...Catechol oxidase is an interesting compound to work with, because of this chemical fruits and vegetables turn brown when dropped or cut. I will know how catechol oxidase performs under different pH levels using a spectrophotometer to record its absorbance. This will give the information to determine if more or less product is being made. After performing the test and understanding the results I will compare the difference between absorbance, and if any problems occurred during the process. Introduction Have you walked into a grocery store and noticed that some fruits and vegetables have brown spots on them, they no longer look fresh? For example, when you buy bananas and cut them at home and walk away and ten minutes later they have started to turn brown on the edges. The name for that reaction is catechol oxidase, which is made into the pigment called benzoquinone. In this experiment I will investigate catechol oxidase. The enzyme is a catalyst which makes some chemical reactions occur faster than if no enzyme was present. I will test how well catechol oxidase will function under different conditions, what happens in different pH and the absorbance of each. Catechol oxidase needs copper in order to active, during the process of oxidation electrons and hydrogens are eliminated from catechol. Like stated above the compound...
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...Effect of pH on Enzyme Activity and Benzoquinone Absorption Enzymes are partially or wholly proteins which help catalyze enzymatic reactions. As hypothesized, catechol oxidase (enzyme) was not able to function in a very acidic pH (pH 2) or a highly basic pH (pH 11). In both cases the catechol oxidase activity was decreased which resulted in minimum production of benzoquinone (product). Even though the original hypothesis was not completely supported, however, as seen in figure 1, the absorbance for the mixture is at its highest when the reaction occurs in the presence of a pH 8 buffer, which is close to the pH 7 originally hypothesized. The catechol oxidase is affected by the different pH concentration, and its most favorable pH value is pH...
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...Enzymes activity plays an important role on living organisms. Its activity can be affected by different factors, including environmental and molecular elements. Enzyme inhibition by some molecules can be competitive or noncompetitive according to the binding mechanism. The aim of this study was to determine the type of inhibition occurred by the phenylthiourea (PTU) on the catechol oxidase enzyme. Different substrate concentrations, 0.5, 1, and 1.5 mL, and presence (1mL) and absent of PTU was analyzed. Products were measured at 380 nm. Results showed product only when PTU was not present, except for in one tube. A PTU noncompetitive inhibition is proposed based on the inability to obtained product even when the substrate concentration is increased....
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