...Lab Report Cells as a Source of Energy Answer the following questions about the results of this activity. Record your answers in the boxes. Send your completed lab report to your instructor. Don’t forget to save your lab report to your computer! Activity 1 Record your data from Activity 1 in the boxes below. Enter the samples you added for each trial (methylene blue, glucose, etc…) in the “Variable Added to the Cell Culture” column and the corresponding electrical output results in the “Millivolts Produced” column. | | | | |Trial |Variable Added to the Cell Culture Chamber |Millivolts Produced | |1 |None | | | | |0 | |2 |Flavin Mononucleotide Without light |5 | |3 |Flavin Mononucleotide With light |50 | |4 |Sodium Bicarbonate With light |65 ...
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...The Diversityof Life Lab Manual Stephen W. Ziser Department of Biology Pinnacle Campus for BIOL 1409 General Biology: The Diversity of Life Lab Activities, Homework & Lab Assignments 2013.8 Biol 1409: Diversity of Life – Lab Manual, Ziser, 2013.8 1 Biol 1409: Diversity of Life Ziser - Lab Manual Table of Contents 1. Overview of Semester Lab Activities Laboratory Activities . . . . . . . . . 2. Introduction to the Lab & Safety Information . . . . . 3 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5 15 30 39 46 54 68 81 104 147 3. Laboratory Exercises Microscopy . . . . . . Taxonomy and Classification . Cells – The Basic Units of Life . Asexual & Sexual Reproduction Development & Life Cycles . . Ecosystems of Texas . . . . The Bacterial Kingdoms . . . The Protists . . . . . . The Fungi . . . . . . . The Plant Kingdom . . . . The Animal Kingdom . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12 13 17 22 26 29 . 32 . 42 . 50 . 59 . 89 4. Lab Reports (to be turned in - deadline dates as announced) Taxonomy...
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...a comparison of the optical density values of the solution at various wavelengths. For pure DNA, the observed A260/A280 ratio will be near 1.8. Elevated ratios usually indicate the presence of RNA. The A260/A280 ratio is used to assess RNA purity. An A260/A280 ratio of 1.8-2.1 is indicative of highly purified RNA. The 260/280 ratio below 1.8 often signal the presence of a contaminating protein or phenol. Alternatively, protein or phenol contamination is indicated by 230/260 ratios greater than 0.5. Workflow Time 2 days before the lab session During lab session 1:30 pm Task Cell culture 2:00 pm RNA isolation 5:15 pm Spectrophotometric analysis of your sample Work done by Technician Briefing Student Cell culture (Prepared by technicians) 1 x 105 TM4 cells were seeded in 35mm sterile culture dish with DMEM/10%FBS medium two days before this practical session. Isolation of RNA from cultured cells WARNING: TRIZOL contains phenol and causes...
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...MicroBiology- MLT1 LabPaq / Published by: Hands-On Labs, Inc. sales@labpaq.com / www.LabPaq.com / Toll Free 866.206.0773 A Laboratory Manual of Small-Scale Experiments for the Independent Study of Microbiology 50-0222-MB-01 LabPaq® is a registered trademark of Hands-On Labs, Inc. (HOL). The LabPaq referenced in this manual is produced by Hands-On Labs, Inc. which holds and reserves all copyrights on the intellectual properties associated with the LabPaq’s unique design, assembly, and learning experiences. The laboratory manual included with a LabPaq is intended for the sole use by that LabPaq’s original purchaser and may not be reused without a LabPaq or by others without the specific written consent of HOL. No portion of any LabPaq manual’s materials may be reproduced, transmitted or distributed to others in any manner, nor may be downloaded to any public or privately shared systems or servers without the express written consent of HOL. No changes may be made in any LabPaq materials without the express written consent of HOL. HOL has invested years of research and development into these materials, reserves all rights related to them, and retains the right to impose substantial penalties for any misuse. Published by: Hands-On Labs, Inc. 3880 S. Windermere St. Englewood, CO 80110 Phone: Denver Area: 303-679-6252 Toll-free, Long-distance: 866-206-0773 www.LabPaq.com E-mail: info@LabPaq.com Printed...
