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Biology Urine Analysis

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Submitted By trexrox
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Biology
Lab report- urine analysis

Method: 1. Obtain 10cm3 of one of the urine samples. 2. Examine the urine visually for odour and clarity, describe what you see and record this on results table. 3. Comment on the odour of the urine sample by wafting capours toward your nose with your hand.
Testing for ph 1. Tear off 3cm of universal indicator paper 2. Briefly dip the paper in the urine. 3. Remove the paper and compare its colour against a universal indicator chart. 4. Record ph on results table.
Testing for protein 1. Put 2cm3 of the urine sample into a clean test tube. 2. Put 1cm3 of Biuret A (sodium hydroxide) into test tube. 3. Put 1cm3 of Biuret B (copper II sulphate) into the test tube. 4. Observe colour change and comment and record results.
Test for glucose 1. Put 2cm3 of urine sample into a clean boiling tube. 2. Add 2cm3 of Bennedict’s reagent to the urine. 3. Heat the urine sample in a boiling water bath. 4. Observe any colour change and comment on whether glucose is present.

Results: Samples | colour | Smell | ph | Protein | glucose | 1 | Light straw | aromatic | 9 | absent | present | 2 | Clear | aromatic | 10 | present | absent | 3 | Clear | Clear | 6 | present | present |

Diagnosis:
Ph
Sample 1’s color light straw, indicating concentrated urine. High ADH acting on plasma membrane of collecting duct may be due to insufficient intake of water. Body may be dehydrated. Ph too high, indicating low acidity which may be due to urine tract infection, kidney failure. The same goes for sample 2. Sample 3 has normal ph.
Smell
Sample 3 very likely to be a newborn infant due to clear smell, instead of aromatic smell.
Adults and children usually have aromatic smelling urine.
Glucose
Sample 1 and 3 may be on high glucose diet because generally, no glucose should be present in urine in healthy normal populations. It is either high glucose diet, or reabsorbing of glucose is not effective may be due to urine tract infection, high glucose level may be due to diabetes and insufficient insulin level.
Protein
If sample 2 is an adult, which is highly probable, then the proteins present should be normal depending on the value which is in this case is not measured.
Normal reference values do not contain protein reference value for child and newborn.

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