Dean Feeney Experiment 3: Crystallization of proteins.

T = tetragonal lysozyme crystals H = Hexagonal lysozyme crystals
X = Blank well P = precipitate ( M = medium crystals L = large crystals S = small crystals )

Incubated at 20 degrees Celsius | 1 | 2 | 3 | 4 | 5 | 6 | ROW A | T (a few) M | P | X | T (a lot) M | P | X | ROW B | X | P | H (a few) M | X | P | H (a lot) M | ROW C | T (a few) L | P | X | T (a lot) L | P | X | ROW D | X | P | H ( a few) L | X | P | H (a lot) L |

Incubated at 4 degrees Celsius | 1 | 2 | 3 | 4 | 5 | 6 | ROW A | T (a few) S | P | X | T (a lot) S | P | X | ROW B | X | P | H (a few) S | X | P | H (a lot) S | ROW C | T(a few) S | P | X | T (a lot)S | P | X | ROW D | X | P | H (a few) S | X | P | H (a lot) S |

1: The best condition for tetragonal lysozyme crystal formation is Row C in the 5% to 7% NaCL at pH 4.5 (60mg/ml) large crystals
2: The best condition for hexagonal lysozyme crystal formation is Row D in the 2% to 3% NaNO3 at pH 7.5 (60mg/ml) large crystals
3: Tetragonal lysozyme crystals are primarily found in the NaCL solution
4: Hexagonal lysozyme crystals are primarily found in the NaNO3 solution
5: Temperature proved to have a substantial effect on crystal size as all the crystals formed while incubated at 4 degrees Celsius were small crystals.
1HEW Crystal structure.

Experiment 4: Polyacrylamide gel electrophoresis (Analysis of proteins)

M | 1 | 2 | 3 | M | 4 | 5 | 6 |

| 1.

* Impure YFP can be seen in column 1 ranging from 70-10 kDa (binding buffer) * Everything except YFP can be seen in column 2 also ranging from