...Information 2 2. Safety Information 2 3. Attendance 3 Make-Up Policy 3 4. Laboratory Protocol 3 Assigned Reading 3 Pre-Lab Quizzes 3 Lab Notebook 5 Chemicals 5 Due Dates for Reports 5 5. Orientation 5 In-Lab Information 5 Library Information 5 6. Check-In 6 7. Grading Procedure 6 8. Policy on Cheating 7 9. TA Office Hours 8 10. Faculty Course CoordinatorS 8 11. Course Web Page 8 12. Hints to Minimize Frustration IN ORGANIC CHEMISTRY 8 13. Work Schedule 10 Lab Report Due Date Schedule 10 Experiments 10 14. Supplements 17 A. Extraction of Unknown 17 B. Recrystallization of Unknown Products 18 C. Methyl Benzoate 19 D. Synthesis of Luminol 20 E. Azo Violet 23 1. GENERAL INFORMATION PRE- and CO-REQUISITES Pre- and co-requisites for CH 220C listed in the Course Schedule. Important: Because the lecture and laboratory courses are co-requisites of each other, dropping one of them requires that you drop the other as well, unless the drop occurs during the final 2 laboratory periods of the term. Pre- and co-requisites will be checked and students not meeting...
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...4/15/2015 BIO156 Lab 2 Print Lab 2 Biological Molecules and pH Introduction: Connecting Your Learning Biological organisms, like all things, are made up of elements. These elements combine to form organic molecules that create the basis for life. The main elements found in biological organisms include carbon (C), hydrogen (H), phosphorus (P), nitrogen (N), and oxygen (O). This lab describes how these elements form some of the most important molecules in life: carbohydrates, proteins, and fats. Resources and Assignments Multimedia Resources Required Assignments None Lesson 2 Lab 2 From the Lab Kit 7 test tubes Benedict's solution Biuret solution 15 micropipettes 10 pipettes Forceps pH test strips 4 unknown samples https://www.riolearn.org/content/bio/BIO156/BIO156_INTER_0000_v9/labs/lab02.shtml?print 1/21 4/15/2015 BIO156 Lab 2 Measuring spoons (teaspoon and tablespoon) 50 mL beaker Mortar and pestle Glass stirring rod 100 mL graduated cylinder Microscope slide Plastic funnel Test tube tongs Test tube rack 5 plastic cups Goggles Plastic gloves 1 tablespoon baking soda 1 tablespoon chicken soup 4 tablespoons sugar Required Materials 1 tablespoon cornstarch 2 tablespoons unflavored gelatin Student Provided Small saucepan Paper towel Oven glove or mitt Baking tray or aluminum foil (about an 18-inch sheet) Scissors Pencil Dime Microwave (optional) or Stove Permanent marker https://www.riolearn...
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...liquid hypochlorite household bleach. There are two oxidation-reduction reactions for determining the oxidizing capacity of bleach. Initially an excess of iodide ions are added to a bleach solution. The iodide ions are oxidized to iodine after the solution has been acidified. Starch is added to the resulting iodine solution as an indicator. The solution is then titrated with sodium thiosulfate until the color of the solution changes indicating the endpoint of the reaction. Data collected from the titrations will be used to calculate the mass of the sodium hypochlorite in an unknown solution of bleach. Given the original mass of the unknown solution and the calculated mass of the sodium hypochlorite in the unknown solution, the percent by mass of the sodium hypochlorite can be determined. The oxidizing capacity of the unknown bleach is effectively the percent by mass of the sodium hypochlorite in the unknown bleach sample. OJECTIVES 1) Titration of a sample of bleach with thiosulfate. 2) Determining the end point of the titration with starch indicator. 3) To determine oxidizing capacity of bleach by determining the percentage by mass of the bleach that is sodium hypochlorite. 4) Balance and summarize the two redox reactions to determine the overall chemical reaction that occurs during the titration. PRINCIPLES Oxidizing Capacity of Bleach The effectiveness of a bleach to whiten and remove stains in white clothing is related to the oxidizing (bleaching) strength of an oxidizing...
