...the absorption of ultraviolet light by DNA/RNA and its concentration in a sample. The absorption maximum of DNA/RNA is approx 260nm. The purity of a solution of DNA can be determined using a comparison of the optical density values of the solution at various wavelengths. For pure DNA, the observed A260/A280 ratio will be near 1.8. Elevated ratios usually indicate the presence of RNA. The A260/A280 ratio is used to assess RNA purity. An A260/A280 ratio of 1.8-2.1 is indicative of highly purified RNA. The 260/280 ratio below 1.8 often signal the presence of a contaminating protein or phenol. Alternatively, protein or phenol contamination is indicated by 230/260 ratios greater than 0.5. Workflow Time 2 days before the lab session During lab session 1:30 pm Task Cell culture 2:00 pm RNA isolation 5:15 pm Spectrophotometric analysis of your sample Work done by Technician Briefing Student Cell culture (Prepared by technicians) 1 x 105 TM4 cells were seeded in 35mm sterile culture dish with DMEM/10%FBS medium two days before this practical session. Isolation of RNA from cultured cells WARNING: TRIZOL contains phenol and causes...
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...4PY013 MOLECULAR BASIS OF LIFE PROTEIN ASSAY BY BIURET AND FOLIN METHODS Student Number and Name:1316740 Katerina Koupa | Name of Lab Instructor: Dr. J. Walton | Date of the experiment:06/02/2014 | Date of Report Submission:20/02/2014 | (Fig.1) Biuret Reagent Structure ABSTRACT: This report describes the use of the Biuret and Folin methods of protein assay, to verify the protein concentrations of two unknown solutions. To calculate the protein concentrations of the unknowns, nineteen samples were passed through a spectrophotometer which twelve of them had a known protein concentration and there absorbance levels were found. Then a calibration graph was plot that determines the concentration levels of the unknown samples. INTRODUCTION: One of the most typical procedures applied by lab scientist is the protein quantification. Finding protein concentrations in solutions is important in many ways. First of all detecting the levels of a protein in body fluids can result in identifying various diseases. Also protein verification is required for characterization and purification of enzymes. Because of the significance of protein assays laboratories perform this techniques on almost a daily basis. For this experimental procedure, the Biuret and Folin methods were used to determine the protein concentrations of two unknown samples. First the Biuret method (Fig.1) is based on the appearance of peptides bonds in proteins...
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...Perugini Joseph Kim Sarah Hemler Date of submission: May 1st, 2015 Content: Objectives, Hypotheses, Assumptions, Experimental Methods, Data, Data Reduction, Assessment, and Conclusions Pledge: On my honor, as a student at the University of Virginia, I hereby certify that: 1. The work in this report was written collectively by the members of this group and reflects the laboratory and analysis efforts primarily of this group. 2. The data and other findings of the report are entirely those of this group. 3. The group may have discussed experimental problems during the laboratory with members of other groups or with the TA and may have consulted with members of other groups: where such discussions had significant impact on our work, we have noted the impact and the source of the help in the report using a footnote. Pledge signature: Matthew Romano, Anthony Perugini, Joseph Kim, Sarah Hemler (by typing my name here, I am bound to the pledge as if the signature was written by hand.) 1. Objectives Objective 1: Calibrate an elbow flow meter. Objective 2: Determine the useful accuracy and range of commercial flow meters based on vortex generation, magnetic field, and ultrasonic sensing. Objective 3: Determine the “wheel map” for the centrifugal pump: the relationship between flow rate, shaft speed, and head across the pump. Objective 4: Determine the pump efficiency of the centrifugal pump as a function of shaft speed and flow rate...
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...Chromatography INTRODUCTION IN THIS LAB, WE WILL BE EXPRESSING THE GREEN FLUORESCENT PROTEIN (GFP) IN BACTERIA AND PURIFYING IT USING COLUMN CHROMATOGRAPHY. THE SPECIFIC TYPE OF CHROMATOGRAPHY WE WILL BE USING IS HYDROPHOBIC INTERACTION CHROMATOGRAPHY (HIC). THE FOLLOWING IS INFORMATION FROM BIO-RAD INC., THE SUPPLIER OF THE REAGENTS: GFP has several stretches of hydrophobic amino acids, which results in the total protein being very hydrophobic. When the supernatant, rich in GFP, is passed over a HIC column in a highly salty buffer (Binding Buffer), the hydrophobic regions of the GFP stick to the HIC beads. Other proteins which are less hydrophobic (or more hydrophilic) pass right through the column. This single procedure allows the purification of GFP from a complex mixture of bacterial proteins. Loading the GFP supernatant onto the chromatography column When students load the GFP supernatant onto their columns, it is very important that they do not disturb the upper surface of the column bed when performing the chromatography procedure. The column matrix should have a relatively flat upper surface. A slightly uneven column bed will not drastically affect the procedure. However, subsequent steps of loading, washing, and eluting should minimize disrupting the column such that beads "fluff up" into the buffer. When loading the GFP supernatant onto the column, the pipette tip should be inserted into the column and should rest against the side of the column...