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...Biology 100 – K. Marr (Revised Spring 2010) Lab 2. Microscopic Observation of Cells Prelab Assignment 1. Before coming to lab, read carefully the introduction and procedures of each part of the experiment. If you and your group members are not familiar with the procedure before coming to lab, you may have difficulty completing this exercise during the lab period. 2. Answer the Prelab Questions on the first three pages of the report sheet and be prepared to hand them in at the start of your lab class. Please be aware that you need to go online to answer prelab question #3. Goals of this Lab Exercise After completing this lab exercise you should be able to..... 1. Identify the parts of a compound light microscope and use a microscope to competently examine biological samples 2. Determine the diameter of the field of view for the various objectives of a microscope 3. Accurately sketch, describe and cite the major functions of the structures and organelles of the cells examined in this lab exercise 4. Estimate the size of specimens viewed with a microscope. The Microscope The microscope is one of the principal tools of the biologist. Without the microscope, many of the great discoveries of biology would never have been made. The light compound microscope, illustrated in Figure 1, is the type of microscope most commonly used. Proper, comfortable use of the instrument demands practice. The practice afforded you in this exercise depends upon familiarity with the parts of the microscope...
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...Comparing Growth of E. Coli at 37°C and 25°C and on rich and minimal mediums Lab report 1 Richard Montez MCB 3020L Monday 2 pm June 15, 2013 Abstract: Bacteria is a very diverse creature, and can grow in extreme conditions. There are two variables that factor in finding out what are more optimal conditions in which E. coli can grow, temperature and difference in medium. The two ways to determine the amount of bacteria growth are: optical density by using the spectrophotometer, and also the 10-fold serial dilution, the latter of which is the most accurate way. The 4 phases: lag phase, log phase, stationary phase and death phase, show the strength of the life cycle of a bacteria cell. The results show that at 37°C (normal body temperature) and the LB agar plate (rich medium) gives E. coli better optimal conditions to grow. Introduction Although bacteria is composed of only a single cell, they are extremely involved and we have so much to learn about these creatures. Bacteria can live in some of the most extreme temperatures- from temperatures that could potentially freeze the blood under your skin, to the other end of the spectrum where they could be found at temperatures near 100°C at the mid-ocean volcanic vents. The bacteria we chose to further learn about it's optimal growth conditions was Escherichia Coli. There are two variables in this experiment, temperature and choice of medium. The first variable, the sample was incubated E. Coli at 25°C...
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...Yeast Culture Lab Introduction Yeast is a one-celled, microscopic organism, which is part of the fungi kingdom. Yeasts do not make up a single group (Smith & Smith, 2012). Yeasts use organic material as a means of making energy, which make them chemoorganotrophs (Smith & Smith, 2012). Carbon is procured primarily from hexose sugars, such as fructose and glucose. Yeast need either oxygen for aerobic cellular respiration or for species that are anaerobic, but also have aerobic methods creating energy (Smith & Smith, 2012). There are no species of yeast species that are known to grow only anaerobically. Yeasts thrive in an environment with a slightly acidic (Smith & Smith, 2012). The reproductive cycle of yeasts can be either asexual or sexual depending on the species. The most widely seen method of growth in yeast is asexual reproduction referred to as budding (Smith & Smith, 2012). Reproduction in reference to yeast depends on the species; the species can be both asexual by mitosis and sexual by budding (Smith & Smith, 2012). Consumption refers to use and the rate of use of something such as how a consumer, such as a primary consumer like a tree would use photosynthesis to make energy from carbon dioxide. Death in reference to a population is referring to the rate of death in that population (Smith & Smith, 2012). Hypothesis The primary goal of the yeast culture lab is to test a theory involving samples of yeast cultures grown...
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...MBK – Lab Report Name: __Jade Smart___ Section: ___________________ Aseptic Technique and Culturing Microbes Part 3: Generating Microbial Cultures: Observe your organisms after 24 hours to assess the growth patterns of all tubes. If there is no observable growth allow the tubes to incubate an additional 24 hours. Record your observations. Questions: A. What is the difference between a bactericidal and bacteriostatic agent? Between sterilization and disinfecting? The difference between the two is that bactericidal kills bacteria directly. While bacteriostatic stops the bacteria from growing. Bactericidal will injure the plasma membrane and the cell will leak out, killing it. Bacteriostatic stops bacteria from replicating. The main difference between sterilization and disinfection is, that sterilization kills all microorganisms, while disinfection eliminates harmful microorganisms from inanimate objects and surfaces. Sources: http://study.com/academy/lesson/types-of-antibiotics-bacteriocidal-vsbacteriostatic-narrow-spectrum-vs-broad-spectrum.html http://www.diffen.com/difference/Disinfect_vs_Sterilize B. List five sterilization methods, how they work, and what they are used for. The first form is steam. A machine called an autoclave is heated to 121-134 degrees Celsius. You hold the object there for 15 minutes for 121 degree Celsius or 3 minutes at 134 degree Celsius. It is used to inactivate all fungi, bacteria, viruses,...
