...GOLD BUYING PROCEDURE Undocumented/treasure AU Bars PRIOR OR BEFORE IMPLEMENTATION A TABLE MEETING IS A MUST FOR M.O.A SIGNING BY BUYER & SELLER. For sponsored project (Seller is not the one paying the Contingency Fee/ Intermediary/ies paid the fee ) both Parties have agreed that a day before implementation, First Party (SELLER ) will hand over the _____ grams of sliced gold grams from subject gold bars to Second Party ( BUYER ) as “commitment” and to be given to the buyer’s verifier at the venue a day prior ( before ) to implementation as “COMMITMENT” rest in three MAJOR reasons: Assurance gold slice quantity varies as follows : METRO MANILA 100 grams LUZON 200 grams VISAYAS & MINDANAO 300 grams. 1. Avoid last minute back out. ( pag-urong ng seller sa araw ng bilihan / paglabag sa pinirmahang kasunduan ) 2. Avoid switching ( pagpalit ng item na peke ) 3. Serve as key for the Buyer as assurance for expenses incurred ( in caase of seller’s failure ) ( ang sliced grams of gold ay magsilbing pantapal sa nagastos ng BUYER, kung sakali man magkaroon ng hindi pagkatuluyan sa transakayon sa anumang dahilan nilabag ng SELLER sa araw ng buying/implementasyon. ) MIMINUM BUYING PER IMPLEMENTATION 6.2 KG. ( 5 BARS ) 12.5 KG ( 3 BARS ) 74.6 KG. ( 1 BAR ) Transaction/s wherein the Seller or intermeiaries pays for the Contingency Fee for its implementation...
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...culture, is the removal of cells from an animal or a plant, and growing the cells in a favourable artificial condition (Wiley, J.M et al, 2013). There are three types of cell culture: primary, this is cultivating the cells away from the parental tissue, secondary, which is when a primary culture is sub-cultured and cell lines, which can be continuous or finite depending on the life span of the culture. Virus titrations are used to estimate the virus concentration; it is a viral quantification technique. When detecting the virus, the cytopathic effect is looked at, whether there is lysis of the cells, vacuolation, formation of syncytia and the presence of inclusion bodies. TCID50, is the measure of the infectious titre. The end point dilution assay quantifies the amount of virus that is required to kill 50% of infected hosts or to produce a cytopathic effect in 50% of inoculated tissue culture cells (Kumar P, 2013). The purpose of the virus titration within tissue culture is to isolate and identify viruses within clinical samples, to carry out research on the viral structure, replication, genetics of the virus and the effect on the host cells, and also to prepare viruses for vaccine production. Results Table 1 Data used to determine the 50% endpoint using the Reed-Muench method Log of virus dilution | Infected test units | Cumulative infected (A) | Cumulative non-infected (B) | Ratio A/(A+B) | Percentage infected (%) | -1 | 5/5 | 37 | 0 | 38/38 | 100 | -2 | 5/5 | 32 | 0...
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...Introduction Ash is a vital ingredient in the canine diet as it is the main constituent of minerals within most diets. Ash content in food is determined by burning the food to calculate the ash percentage left behind. A deficiency in any of the minerals within ash would result in a wide range of health problems which is why it is a legal requirement to display the ash content on food packaging. This experiment is designed to recreate the process undergone by the pet food company to determine the ash content within the food. Method Weigh silica crucible and record weight prior to experiment. Grind over 5g of food sample in pestle and mortar and measure 5g of ground sample into the crucible. (W0) Place the dish in a baking oven for 24hrs to dry out the sample. Reweigh the sample in silica crucible, This is now the true weight of the sample (W1) Set up the bunsen burner on top of the heatproof mat with tripod and clay pipe triangle above to support the crucible. Ignite the bunsen burner and begin to heat the sample slowly ensuring the sample does not set on fire as this will result in a loss of material to the atmosphere in the form of particles. The sample should turn black. Once the sample reaches roughly 100°C continue to heat to 500°C until the sample turns white. Allow sample to cool to room temperature in a dessicator. Weigh the finished sample (W2) Calculate the ash content (X): (W2-W0)/(W1/W0)x100=X Materials: Silica dish Baking Oven Bunsen Burner ...