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...BIOL 3380 Name:_____________________________________ Circle Session: T-PM W-AM W-PM R-AM R-PM F-AM F-PM Experiment 9 – Pre-lab Homework Enzyme Kinetics of LDH This pre-lab homework assignment is due at the beginning of your lab session. You are provided with the following portion of a protocol: • Determine concentration of enzyme stock solution, if unknown, by taking an A280 nm reading of a 1:100 dilution (in water). Use a total volume of 1 ml in the cuvette. • Dilute some of the enzyme stock with buffer A to make a 4 mg/ml solution. • Serially dilute the 4 mg/ml solution with buffer A to make working solutions of 400 µg/ml and 40 µg/ml. • Prepare 30 µl of each working solution for every sample The PI of the lab gives you a tube of enzyme and tells you the following before disappearing into the office to write more grant proposals: ➢ There is 50 µl of enzyme stock solution. The enzyme is expensive to purify, so follow the protocol exactly, using as little of the stock solution as possible. ➢ The concentration of the stock solution is currently not known, but a 1 mg/ml concentration of the pure enzyme has an A280 nm of 2.0. ➢ You’ll be performing the assay on 12 samples. ➢ Make enough of each working solution so that you have at least 400 ul to work with when you do the assay (to cover any waste and/or inefficiencies in pippetting). Using the spectrophotometer to read the absorbance at 280 nm, you get...
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...haploid, complementation, segregation and independent assortment, meiosis vs. mitosis, genotype vs. phenotype) Develop and test scientific hypotheses Learn to work with a microscope, micropipettor, microcentrifuge and hemacytometer Learn about the genetics of metabolism How this project will be graded: 1. Your scores for Question Sets 1, 2, and 3 will be combined into a single grade. That grade will be included in your Lab Average for the course. Your Lab Average will be included in your final semester average as described in the syllabus for this course. (the deadline for the question submission will be determined in class depending on the progress of the lab) 2. Your scores for Question Sets 4, 5, and 6 will be combined into a single grade. That grade will be included in your Lab Average for the course. (the deadline for the question submission will be determined in class depending on the progress of the lab) 3. You will write a formal lab report in a style and format suitable for submission to the journal Molecular & Cellular Biology. Your report will be included in your final semester...
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...Introduction The profound importance for microorganisms to operate at a maximum efficiency has lead to adaptations allowing for groups of processes to be functional when resources are available, while on the contrary remaining “dormant” when not in need. This has been accomplished at the molecular level by configuring clusters of genes together on the genome into operons that elicit a processive response in the presence of a specific metabolite. The Lac operon is responsible for the cleaving of the disaccharide lactose into two products. A myriad of components control the expression of the Lac operon when two conditions are met. First, the substrate, lactose, must be present. Second, no better substrate for example, glucose, is present (2). The three structural genes in the Lac operon are lacZ, lacY, and lacA. The gene lacZ encodes the tetramer, ß-galactosidase, which is responsible for hydrolyzing the ß-1,4 glycosidic linkage between galactose and glucose in lactose. The transport of lactose into the cell via the enzyme lactose permease is encoded by the gene lacY. The lacA gene encodes the enzyme, galactoside transacetylase, a trimer that transfers an acetyl group from acetyl-CoA to galactosides. Activation of these genes is dependent on the activity of a promoter and three operators based on the nutritional and environmental conditions available to the cell. The lac operon is a negatively controlled inducible operon that utilizes the product of the regulator gene lacI, to...
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...model and the data is collected in the room near it. The data collected issued to reduce the aerodynamic drag and minimize fuel consumption and also increase the speed of the cars, trucks, planes, etc. also aerodynamic properties like lift, drag, forces, moments are measured with the use of wind-tunnel. In wind-tunnel air is blown over the test model which is kept stationary at the throat. This will produce the same effect as it would be produced if the vehicle is moving at high speeds on a road. For more accurate results the tunnel is sometimes equipped with rolling road to prevent the boundary layer forming on the floor which may affect the test results. * What are wind tunnels? Just as its name suggests, a wind tunnel is a tube or tunnel that has man-made windblown through it at a certain speed. Scientists and engineers put a model of an airplane in the tunnel and then study the way air moves around the model. By looking at the way this smaller model acts in the wind tunnel, they get a pretty good idea of how a real life-sized airplane of the same design will probably fly. It is a lot easier, cheaper, and safer to build and test a model than to build and fly a real airplane. * How do wind tunnels work? Wind...
