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Daphnia Magna Heart Rate Experiment

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INTRODUCTION:
The purpose of this investigation was to determine how Daphnia magna heart rate behaves when exposed to increasing concentrations of stimulant and depressant drug.
Hypothesis: If the concentration of caffeine pills and ethanol alcohol are increased then Daphnia magna heart rate will only increase in caffeine pills but not ethanol because caffeine pills are stimulant drug that boost mental and physical function in contrast alcohol is a depressant drug, which reduces the levels of arousal in the central nervous system (Ceida, n.d.).
Independent Variables: 0%, 3%, 5% ethanol concentration.
0%, 3%, 5% caffeine pill concentration.
Dependent Variable: heart rate of Daphnia magna/beats per minute (bpm).
Constant Variables: Daphnia magna, caffeine pills, ethyl alcohol, 1 inch masking tape, 100ml volumetric flask, 100ml beakers, 10ml graduated cylinder, containers, light microscope, wash bottle, coffee filter, mortar and pestle, distilled water, and concavity slides.
REVIEW OF LITERATURE:
Caffeine and alcohol plays a vital role in every daily life of humanity, but which concentrations greatly affect the heart rate of Daphnia magna? So, what are Daphnia magna …show more content…
On the contrary, 1% concentration of caffeine had also little effect on Daphnia magna after 40 minutes submergence increase above 100% heart rate and eventually recovered after 1 hour and 10min (70min). Although increasing concentrations of 0.5% and 2% had decreased the heart at 50 minutes continuously decreasing bit by bit throughout the experiment which mingles around 80% to 70% heart rate. The reason of the sudden decrease, because the concentrations 0.5% and 2% at 10 minutes was at first a little above 100% heart rate that conveys the Daphnia magna was pumping really hard hence the trend leads that it evidently exhausted the heart rate of Daphnia magna from 20 minutes throughout the

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