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...4110065A.qxp 9/25/2007 2:39 PM Page 1 ™ Quick Start Bradford Protein Assay Instruction Manual For technical service call your local Bio-Rad office, or in the US, 1-800-4BIORAD (1-800-424-6723) 4110065A.qxp 9/25/2007 2:39 PM Page 5 Table of Contents Section 1 Introduction 1 1.1 Principle 1 1.2 Selecting a Protein Standard 5 1.3 Product Description Section 2 Instructions 9 11 2.1 Standard Assay Protocol 11 2.2 Microassay Protocol 14 Section 3 Data Analysis 18 Section 4 FAQs and Troubleshooting 22 Section 5 Ordering Information 26 Section 6 References 28 Section 7 Appendix 30 4110065A.qxp 9/25/2007 2:39 PM Page 7 Section 1 Introduction The Quick Start Bradford protein assay is a simple and accurate procedure for determining the concentration of protein in solution. It provides ready-to-use convenience by supplying the dye reagent at 1x concentration and two protein assay standards at seven prediluted concentrations. The prediluted standards are conveniently packaged in 2 ml screwcap vials, eliminating wasteful and sharp ampoules, and ensuring protein stability over the shelf life of the product. 1.1 Principle The Bradford assay is a protein determination method that involves the binding of Coomassie 1 4110065A.qxp 9/25/2007 2:39 PM Page 8 Brilliant Blue G-250 dye to proteins (Bradford 1976). The dye exists in three...
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...Animal Nutrition Lab Reports FdSc: Animal Health and Welfare STE09288743 Practical 1 - Determination of the ash content of foods Introduction Foodstuffs are divided into five categories; crude protein – the total level of nitrogen in a feed, ether extract – the total fat content of a feed, nitrogen free extract – the soluble carbohydrate in a feed, crude fibre - cellulose, fibre with nitrogen attached & alkali insoluble lignin and total ash content – an indication of the mineral content of feeds. The aim of this report is to try and find out the total ash content of Wainwrights Adult Complete Dog Food With Duck And Rice through a food analysis method and compare the amount found with what the original packaging states. Mclements (2003) explains that “ash is the inorganic residue remaining after the water and organic matter have been removed by heating in the presence of oxidizing agents, which provides a measure of the total amount of minerals within a food.” It’s important for the correct amount of ash to be in a dog’s diet because as Professional Pet Foods (2013) explain, “excess ash will contribute excess minerals to the diet, potentially leading to health problems.” Method This method can be used for all types of food containing less than 50% fat, it allows for the determination of the mineral content of foodstuffs. In this process, the samples temperature is taken to 550°C to ensure all organic matter is...
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...Baghpat Crossing, MEERUT 250 005 UP, India 2 Department of Pharmaceutics, Laureate Institute of Pharmacy, KATHOG 177 101 The. Dehra, Dist. Kangra, HP, India Abstract: Piper betle leaves (Family: Piperaceae) have been used in Chinese and Indian folk medicine for centuries and recently been proposed to be used as a chemopreventive agent because of its anti-oxidant activity. In the present paper, Piper betle leaves were standardized for stomatal index, vein islet and vein termination numbers, palisade ratio, UV fluorescence and different ash values. The Piper betle leaves are earlier reported to possess anticancer potential. Hence, the aqueous extract of the leaves was subjected to cytotoxicity studies on Hep-2 cell line using MTT and SRB assays. The mean CTC50 was found to be 96.25 µg/mL, which proved the potent cytotoxicity and hence, the probable anticancer property of the selected extract. Key words: Betel Leaf Anticancer Phytochemical characterization and its leaves, with a strong pungent and aromatic flavour, are widely consumed as a mouth freshener [4]. The leaves are credited with wound healing, digestive and pancreatic lipase stimulant activities in the traditional medicine [5]. During our exploration of non-toxic and affordable herbal medicinal formulations, the PBL extract and its constituent phenolics were found to show impressive anticancer activities [6-8]. The deep green heart shaped leaves of betel vine are popularly known as Paan in India [9, 10]. MATERIALS AND...
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...have higher peroxidase activity levels because it was under more stress than the plant that was watered with water. Through the use of such techniques as tissue prints, dot-blot assays, electrophoretic analysis, and spectrophotometric assays, peroxidase activity levels were visualized and quantified. The results were as expected; the tissue from the plant that had been treated with Vitamin Water was more purple on the tissue prints and it was spectrophotometrically determined that the plant that had been treated with Vitamin water had a higher amount of peroxidase activity than the one that was treated with water. The results proved that the hypothesis was correct; stress on a plant, Vitamin Water in this case, causes higher levels of peroxidase activity. Introduction: Peroxidases are enzymes that use hydrogen peroxide as an electron acceptor to catalyze oxidative reactions. Peroxidase is believed to play a major role in synthesizing cell walls, preventing toxins and responding to stress. It is responsible for the purple color that is seen throughout the experiments. Peroxidase can be found in different locations in a plant in the form of isoenzymes. Many different experiments can be done to locate, visualize and quantify the activity of peroxidase enzymes. These include, tissue printing, dot-blot assays, electrophoresis, and spectrophotometry. This knowledge of what peroxidase does and how its activity levels can be detected was used to develop...