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...PROJECT REPORT FOR AWARDING OF CERTIFICATE ON COMPLETION OF SUMMER INPLANT TRAINING (1st JUNE- 22nd JULY 2011) AT IOCL Gujarat Refinery (Vadodara) SUBMITTED BY: SHREYASH GUPTA Integ.Btech(APE)+MBA(O&G Management) University of Petroleum and Energy Studies,Dehradun PREFACE Though it has been said that best friend a man can ever get is a book but we at this juncture realize that only books cannot give all the information a person seeks. When any student is unable to understand a particular topic, he is advised to imagine the whole matter and then try to understand it. Normally, this method succeeds. But in engineering stream considering the study of wide range of process and equipments involved in it, it is hard to understand the unit operations and processes just through books or even with imagination .Unless one happens to see the process, equipments, he is like a soldier who knows to fire the gun ,but is yet to face a war. Industrial training is one of the most vital part of a syllabus of chemical engineering, which not only teaches one the industrial unit operations, equipments and other technical aspects, but also teaches discipline, interaction with various people irrespective of their posts, the importance of teamwork, etc. This report contains a brief introduction to GUJRAT REFINERY and knowledge gathered about various units in refinery during the training. ACKNOWLEDGEMENT I would like to express...
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...Aacknowledgement First of all I like to thank God almighty who authorise me to research on this topic. I submit my sincere thanks to my supervisor Medhat Khattar for his valuable presence, time, effort, guidance and help to complete this dissertation. My dissertation would not have been completed without the help of lab technicians Nick and Suzy, I am extremely grateful for their help, suggestions and encouragement. I might want to thank my family for impacting in me a comprehension for the significance of education and an appreciation for diligent work. I extraordinarily value the majority of the penances that were made so as to realize the open doors that I have gotten, and it is my trusts that this proposition embodies what I have realized. Much obliged to you for your dedication, bolster, and affection. I might likewise want to thank my grandparents for the numerous hours of math mentoring as a youngster. In spite of the fact that it may have appeared to be inconsequential, it was the premise for my prosperity and the establishment of my hobbies in Designing. I might want to devote this proposition to my family, without whom I would not be seeking after a profession with an instruction from my university undergraduate days. I also thank my supervisor’s effort and good work channelled towards making me a better microbiologist in the world. I sincerely extend my thanks all concerned people who together with me in this regard. Table of Contents I Declaration......
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...0 8 2 C 8 6 8 5 6 2 3 0 0 38 8 8 8 8 6 2 0 0 40 8 8 6 6 6 2 2 2 40 6 6 8 2 Course No EC-1101 CS-1101 MA-1102 ME-1101 PH-1101/ CH-1101 CS-1111 EE-1111 PH-1111/ CH-1111 Course Name Semester-2 Basic Electronics Introduction to Computing Mathematics-II Engineering Mechanics Physics/Chemistry Computing Laboratory Electrical Science Laboratory Physics/Chemistry Laboratory Physical Training –II NCC/NSO/NSS Semester-4 Structural Analysis-I Hydraulics Environmental Engg-I Structural Design-I Managerial Economics Engg. Geology Laboratory Hydraulics Laboratory Physical Training-IV NCC/NSO/NSS Semester-6 Structural Design-II Structural Analysis-III Foundation Engineering Transportation Engineering-II Hydrology &Flood Control Concrete Lab Structural Engineering Lab L 3 3 3 3 3 0 0 0 0 0 15 3 3 3 3 3 0 0 0 0 15 3 3 3 3 3 0 0 T 0 0 1 1 1 0 0 0 0 0 3 1 1 0 1 0 0 0 0 0 3 1 1 1 0 0 0 0 P 0 0 0 0 0 2 2 2 2 2 6 0 0 0 0 0 2 2 2 2 4 0 0 0 0 0 2 2 C 6 6 8 8 8 2 2 2 0 0 42 8 8 6 8 6 2 2 0 0 40 8 8 8 6 6 2 2 MA-1201 CE- 1201 CE -1202 CE -1203 CE-1204 CE-1211 Semester-3 Mathematics-III Building Materials and...
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...2014-2015 Undergraduate Academic Calendar and Course Catalogue Published June 2014 The information contained within this document was accurate at the time of publication indicated above and is subject to change. Please consult your faculty or the Registrar’s office if you require clarification regarding the contents of this document. Note: Program map information located in the faculty sections of this document are relevant to students beginning their studies in 2014-2015, students commencing their UOIT studies during a different academic year should consult their faculty to ensure they are following the correct program map. i Message from President Tim McTiernan I am delighted to welcome you to the University of Ontario Institute of Technology (UOIT), one of Canada’s most modern and dynamic university communities. We are a university that lives by three words: challenge, innovate and connect. You have chosen a university known for how it helps students meet the challenges of the future. We have created a leading-edge, technology-enriched learning environment. We have invested in state-of-the-art research and teaching facilities. We have developed industry-ready programs that align with the university’s visionary research portfolio. UOIT is known for its innovative approaches to learning. In many cases, our undergraduate and graduate students are working alongside their professors on research projects and gaining valuable hands-on learning, which we believe is integral...