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...“CDC had never advised against travel to any region, even during the plague epidemic in Indian in 1994, until the SARS outbreak. 2. The CDC-wide activation for SARS marked the first use of its newly created Emergency Operation Center, built as a result of lessons learned from our 2001 response to the anthrax bioterrorism event, where 1,700 CDC staff responded without a dedicated EOC. 3. Deployment statistics calculated that CDC staff contributed the equivalent of 46,714 days of work devoted to the SARS response. Another 71 people volunteered but were not deployed by the end of the response. 4. Of the eight lab-confirmed cases of SARS in the United States, six were identified in the first month surveillance for SARS began. Five traveled to Hong Kong, two to Toronto, and one to Singapore. 5. Among people with SARS in the United States, the majority were male (53%) with a median age of 39. 6. In the United States, the only possible case of secondary spread was between a married couple and both had traveled internationally. 7. SARS-CoV comes from the family of viruses that also cause the common cold in...
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...of America. * Testing and research on these therapies is continuing in Bio Viva’s affiliated labs worldwide. * We should spend many days thinking, speaking and writing about the future, to find out the what are the new theories are coming out in this world. The one of the famous writer Warsaw was speaking at an Innovation Conference, in London speaking at a Global Leadership Summit, which was being interviewed by the Discovery Channel to find out, whatever the situation, I have one singular mission. I want you to think about the future. * The change in our lives, our economy and business to think about the future now. * One of most outgoing emerging technology is age reversal. Age Reversal It’s an extra ordinarily expensive, complex and risky for the people who want to turn back the clock. Actually according to nature scientific reports, it has already reversed aging in human cell by turning off and tuning on the new technology raising nuclear NAD+ (the compound responsible for communication between the nucleus and mitrocondise in your cells). * Old mice reverse metabolic dysfunction to give a compound called nicolinamaide adenine dinucleotide or NAD a week and found age indicator 2 year’s old mice or that 6 month old. * That a result be turning 60 year old human into a twenty year old. * They was a Another study published in CELL reports that...
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...Using Anti-fungal Agents to Reduce Fungal Contamination for Micropropagation in the Classroom Jason Okazaki Mentor: Roger Shane Gold Department of Biology, Brigham Young University-Hawaii, 55-220 Kulanui St., Laie Hawaii Introduction Micropropagation is a method used for the multiplication of tissue culture in vitro. Fungal contamination is a major problem during explant micropropagation, as fungal growths greatly reduces survival and shoot proliferation. Fungal contamination is especially a problem in undergraduate teaching labs where inexperience and suboptimal culturing conditions tend to amplify the problem. The use of antifungal agents in culture may help alleviate these problems (Brown et al. 1982, Sheilds et al. 1984, Hauptmann et al. 1985, Tynan et al. 1993) . The purpose of this study was to explore the use of antifungal chemicals on Saintpaulia ionantha (African Violet), Daucas carota (Carrot), and Passiflora edulis (Passionfruit) by testing the efficacy of five commonly used antifungal compounds (Miconazole, PPM, Amphotericin B, Benomyl, Nystatin) as gauged by monitoring rates of fungal contamination and explant survival during in vitro micropropagation. Results There was a significant difference between the different antifungals used when comparing explant survival (p=0.011). PPM at 1.5 ml/L showed the best result with 75.0% of P. edulis explants surviving. Conclusions All explants of D. carota were contaminated throughout the experiment. This may have...
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...Someone’s Name Bio 488C Lab A Re: Writing Assignment 2: Enterics Report 2/22/2016 Enterics Report The group received the Unknown sample of Patient #2 as well as a description of the illness. The method to identify this unknown organism was an Enteropluri tube. By using the inoculation needle in the Enteropluri tube to sample the organism and draw the sample through the set of agar compartments, multiple culture media are thereby inoculated. Given that this test is used on Enterobacteriaceae all organisms are assumed to be Gram negative and oxidase negative. However, each culture media provides a biochemical test that is used to differentiate and identify the microorganism such as glucose fermentation with or without gas production, lactose fermentation, urea hydrolysis, etc. Each feature is used to differentiate and identify the microorganism in question. While the Enteropluri tube is a useful device, I do not believe it is the most effective method of identification in a modern clinical setting. Possible drawbacks of this device is failing to get a uniform inoculation of each media – as the inoculation needle is drawn through each successive media less and less of the sample is present, meaning the first compartments inoculated may receive more cells which will result in quicker growth, compaired to the final compartments which may receive far fewer cells and result in slower growth and possible false negatives. There is also a concern that the media in each compartment...
