...Microbiology Overview Interpretation of preliminary microbiology data Gram-positive cocci Aerobic In clusters ● Coagulase (+): Staphylococcus aureus ● Coagulase (-): Staphylococcus lugdunensis and other coagulasenegative staphylococci In pairs/chains ● Optochin sensitive: Streptococcus pneumoniae ● Alpha-hemolytic: Viridans group Streptococcus, Enterococcus ● Beta-hemolytic: ○ Group A Strep (Streptococcus pyogenes) ○ Group B Strep (Streptococcus agalactiae) ○ Group C, D, G Strep Anaerobic: Peptostreptococcus spp. and many others Gram-positive rods Aerobic ● Large: Bacillus spp ● Cocco-bacillus: Listeria monocytogenes, Lactobacillus spp ● Small, pleomorphic: Corynebacterium spp ● Branching filaments: Nocardia spp, Streptomyces spp Gram-negative cocci Aerobic ● Diplococcus: Neisseria meningitidis, N. gonorrhoeae, Moraxella catarrhalis ● Cocco-bacillus: Haemophilus influenzae, Acinetobacter Anaerobic: Veillonella spp. Gram-negative rods Aerobic Lactose fermenting (Lactose positive): ● Enterobacter spp, Escherichia coli, Klebsiella spp ● Citrobacter spp*, Serratia spp* Non lactose-fermenting (Lactose negative): ● Oxidase (-): Acinetobacter spp, Burkholderia spp, E. coli, Proteus spp, Salmonella spp, Shigella spp, Serratia spp*, Stenotrophomonas maltophilia ● Oxidase (+): P. aeruginosa, Aeromonas spp. Anaerobic ● Large: Clostridium spp Anaerobic: Bacteroides spp, Fusobacterium spp, Prevotella spp. ● Small, pleomorphic: P. acnes, Actinomyces spp *Serratia and Citrobacter spp...
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...melon and the ascorbic acid of a loganberry (http://www.specialtyproduce.com). The sprouting process encourages the rapid growth of microorganisms that reach very high numbers in the finished product (Fu et al., 2000). Seed sprouting provides an excellent environment for the growth of many types of organisms. The release of nutrients from the sprouting seeds, the moisture resulting from the irrigation process, the aerobic conditions, pH and temperatures favorable to mesophiles all contribute to the rapid expansion of microbial population, including food pathogens, during sprouting. Population as high as 109 CFU/g have been reported in sprouts obtained from retail stores (Patterson et al, 1980, Prokopowich et al, 1991). Many published studies have shown that pathogenic bacteria not only survive but can also grow on sprouts under favorable conditions (Portnoy et al., 1976, Harris et al., 1986, Steinbruegge et al., 1988, Abdoul-Raouf et al., 1993, and Farber et al., 1998). The seeds are generally...
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...Pineapple Peelings as Vinegar II. TABLE OF CONTENTS Title Page Abstract…………………………………………………………... 1 Research Plan…………………………………………………….. 2 * Introduction………………………………………………….. 2 * Statement of the Problem…………………………………….. 2 * Significance of the Study…………………………………….. 2 * Scope,Delimitation and Limitations………………………….. 2 * Definition of Terms…………………………………………… 3 Review of Related Literature……………………………………… 4 Methodology……………………………………………………… 5 * Materials………………………………………………………. 5 * Procedures…………………………………………………….. 5 Result of Discussion………………………………………………. 6 * Tabular Presentation………………………………………….. 6 * Analysis………………………………………………………. 6 * Interpretation…………………………………………………. 6 Conclusion………………………………………………………... 7 Recommendation…………………………………………………. 7 References………………………………………………………… 7 Acknowledgement………………………………………………… 8 III. ABSTRACT The pineapple is a tropical plant and fruit. It is an example of a multiple fruit: multiple, spirally-arranged flowers along the axis, each produce a fleshy fruit that becomes pressed against the fruits of adjacent flowers, forming what appears to be a single fleshy fruit. Pimeapples are the only eration fruit in widespread cultivation. It is one of the most commercially important plants which carry out Crassulacean acid metabolism or CAM photosynthesis. The pineapple has a peelings which we considered as waste but in just a simple and easiest way we can make it into another...