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...METHODS Student Number and Name:1316740 Katerina Koupa | Name of Lab Instructor: Dr. J. Walton | Date of the experiment:06/02/2014 | Date of Report Submission:20/02/2014 | (Fig.1) Biuret Reagent Structure ABSTRACT: This report describes the use of the Biuret and Folin methods of protein assay, to verify the protein concentrations of two unknown solutions. To calculate the protein concentrations of the unknowns, nineteen samples were passed through a spectrophotometer which twelve of them had a known protein concentration and there absorbance levels were found. Then a calibration graph was plot that determines the concentration levels of the unknown samples. INTRODUCTION: One of the most typical procedures applied by lab scientist is the protein quantification. Finding protein concentrations in solutions is important in many ways. First of all detecting the levels of a protein in body fluids can result in identifying various diseases. Also protein verification is required for characterization and purification of enzymes. Because of the significance of protein assays laboratories perform this techniques on almost a daily basis. For this experimental procedure, the Biuret and Folin methods were used to determine the protein concentrations of two unknown samples. First the Biuret method (Fig.1) is based on the appearance of peptides bonds in proteins. When cupric ions (Cu2+) come in contact with a compound of proteins in a quite...
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...Attendance, P4 Lab Schedule, P4 Lab Replacement, P5 Lab Regulation 6.1 General Rules, P5 6.2 Safety Rules, P6 Lab Rules & Regulations on Computer Usage 7.1 ICT Computer Lab, P7 7.2 ICT Internet Lab, P8 Appendix 1 (Lab Replacement Flow Chart), P10 Appendix 2 (Lab Session Replacement Form), P11 Policy for Laboratory Usage after Office Hours, 10.1 Operating Procedure, P12 10.2 Warnings on liability, P12 10.3 Rules and Regulations, P12 Use of Laboratory After Office Hours 11.1 Appendix 3 (Application Form), P14 11.2 Appendix 4 (Student’s Declaration Form), P15 Ambulance Services, P16 Lab Safety Handbook on Chemical Hazards, Physical Hazards and Biological hazards, P17-P64 7 8 9 10 11 13 14 Universiti Tunku Abdul Rahman 2 Definitions • • • • • Lab Session: Time duration allocated for student to do lab experiment. Lab Sheet: A printed material usually contains a series of instructions and information given to the student on how to conduct lab experiment. Lab Report: A written report prepared by student based on individual observation and data analysis after the lab experiment. The format and requirements are usually stated in the lab sheet. Lab Coordinator: A person in charge of coordinating all the lab sessions of the semester and administrating lab matters. Lab Instructor: An academic staff (lecturer or tutor) in charge of the lab session. The lab instructor will give briefing and instructions to students during the lab session. 1. Introduction Practical lab is one...
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...http://www.labcompliance.com/tutorial/methods/default.aspx?sm=d_d TutorialHome | Contact Us | Newsletter | Usersclub | Books | Audio Seminars Seminars Audio Seminars Video Seminars Workshops Literature Books SOPs Validation Examples Free Literature Glossary Usersclub Intro Log-in Register Preview Renewal Tutorials Risk Management Practices Computer Validation Part11 Method Validation ISO 17025 Lab Equipment Qualification Good Laboratory Practices About About Labcompliance Contact Labcompliance Scope Tax/Bank Information All come with 10+ Best Practice Documents: SOPs, Checklists, Examples Transfer of Analytical Procedures According to the New USP Chapter <1224> With SOPs, templates and examples for easy implementation March 21, 2013 Quality by Design (QbD) for Analytical Method Development and Validation Learn how to design robustness for easy transfer and to avoid OOS situations Recorded, available at any time Validation of Analytical Methods for GLP and Clinical Studies Learn how to design, prepare, conduct and document for FDA compliance Recorded, available at any time Eight Steps for Cost-effective Laboratory Compliance Up-to-date overview, hot topics and trends. Recorded, available at any time Verification of Compendial Methods according to the New USP Chapter <1226> Understand the new risk based approach and and get real world case studies for testing Recorded, available...
