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Bio Draft

In: Science

Submitted By Speranpj
Words 841
Pages 4
Effect of Leaf Stomata Density on Variation of Different Environmental Conditions
Author: Patrick Sperandeo
Institution: The Department of Biology, James Madison University
ABSTRACT
Plant structures that are usually found under trees leaves that control water loss are called stomata. Plants control water loss through the opening and closing of the stomata or by varying density. The purpose of this experiment was to see how sunlight exposure affected stomatal density. Since high light density associates with high stomata density, it was predicted that there was more stoma on higher leaves than lower leaves. However, there may be more stoma on lower leaves due to water stress; higher leaves would create shade for lower leaves. Therefore, the effect of differing leaf heights on stomata density was tested. In order to carry out the experiment, samples of 12 leaves were attained from each sample, “sun” and “shade”. Clear nail polish was applied to the samples making an inscription of the stomata. Clear tape removed the nail polish; the tape with the imprint was put on the slide. Under the 400x power, stomata were counted using the slide and the density was determined using a micrometer slide. Afterward a two sample t-test showed significant results at alpha value= 0.05. This supported the alternative hypothesis that varying leaf heights have an impact on stomata density. The prediction that higher levels of leaves topped lower ones when determining stomata was not supported. The opposite was found. A possible explanation was that higher leaves create shade for lower ones so photosynthesis occurs at lower leaves where water loss isn’t high.
INTRODUCTION
Leaf stomata are the basic means of gas exchange in vascular plants. The openings allow for gas exchange and water flow throughout the plants. Gas exchange occurs when CO2 enter the leaves for photosynthesis and

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