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...care professionals state that douching is dangerous, as it interferes with both the vagina's normal self-cleaning and with the natural bacterial culture of the vagina, and it might spread or introduce infections. For example, the U.S. Department of Health and Human Services strongly discourages douching, warning that it can lead to irritation, bacterial vaginosis, and pelvic inflammatory disease (PID). Frequent douching with water may result in an imbalance of the pH of the vagina, and thus may put women at risk for possible vaginal infections, especially yeast infections.[1] In May 2003, a randomized, controlled, multi-center study was conducted with 1827 women ages 18–44 who were regular users of a douche product and who had been treated recently for a sexually transmitted bacterial infection or bacterial vaginosis. Women were randomly assigned to use either a newly designed and marketed douche product or a soft cloth towelette. There was little or no indication of a greater risk of PID among women assigned to use the douche product (versus soft cloth towelette). Douching may be related to a lower probability that a woman becomes pregnant.[2] Antiseptics may also result in an imbalance of the natural bacteria in the vagina, also resulting in an increased likelihood of infection.[3] Furthermore, unclean douching equipment...
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...People with advanced HIV infection are vulnerable to infections and malignancies that are called 'opportunistic infections' because they take advantage of the opportunity offered by a weakened immune system. A partial list of the world's most common HIV-related opportunistic infections and diseases includes: Bacterial diseases such as tuberculosis, MAC, bacterial pneumonia and septicaemia (blood poisoning) Protozoal diseases such as toxoplasmosis, microsporidiosis, cryptosporidiosis, isopsoriasis and leishmaniasis Fungal diseases such as PCP, candidiasis, cryptococcosis and penicilliosis Viral diseases such as those caused by cytomegalovirus, herpes simplex and herpes zoster virus HIV-associated malignancies such as Kaposi's sarcoma, lymphoma and squamous cell carcinoma. Different conditions typically occur at different stages of HIV infection. In early HIV disease people can develop tuberculosis, malaria, bacterial pneumonia, herpes zoster, staphylococcal skin infections and septicaemia. These are diseases that people with normal immune systems can also get, but with HIV they occur at a much higher rate. It also takes longer for a person with HIV to recover than it takes for someone with a healthy immune system. When the immune system is very weak due to advanced HIV disease or AIDS, opportunistic infections such as PCP, toxoplasmosis and cryptococcosis develop. Some infections can spread to a number of different organs, which is known as 'disseminated' or 'systemic'...
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...NINTH EDITION Burton’s MICROBIOLOGY FOR THE HEALTH SCIENCES Paul G. Engelkirk, PhD, MT(ASCP), SM(AAM) Biomedical Educational Services (Biomed Ed) Belton, Texas Adjunct Faculty, Biology Department Temple College, Temple, TX Janet Duben-Engelkirk, EdD, MT(ASCP) Biomedical Educational Services (Biomed Ed) Belton, Texas Adjunct Faculty, Biotechnology Department Temple College, Temple, TX Acquisitions Editor: David B. Troy Product Manager: John Larkin Managing Editor: Laura S. Horowitz, Hearthside Publishing Services Marketing Manager: Allison Powell Designer: Steve Druding Compositor: Maryland Composition/Absolute Service Inc. Ninth Edition Copyright © 2011 Lippincott Williams & Wilkins, a Wolters Kluwer business © 2007 Lippincott Williams & Wilkins, © 2004 Lippincott Williams & Wilkins, © 2000 Lippincott Williams & Wilkins, © 1996 Lippincott-Raven, © 1992, 1988, 1983, 1979 JB Lippincott Co. 351 West Camden Street Baltimore, MD 21201 Printed in the People’s Republic of China All rights reserved. This book is protected by copyright. No part of this book may be reproduced or transmitted in any form or by any means, including as photocopies or scanned-in or other electronic copies, or utilized by any information storage and retrieval system without written permission from the copyright owner, except for brief quotations embodied in critical articles and reviews. Materials appearing in this book prepared by individuals as part of their official duties as U.S. government employees...
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...might be the main cause they aren't the only way in which disease can spread. Prions are one such challenge to this simple germ-disease paradigm. In this paper, the following questions about prion diseases (focusing primarily on kuru and Creutzfeldt-Jakob disease) will be examined: “What are they and what does their discovery mean for our understanding of disease and evolution?” The prion protein (PrPC) is a protein seemingly found in the brains of almost all vertebrate animals which leads to the conclusion that it serves an evolutionarily important role. Due to its affinity for copper and location in the brain, the role PrPC plays in the brain is possibly related to the absorption of excess copper released after a neuron fires. However, studies with mice genetically engineered to lack the PrPC protein suggest that it is not necessary for normal development and that there...