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...*3963103* [3963] – 103 T.E. (Petroleum) (Semester – I) Examination, 2011 DRILLING & PRODUCTION OPERATIONS (2003 Course) Time : 3 Hours Max. Marks : 100 Instructions : 1) Question Nos. 1 and 5 are compulsory. Out of the remaining attempt 2 questions from Section I and 2 questions from Section II. 2) Answers to the two Sections should be written in separate books. 3) Neat diagrams must be drawn wherever necessary. 4) Black figures to the right indicate full marks. 5) Use of Logarithmic Tables, Slide Rule, Mollier Charts, Electronic Pocket Calculator and Steam Tables is allowed. 6) Assume suitable data, if necessary. SECTION – I 1. What are different systems on a drilling rig ? Explain any one in detail with suitable diagramme. 18 2. a) Calculate Bottom hole pressure if well depth is 2500 m and mud weight is 1.2 gm/cc. b) Calculate mud weight if mud gradient is 0.87 psi/ft. ′ c) Calculate volume bbl/meter for drill pipe O.D. = 5′ inch and I.D. = 4.276 inch. 2 2 2 10 8 8 16 d) Draw circulation system on a drilling rig. 3. a) Discuss IADC classification of a bit in details. b) Discuss different factors affecting rate of penetration in details. 4. Write short note on : i) Coring ii) Fishing tools iii) BOP iv) Directional well P.T.O. [3963] – 103 -2- *3963103* SECTION – II 5. a) Discuss different types of casings and function of the casings in brief. b) Discuss different types of well completion techniques. 6. a) Discuss primary cementation process with...
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...Dan Brown Deception Point Deception Point by Dan Brown Acknowledgments With warm thanks to Jason Kaufman for his superb guidance and insightful editorial skills; Blythe Brown for her tireless research and creative input; my good friend Jake Elwell at Wieser & Wieser; the National Security Archive; the NASA Public Affairs Office; Stan Planton, who continues to be a source for information on all things; the National Security Agency; glaciologist Martin O. Jeffries; and the superb minds of Brett Trotter, Thomas D. Nadeau, and Jim Barrington. Thanks also to Connie and Dick Brown, the U.S. Intelligence Policy Documentation Project, Suzanne O’Neill, Margie Wachtel, Morey Stettner, Owen King, Alison McKinnell, Mary and Stephen Gorman, Dr. Karl Singer, Dr. Michael I. Latz of Scripps Institute of Oceanography, April at Micron Electronics, Esther Sung, the National Air and Space Museum, Dr. Gene Allmendinger, the incomparable Heide Lange at Sanford J. Greenburger Associates, and John Pike at the Federation of American Scientists. Author’s Note The Delta Force, the National Reconnaissance Office, and the Space Frontier Foundation are real organizations. All technologies described in this novel exist. “If this discovery is confirmed, it will surely be one of the most stunning insights into our universe that science has ever uncovered. Its implications are as far-reaching and awe-inspiring as can be imagined. Even as it promises answers to some of our oldest questions, it poses still...
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...Acknowledgments With warm thanks to Jason Kaufman for his superb guidance and insightful editorial skills; Blythe Brown for her tireless research and creative input; my good friend Jake Elwell at Wieser & Wieser; the National Security Archive; the NASA Public Affairs Office; Stan Planton, who continues to be a source for information on all things; the National Security Agency; glaciologist Martin O. Jeffries; and the superb minds of Brett Trotter, Thomas D. Nadeau, and Jim Barrington. Thanks also to Connie and Dick Brown, the U.S. Intelligence Policy Documentation Project, Suzanne O'Neill, Margie Wachtel, Morey Stettner, Owen King, Alison McKinnell, Mary and Stephen Gorman, Dr. Karl Singer, Dr. Michael I. Latz of Scripps Institute of Oceanography, April at Micron Electronics, Esther Sung, the National Air and Space Museum, Dr. Gene Allmendinger, the incomparable Heide Lange at Sanford J. Greenburger Associates, and John Pike at the Federation of American Scientists. Author's Note The Delta Force, the National Reconnaissance Office, and the Space Frontier Foundation are real organizations. All technologies described in this novel exist. If this discovery is confirmed, it will surely be one of the most stunning insights into our universe that science has ever uncovered. Its implications are as far-reaching and awe-inspiring as can be imagined. Even as it promises answers to some of our oldest questions, it poses still others even more fundamental...
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