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...information on accurate drug testing, and the types of drugs that are tested in the workplace. BBB wants information regarding if the drug tests are just as accurate with all drugs tested for. The Better Business Bureau uses the information provided to grade a business. They determine if the drug testing companies meet Better Business Bureau accreditation standards. The Better Business Bureau is a nonprofit organization that was founded in 1912. It exists to gather and review information on businesses based on the information, and consumer reports that the BBB has received, and give the business a grade. The Better Business Bureau grades businesses with letter grades from A+ (highest) to F (lowest). The BBB will usually explain the major reasons that raised or lowered a business’ grade. The consumers then utilize this information to decide if they want to do business with a certain business. Consumers are also the ones who generally file reports, and complaints to the Better Business Bureau about a business. The goal for the Better Business Bureau is to review drug testing companies and to give them a grade for consumers, such as business’ that are on the market for a drug screening company. The Better Business Bureau wants information on which drugs are tested for, and how accurate those drug tests are. The BBB is...
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...Studies VII Practical 9 Cell Biology Studies IX Practical 10 Cell Biology Studies X - Experiment Description Page Writing of Lab Reports Identification of Biomolecules 5 13 Identification of Unknown Carbohydrate Solutions and Investigation of Action of Saliva and HCl in Carbohydrate Solution at Two Different Temperatures Investigation of the Effects of Catalase Concentration on Hydrogen Peroxide Decomposition 20 Synthesis of Starch Using an Enzyme Extracted from Potato Tuber Investigation of the Effects of Different Catalytic Conditions on Hydrogen Peroxide Decomposition Microscopy 27 Practical 6 Cell studies II Practical 7 Cell studies III Extraction of Cell Organelles by Cell Fractionation Determination of Solute Potential of Potato Cell Sap 47 Practical 8 Cell studies IV Effects of Different Treatments on Stained Potato Cells 64 Practical 9 Energetics I Respiration of Germinating Beans 67 Microscopic Examination of Cells at Various Stages of Plant Mitosis and Meiosis DNA, Mitosis and Meiosis Modelling 71 Respiration of Yeast 93 Practical 3 Enzyme studies I (Experiment 1) Optional: Practical 3 Enzyme studies I (Experiment 2) Practical 4 Enzyme studies II Practical 5 Cell studies I - - Practical 10 Energetics II Lab manual version 6_201505 FHSB1214 Biology I & FHSC1214 Fundamentals of Cell...
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...Studies VII Practical 9 Cell Biology Studies IX Practical 10 Cell Biology Studies X - Experiment Description Page Writing of Lab Reports Identification of Biomolecules 5 13 Identification of Unknown Carbohydrate Solutions and Investigation of Action of Saliva and HCl in Carbohydrate Solution at Two Different Temperatures Investigation of the Effects of Catalase Concentration on Hydrogen Peroxide Decomposition 20 Synthesis of Starch Using an Enzyme Extracted from Potato Tuber Investigation of the Effects of Different Catalytic Conditions on Hydrogen Peroxide Decomposition Microscopy 27 Practical 6 Cell studies II Practical 7 Cell studies III Extraction of Cell Organelles by Cell Fractionation Determination of Solute Potential of Potato Cell Sap 47 Practical 8 Cell studies IV Effects of Different Treatments on Stained Potato Cells 64 Practical 9 Energetics I Respiration of Germinating Beans 67 Microscopic Examination of Cells at Various Stages of Plant Mitosis and Meiosis DNA, Mitosis and Meiosis Modelling 71 Respiration of Yeast 93 Practical 3 Enzyme studies I (Experiment 1) Optional: Practical 3 Enzyme studies I (Experiment 2) Practical 4 Enzyme studies II Practical 5 Cell studies I - - Practical 10 Energetics II Lab manual version 6_201505 FHSB1214 Biology I & FHSC1214 Fundamentals of Cell...
