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Cheese Lab Stuff

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Cheese Lab
Background: Enzymes are proteins that catalyze or control metabolic reaction. Enzymes work by lowering the amount of activation energy needed so the reaction will happen more quickly. The molecules that an enzyme acts upon are called substrates, the substrate solutions used in this lab were milk and water. In this lab, the enzymes are specific for particular substrates. The enzyme (junket tablet) converts these substrates into different molecules by curdling. If the enzyme concentration required to start the reaction increases and speeds up a reaction, then it will remain unchanged unless certain limiting factors are introduced.
Analysis & Discussions:
Enzyme concentration: According to figure 5, if the amount of concentration of junket tablet increased, the time for initial curdles to form in room temperature milk decreased. This was evident because the curdles that were formed on the edges of the test tube was much faster as the amount of enzyme concentration increased by 1/5th each. The trend shows the inclination of time versus the concentration of the junket tablet, indicating the presence of a higher amount of enzymes controls the decrease in time. Moreover, the curdles were then formed larger by the last few trials according to table 5. The hypothesis is proven true by figure 5, where the rate of reaction increased as the enzyme concentration increased except at trial four where it decreased slightly. In conclusion, the higher the enzyme concentration, the higher the rate of reaction will be because having more enzymes means more reactions can happen at once and since each enzyme decreased the activation energy, the rate of reaction increased. pH: As the pH level of substrate increased, the time taken for the milk to curdle also increased. Therefore as the pH level increases, the time in activity of the enzyme will also increase.

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