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...each trap will last about two weeks, and then, it can be easily replaced. For increased effectiveness, place multiple traps around your house or property. Procedure Used Get a large 2 liter Coke/Pepsi/Fanta, etc plastic soda bottle. This is the kind that takes a couple of hands to hold onto. Empty the bottle by drinking or draining. Take the cap off and throw away. Just below the point where the neck of the bottle straightens out onto the body of the bottle, cut it across. So now you have a funnel and a cylinder. Invert the funnel into the cylinder. It should fit snugly. A piece of tape will seal it, but may not be necessary. Fill about 1/3 of the cylinder part with a mixture of dry yeast, warm water, and honey (or sugar). Use about 1 Tablespoon of yeast (This is about 1 package of yeast bought at the local grocery and often used to make...
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..."NATURAL PRESERVATIVES" Anthony C. Dweck Research Director, Peter Black Medicare Ltd., White Horse Business Park, Aintree Avenue, Trowbridge, Wiltshire, UK. BA14 0XB SUMMARY This paper looks at the theoretical development of a natural preservative system using the author's data base on medicinal plants as a source of references. The legal aspects of this concept are considered. The traditional methods of preservation, many taken from the food industry are summarised. The use of alcohol, glycerine, sugar, salt, dessication, anhydrous systems and temperature are amongst examples considered. The definitions of the many words used to describe the act of preservation are considered, and the confusion that results from the presence of the many synonyms is considered. e.g. antimicrobial, antibiotic, antiseptic, bactericidal, etc. Specific organisms are identified as being of particular interest, especially those standard organisms that form part of the B.P. challenge test. These include Candida albicans, Pseudomonas aeruginosa, Escherichia coli, Aspergillus niger and Staphylococcus aureus. A cross-section of plants mentioned in the literature as being specifically targeted at these organisms are considered. The paper concludes with Appendices of plant materials that have mention in the literature according to specific definitions, which may give researchers a potential introduction to future research. KEY WORDS Natural preservation, traditional preservation, challenge test organisms...
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...Microbiology? Microbiology is the study of organisms too small to be seen with the naked eye. Originally, emphasis was placed on harmful micro organisms which cause disease or spoilage of beverages and food, but it is now recognized that many microbes have essential roles in our ecosystem or can be used to accomplish beneficial tasks. Human history is full of examples of major devastations caused by bacteria and viruses. Some of these historically important diseases still occur, such tuberculosis and yellow fever. Microorganisms are evolving to cause new infectious disease problems such as Lyme disease and AIDS, which capture public attention. Control and eradication of infectious diseases remain important goals of many microbiologists. The recognition that microorganisms were responsible for what was earlier thought to be "spontaneous generation" opened the door to industrial (pharmaceutical, chemical, energy) and food microbiology, technologies which contribute substantially to today's way of life. Microorganisms in soil and water are essential in the transformation of carbon, nitrogen, oxygen, sulfur and iron to products needed by plants and animals. In various ways, microbes participate in environmental cycling and degradation and global change. In the last few decades, microorganisms have been recognized as ideal model systems for the study of basic biological processes. More recently, microbiologists have brought an exciting dimension to the study of biology through the use of...
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...treated in time, can quickly lead to organ failure and death. Approximately one third of people diagnosed with sepsis die from it. The role of emergency departments throughout the country has become vitally important to the early detection and treatment of sepsis. With the implementation of sepsis protocols in the emergency department the mortality of patients with sepsis admitted through the ED is significantly lower. The word sepsis is commonly used to diagnose patients, but the debate continues as to the true definition. The word sepsis comes from the Greek meaning decay or to putrefy.” (A). Sepsis is a general term that is applied to patients that develop clinical signs of infection. Unlike other diseases sepsis is not diagnosed by the location or type of microbe involved in the infection. Some of the criteria used to diagnose sepsis are abnormalities of body temperature, pulse, respirations, and white blood cell counts. Some symptoms that are common in septic patients are fever, hypothermia, heart rate greater than 90 beats per minute, altered mental status, swelling of the extremities, and high blood glucose in diabetic patients. Sepsis is considered severe when there is organ dysfunction involved. Some examples of this kind of dysfunction are low oxygen level, low urine output; high levels of creatnine in the blood, absent bowel sounds, and low...