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...------------------------------------------------- Antimicrobial Susceptibility, MIC and MBC ------------------------------------------------- Microbiology Lab Report 3 Carolina Toscanojara Antibiotic Sensitivity. Objective: Antibiotics are chemical compounds that selectively interfere with the growth of microorganisms while causing little or no damage to the animal or human host. The purpose of this lab was to determine the effectiveness antibiotics against any given bacterium by means of examining the zone of inhibition, the minimum inhibitory concentration, and the minimum bactericidal concentration. Introduction: Chemical antimicrobial agents are chemical compounds that are capable of either preventing the microorganisms for multiplying (bacteriostatic) in order for the immune system of the host to overcome them or killing them completely (bactericidal). Antibiotics taken to improve the symptoms from a disease are called chemotherapeutic drugs; they are produced by a microorganism such as bacterium or fungus and/or are derived from a chemical produced by one. The test carried out to determine which antibiotic will be most successful in treating certain bacteria is called Antibiotic Susceptibility Test (AST) and it is often done by the Kirby-Bauer method, which uses Muller Hinton agar and paper discs containing specific concentrations of antibiotics are placed on the agar surface after rubbed with bacterial specimen. Results of AST are examined for zones of inhibition...
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...LAB REPORT BLOODSTAIN ANALYSIS (http://www.crimescene-forensics.com/images/Blood_Spatter_001B.gif) COURSE: CHM 1107: Introduction to Forensic Science - Professor Ira S. Krull DATE: 10.27.11 INTRODUCTION Blood is a biological fluid found in animals that delivers necessary nutrients and oxygen throughout the body and carries away metabolic waste products. The average adult has a blood volume of roughly 5 liters, comprising of plasma, blood cells and platelets, and it is usually red in color, though not always. The most abundant cells in vertebrate blood are red blood cells, constituting about 45% of whole blood. These cells contain hemoglobin, an iron-containing protein, which distributes oxygen throughout the human body. Blood is circulated around the body through blood vessels by the pumping action of the heart. In humans, blood is pumped from the strong left ventricle of the heart through arteries to peripheral tissues and returns to the right atrium of the heart through the superior vena cava (see Fig 1). It then enters the right ventricle and is pumped through the pulmonary artery to the lungs and returns to the left atrium through the pulmonary veins. Blood then enters the left ventricle to be circulated again. (Fig 1: http://upload.wikimedia.org/wikipedia/commons/thumb/e/e5/Diagram_of_the_human_heart_%28cropped%29.svg/220px-Diagram_of_the_human_heart_%28cropped%29.svg.png) Blood is a type of evidence found most often at violent crime scenes and...
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...on last minute revision for tests and examinations, as it will be too late to catch up and seek help in areas where you may find confusing or unclear of. • Why panic before exams because you can’t find this or that? Keep separate files for lecture, tutorial and practical. File up the respective notes systematically so that you do not lose them along the semester. • Do you expect the lecturer/ tutor to be available all the time to answer your questions? It is YOUR responsibility to take the initiative to clear your doubts or satisfy your curiosity to understand certain scientific phenomena by reading up on the relevant topics. A Based on a true story… A professor at the National University of Singapore recounts how on one occasion a student consulted him days before the exam. Student: Prof, could you explain this page to me please? Professor: What don’t you understand about this page? Student: EVERYTHING. Professor: But I already went through this during lecture. Student: Oh, I didn’t attend most of the lectures actually. As for the next page, could you explain this page to me please?... and this page too… and that too… Prof: I’m sorry, I can’t help you....
