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Brucella

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Submitted By sakawakaa
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 Zoonotic pathogens : - B.Abortus / B.Melitensis
 Symptoms: -long term fever
- Undulant fever
- Complication in bone manifestation: bone pain ; arthritis
- Re-current infection is common
- Virus like infection
 Identification: - Oxidase + Urease+ -Serology test IgG/ IgM
-gram negative rods,Very small cannot be seen under microscope -Slow grow better isolate with CO2 and increase humidity. -Sampled from blood (5 to 7 days b4 giving a negative report) -cultured on chocolate agar(enrichment media). - Wright test (like widal “ anamnastic reaction” )
 Transmitted via contaminated food Milk products not from human to human : can be killed at high temperature: - tantalization : 50º
- Pasteurization: 70 º
- Sterilization : 100 º
 Treatment: - Tetracyclin
- Doxycyclin
- Rifampin
- For 6 weeks and more
- Vaccine is available for animals.

Vibrio
 V. cholerae, V. parahaemolyticus, V. vulnificus
 Features: - Gram negative
- Fermenter bacilli
- Facultative anaerobes
- Live in halophilic places
 Identification: - Enrichment medium - alkaline peptone broth Vibrios survive and replicate at high pH
- Other organisms are killed or do not multiply
- Selective/differential culture medium - TCBS agar
- V. cholerae grow as yellow colonies
- Biochemical and serological tests
 Pathogenesis and treatment: Rehydration & supportive therapy
Oral Intravenous (IV):
- Doxycycline or tetracycline (Test resistance may be developing) of secondary value
- Water purification, sanitation & sewage treatment Vaccines
 Symptoms: - diarrhea
- feces-streaked stool changes: Colorless; Odorless; No protein
Speckled with mucus

Haemophilus
 H.influenze (meningitidis); H. ducreyi (chanchroid)
 Features: - Gram-negative and pleomorphic,
- Coccobacilli bacteria belonging to the Pasteurellaceae family.
 Identification :- Chocolate agar: Factor V and Factor X
 Treatment: is antimicrobial therapy broad-spectrum cephalosporins
And H. influenza l vaccine

Mycoplasma
 Mycoplasma: - lack a cell wall. Without a cell wall,
- they are unaffected by many common antibiotics such as penicillin or other beta-lactam antibiotics that target cell wall synthesis, including
- M. pneumoniae, which is an important cause of atypical pneumonia and other respiratory disorders,
- Mycoplasma is the smallest known cell and is about 0.1 μ micron in diameter.
 Laboratory diagnosis: - Sputum (usually scant) or throat washings must be sent to the laboratory in special transport medium. It may take 2 -3 weeks to get a positive identification. Culture is essential for a definitive diagnosis
- Serology
 Treatment and Prevention : Since mycoplasmas lack a cell wall, the penicillins and cephalosporins are ineffective. The antibiotics of choice are tetracycline and erythromycin. Prevention is a problem due to the long duration of the disease. It is problematic to isolate patients to avoid close contact for a long period of time. No vaccines are currently available.
Pseudomonas
 Pseudomonas is a gram-negative /oxidase positive bacilli /that belongs to the family Pseudomonadaceae. More than half of all clinical isolates produce the blue-green pigment pyocyanin. Pseudomonas often has a characteristic sweet odor.
 These pathogens are widespread in nature, inhabiting soil, water, plants, and animals (including humans).
 Pseudomonas aeruginosa has become an important cause of infection, especially in patients with compromised host defense mechanisms. It is the most common pathogen isolated from patients who have been hospitalized longer than 1 week. It is a frequent cause of nosocomial infections such as pneumonia, urinary tract infections (UTIs), and bacteremia. Pseudomonal infections are complicated and can be life threatening.
 Isolation : refer to the pic

Chlamidiya
 Gram negative bacilli divided into 2 species Chlamidia and chlamidophilia. strictly aerobes
 Chlamidia is restricted to the human race..chlamidophila hosts the animals
 The most important specie is C. trachomatis.
 Site of infection: urogenital tract
 Treatment: sulfadiazine and tetracyclines.
 Symptoms: since it’s an STD some forms of arthritis, and neonatal inclusion conjunctivitis and pneumonia.
 Identification: PCR , molecular Ab
CORYNEBACTERIA
 Corynebacterium diphtheria
 C. diphtheriae grows best under strict aerobic conditions It is Gram positive
 Colonization of the upper respiratory tract (pharynx and nose) and less commonly skin with C. diphtheriae can lead to diphtheria.
 C. diphtheriae are identified by growth on Loeffler's medium followed by staining for metachromatic bodies (polyphosphate granules, Babes-Ernst bodies).
 Characteristic black colonies are seen on tellurite agar from precipitation of tellurium on reduction by the bacteria. Production of exotoxin can be determined by in vivo or in vitro tests.
Listeria
• Gram +ve rod, individually or in short chains
• Non-spore forming
• Motile by means of flagella
• Optimum growth temperature 30-37°C
• Can grow slowly in refrigerated foods
 Listeria monocytogenes is a Gram-positive aerobic bacilli bacterium. It is the agent of listeriosis, a serious infection caused by eating food contaminated with the bacteria.
 The disease affects primarily pregnant women, newborns, and adults with weakened immune systems
 Listeria species appear as small, Gram-positive rods, which are sometimes arranged in short chains.
 The traditional method is labour intensive and takes up to 5 days to give a result. There are therefore now available many commercial alternatives to generate a faster result. One of these is the use of chromogenic agars following a simple 24 hour enrichment. These agars give presumptive positive results.

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