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...Case Study One Common Cause Rebecca is having a vaginal discharge for 3 days without other symptoms and significant clinical findings. According to previous study, approximately 75% women have vulvovaginal candidiasis at least once in their live, while 40-50% of them develop 2 or more than two episodes (1). Another common cause of abnormal vaginal discharge, bacterial vaginosis (BV), also have a considerably high prevalence (1). Since Rebecca was having unprotected vaginal sex with her male partner for 9-months, an un-safe sex preference must be considered in this situation. Which means, Rebecca is likely to have unprotected sex with her casual male partners (e.g. no condoms), and STIs must be considered. Trichomonas vaginalis is a common bacterial cause of abnormal vaginal discharge (1), also the commonest non-viral STI across the world (2), it should be considered in this case. Thus, three most likely conditions of Rebecca may be, * Bacterial vaginosis (BV) * Vulvovaginal candidiasis * Trichomoniasis Testing According to the Australian STI Management Guidelines and the STI Treatment Guideline (2010) provided by US CDC, the tests of each listed possible causes can be performed as, 1. BV a) Point of care tests Generally, BV can be diagnosed using gram stain microscopy and Amstel’s criteria (3,4,5). Direct observation of vaginal discharge (using speculum), vaginal pH test (using pH paper) and whiff test (using 10% KOH) need to be performed (3,4...
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...|3.1 Diversity of |Objectives | |Organisms | | |3.1.4 Fungi |State the structure & life cycle of Rhizopus. | | |Explain nutrition in fungi. | | |Outline the structure & reproduction of Yeast. | | |Name 2 Beneficial & 2 Harmful fungi. | | |Mention that there are Edible and Poisonous fungi. | | |Identify and state functions for the following structures: rhizoid, sporangium, gametangium, zygospore. | |3.1.2 + 3.1.5 Handling |1.Discuss and outline the Laboratory Procedures for Micro-organisms | |Micro-organisms |2.State precautions used when working with microorganisms. ...
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...conducted from 1980-2000; this 30 year period saw the report of nearly half of the marine fungal species currently known (Jones et al. 2009; Jones, 2011). That being said, marine fungi are vastly understudied and under rated in comparison to marine plants, animals, and other microorganisms; frequently they are omitted or only briefly referenced in marine biodiversity and ecology text books (Jones and Pang, 2012). The cladistics of marine fungi is currently in a state of flux, with new taxa being discovered as molecular techniques such as DNA and RNA analysis via polymerase chain reactions, and gel electrophoresis are implemented (Ald et al 2005). Even though fungus-like organisms such as oomycetes are not fungi, marine mycologists often study them as they perform similar functions, and until recently most had been classified as fungi based on their morphological similarities (Jones, 2011). These fungus-like organisms are eukaryotic, heterotrophic, zoospores, have chitin containing cell walls, and similar life cycles to fungi (Neuhauser et al. 2012). Conventionally terrestrial or freshwater species are also included in the marine fungal group as facultative species; this is due to their active ecological role in the marine, and estuarine environment. Here is broad, but accepted definition for obligate, and facultative marine fungi from Kohlmeyer, 1979 "obligate marine fungi are those that grow and sporulate exclusively in a marine or estuarine (brackish water) habitat; facultative...
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... Streptococcus, Bifidobacterium, Enterococcus and Lactobacillus. The specific types of Staphylococcus found are: aureus, capitis, epidermidis, hominis. The specific types of Streptococcus found are: mitis, oris, parasanguis, salivarius. The specific types of Bifidobacterium are: adolescentis, bifidum, breve, breve, and longum. Next, the specific types of Enterococcus are: faecalis and faecium, Lastly, the specific types of Lactobacillus are: plantarum and fermentum. (Leónides) According to Dr. Fernandez Leónides and his collegues, studies have shown that certain bacteria in breast milk benefits infants. For example, Lactobacillus, Staphylococcus, Enterococcus, and Bifidobacterium, can sometimes benefit newborns through breastfeeding. The exposure to these bacteria can protect against diarrhea, respiratory diseases, and even reduce the risk of developing other diseases such as diabetes or obesity. However, in many individual cases however, Dr. Leónides points out that certain bacteria are highly personalized with particular individuals in their microbiome. 2) What is the source of these bacteria, where do they come from? The origin of the bacteria present in breast milk was traditionally believed to be from the breast milk that was the...
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