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...cancer [2]. Chemotherapy is restricted by both intrinsic and acquired cell resistance to drugs. [3] This has necessitated the use of natural products for the treatment of cancer. There are compelling evidences for experimental investigations on the efficacy of plant drugs against cancer [4]. It is known that there are links between the inflammatory and nociceptive, oxidative and cancer processes. The ability to inhibit any of the process will definitely lead to the inhibition of the others [5]. Based on the fact that M. calabura fruit possess antioxidant activity, the aim of the present study was to determine the in vitro anticancer activity of methanol extracts of the fruits of M. calabura against HeLa and HEp-2 cancer cell lines using MTT assay. Plant Material and Extraction Collection and authentication of fruits of M. calabura Linn were collected from the surrounding areas of Erode, Tamil Nadu state, India during 2008-2009 and the plant was identified and authenticated and deposited (voucher number: BSI/SC/5/23/09-10/Tech-132) at Tamil Nadu Agricultural...
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...The Brad assay * is one of the most common forms of measuring absorbance through a spectrometer * uses a a dye (coomassie Brilliant Blue G-250) that binds to proteins and is absorbed at a wavelength of 465- 598 nm * Fast, therefore can meaure a 96 plate in 5 minutes * Absorbance shift allows the measure the protein at the 598 nm wavelength by using the spectrometer, can be justified through the beer-lambert law * The standard curve can be used to estimate the concentration of an unknown protein in solution by * Taking in account their known absorbance levels * Use line equation from curve * The unknown protein conc. in a sample with a known absorbance level can be determined Bradford Vs. Lowry * Lowry * Uses alkaline conditions- reduction cu 2+ to Cu+ * Folin phenol reagent- yellow to blue * Peptide bonds for monocovalent copper and radicals of try and trp react with folin * Concentrations 0.1-1.0 ug/ml of protein * Can be interfered with easily * Slow 40-60 * Colour varies with proteins * Criticial timing with procedure * * Bradford * Based on absorbance shift from brilliant blue when bound to proteins * 465- 598 (when bound) * Acidic conditions * Binds tightly to protein, and inhibits binding sites (little inference) * Limitations * Strong basic buffers Detergents interfere with binding of the cosmic blue, causing...
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...4PY013 MOLECULAR BASIS OF LIFE PROTEIN ASSAY BY BIURET AND FOLIN METHODS Student Number and Name:1316740 Katerina Koupa | Name of Lab Instructor: Dr. J. Walton | Date of the experiment:06/02/2014 | Date of Report Submission:20/02/2014 | (Fig.1) Biuret Reagent Structure ABSTRACT: This report describes the use of the Biuret and Folin methods of protein assay, to verify the protein concentrations of two unknown solutions. To calculate the protein concentrations of the unknowns, nineteen samples were passed through a spectrophotometer which twelve of them had a known protein concentration and there absorbance levels were found. Then a calibration graph was plot that determines the concentration levels of the unknown samples. INTRODUCTION: One of the most typical procedures applied by lab scientist is the protein quantification. Finding protein concentrations in solutions is important in many ways. First of all detecting the levels of a protein in body fluids can result in identifying various diseases. Also protein verification is required for characterization and purification of enzymes. Because of the significance of protein assays laboratories perform this techniques on almost a daily basis. For this experimental procedure, the Biuret and Folin methods were used to determine the protein concentrations of two unknown samples. First the Biuret method (Fig.1) is based on the appearance of peptides bonds in proteins...
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...Changing My Religion Converting from Christianity to Islam Crystal K. Baldwin Religion and Philosophy: Humanities 400 Professor Phillip Van Vleck Abstract I Love The Lord…He Heard My Cry I’ve always had a strong faith in our Heavenly Father. I can do all things through Christ who strengthens me. My views and beliefs have developed over a long period of time and have changed as I have grown in my faith and knowledge. In the past few years my family and I have gone through many trials and tribulations. Test on my faith has caused my spiritual confidence to falter, so I turned to reading, researching and asking questions. I’ve always had an interest in Islam. My eldest son recently converted from Christianity to Islam. I asked him why he converted and his answer was “because he always questioned the Jesus thing”. He said he never understood how Jesus died for our sins and we continue to sin. I found his statement very profound; it caused my own doubts and me to think about my many years of being a Christian. Muslims believe that Christians are committing shirk by referring to Jesus as the Son of God and believing in the Trinity (which, according to the Qu’ran, consists of God, Mary and Jesus – 5:116). The word “Muslim” means one who surrenders to the will of God; no matter what their race, nationality or ethnic background. If a person truly wants to become a Muslim their first step is to speak the “Shahada”, the testimony of faith. In Islam the Shahada...
