. In my experience 65-75 turns of your spatula when folding is about the right amount of time. But again, it can be tricky, depends on how strong you are, Ha! So it can take a few tries to get it right. But when you...
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...10 Steps to Earn a High GRE Score The following is a guide to scoring high on the GRE test. If you follow these steps, your GRE score will improve. 1. Relax: Preparing for the GRE can be stressful. Try to focus on the task at hand and not so much on everything else you have to do. If you take GRE test prep step-by-step, day-by-day, and give yourself breaks when your body needs them, you'll be able to concentrate on GRE studies a lot better. 2. GRE Diagnostic Test: Begin GRE test prep with a GRE practice test to find out what you're good at, and more importantly, what you're not good at. Correct your completed practice test and evaluate the questions you missed. What kind of questions did you miss? Are they mostly Quantitative or mostly Verbal? What score would you give yourself on Analytical Writing? Did you miss a lot of analogies but do well on reading comprehension? With this information you'll be able to focus your GRE test prep studies on your weaknesses. 3. GRE Study Plan: With your GRE weaknesses in mind, make a very specific study plan for yourself. You should know what, how, and when you are going to study for the GRE test. Put your study plan somewhere in plain view, and make sure you stick to your plan. Try to keep GRE test prep interesting by planning to study different things in different ways. For example, study GRE vocabulary from flash cards one day, from a list the second day, and by reading and learning words in context the third day. 4. GRE Vocabulary: ...
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...HTML Tags Chart To use any of the following HTML tags, simply select the HTML code you'd like and copy and paste it into your web page. Tag | Name | Code Example | Browser View | <!-- | comment | <!--This can be viewed in the HTML part of a document--> | Nothing will show (Tip) | <a - | anchor | <a href="http://www.domain.com/"> Visit Our Site</a> | Visit Our Site (Tip) | <b> | bold | <b>Example</b> | Example | <big> | big (text) | <big>Example</big> | Example (Tip) | <body> | body of HTML document | <body>The content of your HTML page</body> | Contents of your web page (Tip) | <br> | line break | The contents of your page<br>The contents of your page | The contents of your web page The contents of your web page | <center> | center | <center>This will center your contents</center> | This will center your contents | <dd> | definition description | <dl> <dt>Definition Term</dt> <dd>Definition of the term</dd> <dt>Definition Term</dt> <dd>Definition of the term</dd> </dl> | Definition TermDefinition of the termDefinition TermDefinition of the term | <dl> | definition list | <dl> <dt>Definition Term</dt> <dd>Definition of the term</dd> <dt>Definition Term</dt> <dd>Definition of the term</dd> </dl> | Definition TermDefinition...
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...TIP TOP MARKETS CASE Moldoveanu Florentina Tip Top Markets is a regional chain of supermarkets located in the Southeastern United States. Every Tuesday for nine weeks, Tip Top Markets diligently recorded all of the complaints or comments sent in by customers. There were a total of 184 complaints received during the past 9 Tuesdays. As you can see into the chart below, the number of complaints received every Tuesday are moving horizontally with no indication of either going up or down in the coming Tuesdays. They just fluctuate about the average which is 20. The same level of complaints will continue in the future until more focused and effective strategies are implemented. [pic] I split all the complaints – 184, in 12 categories: ✓ Out-of-stock - 61 ✓ Overcharged - 18 ✓ Poor store maintenance – 16 ✓ Long check-out waiting time – 14 ✓ Defective products – 12 ✓ Uncomfortable store temperature – 10 ✓ Meat not fresh – 9 ✓ Wrong price/under charge – 8 ✓ Could not find items – 8 ✓ Employees not courteous/helpful – 6 ✓ Fish not fresh – 5 ✓ Others – 17 The category Out Of Stock has the most number of complaints - 61 and the category Fish Not Fresh has the least number of complaints - 5. The category Others includes all other complaints that minor complaints like: ATM ate card, lost wallet, dislike store decoration, etc. The problems that I found in this case are the following: ...
