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Ap Biology Lab Report

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Preparatory Procedure
Step 1: Gather all the necessary materials. Place the beaker of enzyme (potato juice) in the plastic container, and fill the surrounding area around the beaker in the container with ice.
Step 2: Take the Sharpie marker and label each of the 7 test tubes as follows. C1, C2(These will be the control variables) 1,2,3,4,5(these will be the experimental variables).
Step3: Once all the test tubes are labeled with the correct number place each one in the test tube rack in in order from C1 to C2 on one side of the rack and test tubes 1 through 5 on the other side of the rack.
Step 4: Using the graduated cylinder, measure out 10mL of the water with a pH of 7. Pour the water into one of the test tubes. Repeat this step until all 7 of the test …show more content…
Starting with test tube 2 place a drop of lemon juice inside (place test tube 1 off to the side for now). Measure the pH just like in step 4 of the Control Variable Procedure. Try to achieve a pH level of 5 by adding more drops of lemon juice if needed. Test the pH after each drop is added.
Step 2: Repeat step 1 for each test tube 3-5. The pH for each test tube should reduce in number and become more acidic. Test tube 3 should be a pH of 4, Test tube 4 should be a pH of 3, and Test tube 5 should be a pH of 2. This all can be achieved by increasing the amount of lemon juice in each test tube.
Step 3: Once all the test tubes 2- 5 have the required pH level, add 10 drops of 0.66% catechol substrate to each test tube 1 through 5.
Step 4: Once each tube is filled with the required amount of substrate, it is time to add the enzyme. Have one lab partner add 10 drops of catechol oxidase enzyme (potato juice) to each of the test tubes. As they add the enzyme, another partner must follow right behind and invert each tube just like before in step 2 of the Control Variable Procedure. Once the first tube is inverted start a timer for 6 minutes like

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