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...Christianity developed from the Jewish faith in the person and ministry of Jesus of Nazareth. The Christian religion, as with the ancient Greek religion, was first generated through the oral tradition. Jesus was believed to be the only Son of God by the early Christians, and this belief has endured in the modern Christian world. The Holy Bible, consisting of the Old and New Testament, is the basic scripture for Christians, and although Christianity and Greek Mythology are very distinct, they are similar in many interesting ways. Statement of Purpose I am a Christian, and in writing this piece I am in no way saying that Christianity is a myth. It is my intention to point out how Greek mythology and Christianity are similar, and nothing more. It is interesting to learn how ancient people lived and worshiped, and psychology finds mythology useful in classifying modern modes of action. Three Major Ways Greek Mythology and Christianity are Similar Although the gods and heroes of Ancient Greece are not worshiped today, this ancient religion is still comparable to modern Christianity. Greek mythology and Christianity are similar in that they: (1) teach a moral way of life; (2) teach that a woman (Pandora or Eve) caused the downfall of mankind; (3) and both have a savior figure. Although Greek mythology and Christianity are similar, they differ in their concept of sacrifice, punishment, and doctrine, and I will discuss this in a separate piece. A Moral Way of Life To begin, Greek...
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...Lizzie J. Smith Module 1: Assignments Healing Hospital: A Daring Paradigm HLT- 302 Spirituality and Christian Values in Healing Care and Wellness Sunshine Weeks Eric Chapman, founding president and chief executive officer of the Baptist Healing Trust in Nashville, Tennessee, envisioned a healing hospital that would cover not only the physical aspects of healing but the emotional and spiritual components (ericchapman.com, n.d.). My work will describe the healing hospital paradigm and how spirituality influences this paradigm. In addition, barriers to the Healing Hospital paradigm will be discussed along with Biblical scripture that supports compassion in the health care system. The healing hospital paradigm it focuses on the removal of stress and other health risks in the hospital environment for both patients and visitors. . The healing hospital paradigm it involves in healing the whole client (Young & Koopsen, 2006, p. 4) instead of just curing the disease. This emerges from the paradigm’s focuses on healing beyond the body physical: it aims to enhance the overall well being by addressing the patient’s and their families’ cognitive, emotional and spiritual concerns (Milstein, 2005). A healing hospital goes beyond windows, walls, and mortar. Its strong culture of love and caring is what sets it apart from traditional hospitals (Chapman, 2010, p.15)... The concept is to supporting a strong culture of caring for their patients and caregivers. Healing...
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...Here I am on Earth trying to determine if the people here are religious or not?? Here I am on Earth trying to determine if the people here are religious or not. Three things I am looking for are prayer to a higher entity, sacrifice, and public acknowledgment of their god in ways other than prayer. The clues I am now assessing include people attending their houses of worship, people wearing crosses around their necks as jewelry, and people with their heads bowed in prayer. These people with their heads bowed in prayer have so many variations in their actions. Some people are at their dinner table praying, some people kneel on the ground in a line with other people five times per day to pray, and some bow their heads and put their hands together, and use a string of beads to pray with. The earthlings seem to practice sacrifice openly and variably. They are not, perhaps, killing a goat, burning it, and attempting to give it to the deity, but instead, people are sacrificing personal actions. The man who used to steal does not steal anymore, hoping this sacrifice will please his god. So it is with the woman who used foul words heavily in speech. She now, however, is trying to give up (sacrifice) her obscene language because she feels like she cannot reap the benefits of being a servant of her god if her language is not pure. She does not want to grieve her higher being, so she sacrificed her abhorrent language. Sacrifice occurs in Buddhism when practicing Sutra and one...
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...religions. • Identify examples of roles and observances held sacred by indigenous peoples. Week Three: Hinduism • Describe the Hindu philosophical systems and forms of worship. • Explain the origins of Hinduism. Week Four: Buddhism • Explain the origins of Buddhism. • Describe Buddhism’s theological foundations and spiritual practices. Week Five: Daoism, Confucianism, and Judaism • Compare and contrast the theological foundations and spiritual practices of Daoism, Confucianism, Hinduism, and Buddhism. • Explain the origins of Judaism. • Describe Judaism’s theological foundations and spiritual practices. Week Six: Christianity and Islam • Explain the origins of Christianity. • Explain the origins of Islam. Week Seven: Monotheistic Religions • Compare and contrast the theological foundations and spiritual practices of Judaism, Christianity, and Islam. Week Eight: New Religious Movements & Religion in the 21st Century • Compare and contrast new religious movements with well established religions. • Estimate the impact of religious pluralism and the interfaith movement on the future of organized religion. Week Nine: Making Connections • Communicate with people of different religions about their beliefs and practices. Course Materials Fisher, M. P. (2005). Living religions (6th ed.). New Jersey: Prentice-Hall. McInerney, W. (2003). Instructor’s manual with tests: Living religions (5th ed.). New Jersey: Prentice-Hall. All electronic materials...
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