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Biology Lab 4 Umuc

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UMUC Biology 102/103
Lab 4: Enzymes
INSTRUCTIONS:

* On your own and without assistance, complete this Lab 4 Answer Sheet electronically and submit it via the Assignments Folder by the date listed in the Course Schedule (under Syllabus). * To conduct your laboratory exercises, use the Laboratory Manual located under Course Content. Read the introduction and the directions for each exercise/experiment carefully before completing the exercises/experiments and answering the questions. * Save your Lab 4 Answer Sheet in the following format: LastName_Lab4 (e.g., Smith_Lab4). * You should submit your document as a Word (.doc or .docx) or Rich Text Format (.rtf) file for best compatibility.

Pre-Lab Questions 1. How could you test to see if an enzyme was completely saturated during an experiment?
- Add more substrate and record the rate. If the rate of the reaction is constant, all the enzymes are saturated.

2. List three conditions that would alter the activity of an enzyme. Be specific with your explanation. * Temperature – Cold temperature will cause the enzyme to work slow, hot temperature will cause the enzyme to increase the movement making it less stable. * PH – Difference in range in the PH scale can alter the shape of the enzyme’s active site * Concentration Of Substrate – Less or more of enzymes to substrates ratio will affect the rate of collisions between the two affecting the number of reactions.

3. Take a look around your house and identify household products that work by means of an enzyme. Name the products, and indicate how you know they work with an enzyme. * Meat Tenderizer – Breaks down larger proteins into smaller proteins to make the meat easier to chew. * Laundry Detergent – When mixed with water they break down stains resulting in clean clothes.

Experiment 1:

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...TYPE YOUR FULL NAME: Ericka Burton TYPE YOUR FULL NAME: Ericka Burton UMUC Biology 102/103 Lab 4: Enzymes Experiment 1: Effect of Enzyme Concentration Table 1: Effect of Enzyme Concentration on the Production of Gas Tube | Amount of yeast | Balloon circumference (cm) After 1 minute | Balloon circumference (cm) After 3 minutes | Balloon circumference (cm) After 5 minutes | Final Circumference (cm) | Time Required to Complete | 1 | 0.05 g | 15.5 cm | 17.2 cm | 20.5 cm | 21.9 cm | 11 mins | 2 | 0.005 g | 13.8 cm | 15.1 cm | 19.6 cm | 20.1 cm | 12 mins | 3 | 0.0005 g | 8.8 cm | 10.5 cm | 13.1 cm | 13.1 cm | 9 mins | Questions 1. What is the enzyme in this experiment? What is the substrate? Yeast is the enzyme. Sugar as the substrate. 2. Did you notice a difference in the rate of reaction in the tubes with different concentrations of enzymes? Why or why not? It is yes, since there is change in balloon size. More the concentration of the enzyme more will be the consumption of substrate. That indicates the rate of reaction is more. 3. What was the effect of using less enzyme on your experiment? There is less size of the balloon for less enzyme concentration. There is less production in the gas. 4. Do you expect more enzyme activity if the substrate concentration is increased or decreased? Draw a graph to illustrate this relationship. If you increase the substrate concentration the rate will also increase up to a...

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