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Bacterial and Blood

In: Science

Submitted By jchros
Words 1671
Pages 7
Observing Bacteria and Blood
Student Jamie Chrostowski
Lab 1 – Due 2/15/15
Microbiology DL1, Professor Newton

Abstract: The different objectives of a microscope allow for a range of observations. At the highest objectives, the resolution can easily be lost which is why the oil immersion lens is used to minimize refraction. While observing specimens, both dead and active, their shapes and arrangements can be observed. This allowed for hands on learning, observation, and identification of the bacterial shapes and arrangements. At the 10x magnification, generally the slides showed tiny dots or it was difficult to properly make any identification. At 100x magnification, identification of structures was possible, such as the nucleus.

Purpose: The purpose of this experiment is to gain knowledge of the functions and operations of the compound light microscope and an immersion oil lens by observing prepared slides of various bacteria slides. Learnings also included the identification and observation of various shapes and characteristics of bacteria, as well as, yogurt cultures (fresh and prepared) under a microscopic view.

Procedure:

Exercise 1: Viewing Prepared Slides * The first step is to assemble the compound light microscope. * Next the slides and cover slips were cleaned for dust and other particles. * Once assembled, the prepared letter “e” slide was placed on the stage and adjustments were made to the condenser, diaphragm, and course/fine adjustment knobs until the image came into clear focus under 10x resolution and again under 40x resolution. * Next, affix the immersion oil lens to the nosepiece. Place a drop of immersion oil onto the slide and slowly rotate the immersion oil lens into place. Use only the fine adjustment knob with the immersion oil lens to bring the object into focus. * Repeat these steps with six prepared slides of…...

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