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Translation Lab

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Submitted By baseball19
Words 2429
Pages 10
Introduction:
Translation Lab will allow you to study the importance of the nucleotide sequence of mRNA as the fundamental basis for the genetic code universally deciphered by living cells. You will produce sequences of ribonucleotides that will be translated into protein to simulate the landmark experiments involving cell-free extracts that were essential for interpreting and understanding the genetic code. A major step forward in figuring out the code was the discovery by Nirenberg in 1961 that a cell-free extract made from E. coli cells could translate RNA added to the extract into proteins. The composition of the newly synthesized proteins could be determined by measuring the incorporation of radioactive amino acids into these proteins as they were translated. In his first experiment he made poly U RNA, using the enzyme polynucleotide phosphorylase, and translated it into a peptide of polyphenylalanine using the cell-free extract. This was definitive proof that RNA could code for the synthesis of proteins and gave the first possible assignment of a nucleotide code to the amino acid it specified.

Methods and Materials:
For each of the four bottles of ribonucleotides that appear, click on the arrow to select a nucleotide. Do this for two nucleotides initially. Click the Make RNA button to display the sequence of mRNA that you created. Click Add to Notes to create a record of your experiment. To translate this sequence into amino acids, click on the To Translation Mix button. Click Add to Notes to add the amino acid sequence to your notebook. Some materials used for this experiment are Protein sequencer, Nucleotides, Translation mix, RNA Synthesizer, and a Lab notebook. * Nucleotides * RNA synthesizer * Translation mix * Protein sequencer * Lab notebook
Results:
RNA: GCGCGCGCGCGCGCGCGCGCGCGCGCGCGC...
Peptide Sequences:

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