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Beetroot Ia

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Submitted By JisooKim
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Beetroot IA

Aim * To investigate the structure of a cell membrane
Introduction
The cell membrane is made up of the phospholipid bilayer which is infused with lipids and proteins which keep the membranes structure intact. Acids have properties which allow them to react with the lipids/ proteins- specifically the polypeptide chain; denaturing them which causes the membrane to break. This cause the organelles (such as pigment) in the cells in the membrane are released.

Research question * How does the concentration of HCl affect the structure of the cell membrane of beetroot, measured by light transmittance through the HCl/beetroot solution?

Hypothesis * As the concentration of HCl increases, so too will the density of light transmittance. This is because the more acidic the HCl pH (concentration) becomes the more damaged the cell membrane will become ultimately releasing more and more pigment.

Variables

Independent variable * Concentration of HCl * Range= 0.0%, 12.5%, 25.0%, 50.0%, 100.0% * 0.0%= 100% of distilled Water * 100%= 100% of 2.0 molˉ¹ HCI * Error= ±0.1%

Dependent Variable * The transmittance of light through solution of beetroot pigment without beetroot in percentages. * Error= ±0.01%

Controled/Fixed Variables * a table of the variables that will need to be controlled so as not to influence the results ( make it a fair test).

Variable To Be Controlled | How it will be controlled | Concentration of HCl in solution | Use a 10mL measuring cylinder to measure out 5mL HCl every time | Concentration of distilled water in solution | Use a 20mL measuring cylinder to measure out appropriate amount of every time | Fingerprints or any contact markings of the curvet which effect light transmittance so light travels less through the curvet | Only touch the curvets at the ridged sides

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