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...exposure. Human beings in such remote locations as Canada’s far northern Baffin Island now carry traces of persistent chemicals in their bodies, including notorious compounds as PCBs, DDT and dioxin. Even worse, in the womb and through breast milk, mothers pass this chemical legacy on to the next generation. “Our Stolen Future”, the scientific discovery of Theo Colborn, Dianne Dumanoski and John Peterson Myers, takes up where Carson left off and reviews a large and growing body of scientific evidence, linking synthetic chemicals to aberrant sexual development and behavioral and reproductive problems, such as low sperm counts, infertility, genital deformities, hormonally triggered human cancers, like those of breast and prostate gland, neurological disorders in children such as hyperactivity and deficits in attention. The quality of men's sperm declined steadily in the early years of the 21st century until hardly anyone could reproduce in the normal way. Meanwhile, the countryside was virtually emptied of animals as natural populations crashed. The first signs of the impending catastrophe were noted in the 1990's, but few people then believed that a cocktail of pollutants that mimic human hormones could have such profound effects. So nothing was done until it was too late. Over the last few years, an increasing number of...
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...MANUFACTURING BUILDING LOCATED AT 7876 VAN DYKE, MARLETTE, LAPEER COUNTY, MICHIGAN June 2, 2006 May 19, 2006 District Supervisor Remediation and Redevelopment Division Lansing District Office Constitutional Hall North Tower, 4th Floor 525 W. Allegan Street Lansing, Michigan 48909 RE: Category-N Baseline Environmental Assessment of Former Active Homes Manufacturing Building Located at 7876 Van Dyke, Marlette, Lapeer County, Michigan PM Environmental, Inc., Project No. 22-692A Dear District Supervisor: Enclosed is one copy of the above-referenced document prepared in accordance with the March 11, 1999 “Instructions for Preparing and Disclosing Baseline Environmental Assessments by PM Environmental, Inc., on behalf of Outlook Properties LLC. An affidavit signed by Mr. Bob Gilling, for a Disclosure Baseline Environmental Assessment has been included. If you have any questions regarding the information in this report, please contact our office at (248) 336-9988. Sincerely, PM Environmental, Inc. Rachael Koch Project Consultant Steve Price Vice President of Due Diligence Enclosure 22655 S. Chrysler Dr. Hazel Park, MI 48030 Office: 248.336.9988 Fax: 248.336.9989 3340 Ranger Road Lansing, MI 48906 Office: 517.485.3333 Fax: 517.323.7228. 5380 Cascade Suite 200 Grand Rapids, MI 49546 Office: 616.285.8857 Fax: 616.285.8026 PM ENVIRONMENTAL, INC. PROJECT NO. 22-692A...
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...MicroBiology- MLT1 LabPaq / Published by: Hands-On Labs, Inc. sales@labpaq.com / www.LabPaq.com / Toll Free 866.206.0773 A Laboratory Manual of Small-Scale Experiments for the Independent Study of Microbiology 50-0222-MB-01 LabPaq® is a registered trademark of Hands-On Labs, Inc. (HOL). The LabPaq referenced in this manual is produced by Hands-On Labs, Inc. which holds and reserves all copyrights on the intellectual properties associated with the LabPaq’s unique design, assembly, and learning experiences. The laboratory manual included with a LabPaq is intended for the sole use by that LabPaq’s original purchaser and may not be reused without a LabPaq or by others without the specific written consent of HOL. No portion of any LabPaq manual’s materials may be reproduced, transmitted or distributed to others in any manner, nor may be downloaded to any public or privately shared systems or servers without the express written consent of HOL. No changes may be made in any LabPaq materials without the express written consent of HOL. HOL has invested years of research and development into these materials, reserves all rights related to them, and retains the right to impose substantial penalties for any misuse. Published by: Hands-On Labs, Inc. 3880 S. Windermere St. Englewood, CO 80110 Phone: Denver Area: 303-679-6252 Toll-free, Long-distance: 866-206-0773 www.LabPaq.com E-mail: info@LabPaq.com Printed...
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