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...three years ago, Rebecca Skloot tells the story of Henrietta Lacks, an African-American woman, who became the source of the first line of immortal cells. Henrietta was born Loretta Pleasant in Roanoke, Virginia in 1920. Henrietta lived a typical life for a poor African American of that time - growing up on her family's tobacco farm until her mother’s death. By 1950, Henrietta had married her first cousin, David “Day” Lacks, birthed five children, and relocated to the Baltimore, Maryland area. In January of 1951, Henrietta went to the “colored” ward of Johns Hopkins Hospital complaining of a “knot” in her lower abdomen. It was found that the knot feeling was due a dangerous and growing tumor in her cervix. After a formal diagnosis of cervical cancer, samples of Henrietta’s cervix were removed unbeknownst to her. The biopsy samples were given to Dr. George Gey, a tissue culture specialist. He was working on creating an immortal cell line to be used for human medical research. He discovered that Henrietta’s cells, later known as “HeLa” cells, were very unique because they grew exponentially faster than standard cell lines and never died. Henrietta Lacks died at the early age of thirty-one due to the her metastasized cancer. Interestingly, her death was just the beginning of her legacy. Knowing the true value and potential of the cells, Dr. Gey provided the...
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...Khan 7 7 7 01:30 -03:00 Sec No. Room No. Teacher's Name 1 Introductory Biochemistry Introduction to Biotechnology Molecular Biotechnology A A A 9 35 4 Sci Y Sci Y Sci Y 3 3 Microbiology Electricity and Magnetism A A 56 31 NB-15 NB-8 Data Analysis & Report Writing A Data Analysis & Report Writing B Data Analysis & Report Writing C 33 NB-14 Farah Arif Munaza Bajwa Itrat Batool Naqvi 21-Oct-13 1 5 41 Main Lab NB-7 1 1 English-I English-I N K 25 44 SCI Y SCI Z Sadia Ghaznavi Nasreen Pashsa 3 Mathematics A 28 NB-36 Nighat Altaf 5 Molecular Physiology A 16 SCI 9 SCI 6 SCI 8 SCI 12 SCI 12 Tooba Mohtsham Asifa Kayani Saleha Mehboob Ayesha Aftab Gaitee Joshua 22-Oct-13 Basic Concepts of Environmental Sciences 24 5 A Data Handling and Atomic Spectroscopy 5 A 5 5 Electrical Instrumentation Human and Animal Behavior A A 9 9 12 7 Advanced Topics in Molecular BiologyA 7 7 Medical Biotechnology Plant Ecology A A 19 33 3 SCI 6 SCI R SCI 6 SCI 7 SCI 7 SCI 8 SCI8 Dr.Hooria Younas Dr. Amber Shehzadi Asifa Kayani Ayesha Roohi Saleha Mehboob Saima Mubeen Dr. Saleema Bashir 3 3 Cell Biology Molecular Biology A A 22 37 SCI 12 NB 15 Amna Younus Dr. Amber Shehzadi 1 Introduction to Inorganic Chemistry A 1 Basic Concept of Organic ChemistryA 1 Calculus I A 21 35 27 NB-9 SCI 12 NB-9 Rahila Tariq Ayesha Roohi Shumaila Waheed 23-Oct-13 Oxidation-Reduction Reactions...
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...Clinical Microbiology Lab Final December 13, 2013 Table of content Gram Stain Technique……………………………………………………………………………………………… page 1 Culture Transfer Technique……………………………………………………………………………………… page 2 Acid-Fast Stain Technique………………………………………………………………………………………… page 3 The importance of the Gram Stain Technique to a physician……………………………………. page 4 The importance of varying shapes/colonies formation of bacteria……………………………. page 5 Spore Stain Technique………………………………………………………………………………………………. page 6 The Importance of incubation/protocol techniques…………………………………………………... page 7 The importance of various types of media for bacterial growth…………………………………. page 7 The importance of biochemical analysis in the microbial process……………………………… page 8 The importance of studying Clinical Microbiology and how the course will assist me in reaching my professional goals……………………….. page 9 Bibliography……………………………………………………………………………………………………………… page 10 Gram Stain Technique The Gram Stain is one of the most important differential stains used in bacteriology. (Cappuccino and Sherman, Microbiology A Laboratory Manual) Using the gram stain it is possible to determine purple gram-positive cells (S. aureus) from pink gram-negative cells (E. coli). The results of the Gram Stain make it possible to identify microorganisms by their shape, number and morphology. In a clinical setting these results can help in treatment by identifying the type of microorganism...
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