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...Baking Terms Allumette: Any of various puff pastry items made in thin sticks or strips (French word for "matchstick"). Almond Paste; A mixture of finely ground almonds and sugar. Angel Food Cake: A type of cake made of meringue (egg whites and sugar) and flour. Angel Food Method: A cake mixing method involving folding a mixture of flour and sugar into a meringue. Baba: A type of yeast bread or cake that is soaked in syrup. Babka: A type of sweet yeast bread or coffee cake. Baked Alaska: A dessert consisting of ice cream on a sponge-cake base, covered with meringue and browned in the oven. Baking Ammonia: A leavening ingredient that releases ammonia gas and carbon dioxide. Baklava: A Greek or Middle Eastern dessert made of nuts and phyllo dough and soaked with syrup. Batter: A semiliquid mixture containing flour or other starch, used for the production of such products as cakes and breads and for coating products to be deep-fried. Bavarian Cream: A light, cold dessert made of gelatin, whipped cream, and custard sauce or fruit. Beignet Soufflé (ben yay soo flay): A type of fritter made with éclair paste, which puffs up greatly when fried. Blanc Mange (bla mahnge): (1) An English pudding made of milk, sugar, and cornstarch. (2) A French dessert made of milk, cream, almonds, and gelatin. Bloom: A whitish coating on chocolate, caused by separated cocoa butter. Blown Sugar: Pulled sugar that is made into thin-walled, hollow shapes by being blown up like a balloon. Bombe:...
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...mobile app users in an effort to understand the successes and challenges developers face when vying for the top spot in various app stores. The results are useful as you develop your plan to market mobile apps. This month’s column will cover the results of my company’s B2B mobile application research. The results of the B2C mobile application research will be covered in my next column. Purpose The purpose of the application research was to uncover app marketing techniques employed to drive mobile app discovery and downloads of native apps developed for the top mobile platforms. Criteria A respondent was required to be a developer of native mobile apps for iPhone, iPod Touch, Android, and BlackBerry devices. Each of these platforms offer a proprietary “app store” to developers for managing the discovery, download, and monetization of apps developed on their respective platforms. Most respondents to our survey were iPhone app developers (53 percent) that develop native iPhone apps as their primary business (47 percent). App Store Rankings Tip: Learn how rankings in each app store work and how they affect the number of installs an app receives. Many of the top apps are using mobile app advertising campaigns to get them to the top of the ranking. Try flighting – run promotion to get to the top and then stop promotion and let it ride until the app drops below the top rankings, then run promotion again. Other app stores to consider are: Handmark,Handango, GetJar, and...
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...*Sizes & dimensions are approximate. Ring Sizing Guide Method A: Measure Your Finger Make sure your printersizer.to 100-percent. Do not reduce the print size in the print ‘page 1. Make sure your printer is set to 100 percent. Do not reduce your printer size in your page setup area (see dialog box below). Cut out the ring is set setup’ area. You can verify thatsure this page is printed at the correctwith a ruler; it should Use a quarter to make the ring sizer printed correctly by measuring it size. 2. Cut a3slit for Slot "A". "A" to "B". measure 1/2 inches long from 3.The printed quarter should finger where the ring an actual quarter placedthe numbers are facing out. Wrap the sizer around the be the same size as will be worn. Make sure on top of it. To open the print dialog box, press CTRL + P for pc or COMMAND +P for mac. 4. Pull the pointed end Tab "B" through Slot "A"until the sizer is snug. Make sure to allow size for your knuckle. If your To open the in between the press knuckle is sizeprint dialog box,two. CTRL+P for PC or COMMAND+P for Mac. 5. The number aligned with Slot "A" is your ring size. Use a quarter to 6. If the measurement lands between two numbers, your size is a half size. make sure this page Example: IfSample Dialog Box between 5 and 6, your ring size is 5 1/2. your measurement falls is printed at the correct size. Use a quarter to make sure this page is printed at the correct size. 1. Cut out the ring sizer. finger at the beginning of the day...
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...eNewsletters Resource Center Buyers' Guide Webcasts Events Blogs News About Us Contact Us Advertise Privacy Policy Press Room Reprints Feedback Employee Benefits Network: BenefitsPro FreeERISA Benefits Selling Broker Innovation Lab Benefits Selling Expo eNewsletters Magazine Feedback? Search Twitter Facebook Google+ LinkedIn Read Next Article Best sales and marketing tips: Social media Share This Story Advertisement Home / Benefits Brokers / Sales Strategies Best sales and marketing tips: Old school 100 sales and marketing tips, part 2 Jan 06, 2015 | By Kathryn Mayer Email LinkedIn Twitter Facebook Google+ Share This Story (Image courtesy of ponsulak/FreeDigitalPhotos.net) (Image courtesy of ponsulak/FreeDigitalPhotos.net) Our 100 sales and marketing tips list this year is broken down into 10 important categories. We've already covered social media. This list focuses on old school sales techniques. 11. Forget the group meetings, the conference calls, the social media posts: Meet with clients one-on-one. They still like it. 12. Send cards to your clients for birthdays, anniversaries or Christmas (via snail mail, not just email). Advertisement 13. Always carry business cards with you (and give them out!) 14. Smile. Make eye contact. You know, be a friendly person. 15. Get up early...
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