...The Daphnia magna is a small crustacean that was used for the experiment. The purpose of this lab experiment was to determine how both caffeine and alcohol would affect the heart rate of a Daphnia magna. The results showed that when caffeine was added to the Daphnia magna, its heart rate increased faster than when alcohol was added. In this lab report, the steps are explained on how we conducted the experiment. Also, any errors that happened during the experiment will be analyzed more in detailed in this lab report. Introduction Daphnia magna is a little crustacean that is found living in ponds. It duplicates by sexual reproduction, varying with the creation of asexual offspring (Roulin, 2013). The Daphnia magna is a transparent crustacean...
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...Daphnia magna is a transparent water flea species found in rocky pools along the Atlantic Ocean. The Daphnia’s body is encased in a clear structure called carapace (Elenbaas, 2013). The exoskeleton, carapace, provides protection for the Daphnia magna, “The portion of the hard exoskeleton, or shell, that covers all or part of the body of many crustaceans…” (“Definition, n.d.). As John Clare says, “Generally the life span increases as temperature decreases, due to lowered metabolic activity” (2002). Daphnia magna are able to sustain life approximately 16 days longer at 20 Celsius when compared to a life cycle at 25 Celsius (Clare, 2002). The heart can be easily seen under a light microscope due to the transparency of the skin (Handy, 2012). A...
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...Daphnia Magna are small crustaceans that are excellent for observing the effect of depressants on their nervous system. Daphnia may be used in certain environments to test the toxicity levels in an ecosystem. Due to the easy visibility of the heart, daphnia are mostly used to monitor the effects of certain drugs. Scientists can thoroughly investigate the progression of chemicals on their metabolic processes. The purpose of this specific experiment is to observe the effect of ethanol, aspirin, and caffeine on daphnia’s heart rate. In the past, these compounds have not been able to provide sufficient results, however daphnia might be able to provide consistent results. Not only do these chemicals affect heart rate, but they also affect frequency of reproduction, number of eggs produced, and body structure. Pesticides similar to these chemicals can be released into the environment which is why daphnia might be useful in the task of monitoring toxicity levels. They are also invaluable to...
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...Daphnia shows a lot of similarities to higher crustaceans in their entire morphology, nervous system (Chen et .al 2005). The first antennae are considered to be the main chemoreceptor organ of Daphnia which are sexually dimorphic, long and having an additional flagellum in males but remaining close to the body in females. For example: in Daphnia magna, the first antennae of males are approximately 400 microns long while in females they are about 100 microns long. In Daphnia pulex the antennule length is250um in male and in females remains close to the body but setae length being similar to males being 30 microns long. Daphnia magna have more pronounced first antennules in females and in males the flagellum is heavily ciliated. Understanding...
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...the caffeine concentration rises, so does the mean heart rate of daphnia. Risk Assessment: In this experiment there are the following risks both for the human that carries out the experiment and the daphnia: ← The human that carries out the experiment should be cautious not to touch the lamp with wet hands since it uses electricity. ← Also, be careful not to spill any of the pond water on the floor. ← As for the daphnia, there is a chance for them to die as when they are exposed to light, they are also exposed to heat in which they aren’t used to. ← Moreover, when they are given caffeine of a high concentration their heart may beat so fast that will cause their death. ← Furthermore, there is the risk of the daphnia to dry up, which may kill it in the middle of the experiment. Method: These are the steps that we followed when doing the experiment: 1. Use a pipette to transfer the daphnia to a cavity slide containing pond water of known volume. 2. Add some cotton fibres to the cavity slide and move them with a fine paint brush in such a way that the daphnia can’t move. 3. Use a calculator to calculate the heart rate. Set its reading to zero and add 1 to the answer. 4. One student will use a stopwatch to count 30 seconds which is the time during which the measurement of the heart rate will take place. 5. Turn the microscope lamp on and locate the heart of the daphnia through the microscope. 6. Measure the beat rate. 7. Repeat...
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...9-21-2014 Bio 102 The Outcome of Temperature and Epinephrine on the Heart rate of Daphnia INTRODUCTION This lab report accomplishes a test to measure the effects of an escalation and a reduction in temperature, and amount of Epinephrine on the heart rate of Daphnia. The Daphnia also known as ´´water flea´´ is a type of crustaceans that is found lavishly in small bodies of fresh water. Only a few of these are impartially big enough to be seen without a microscope in decent light. Daphnia have a tendency to be virtually kidney shaped, retaining only a single compound eye, two doubly separated antennae , and leaf –like limbs inside the carapace that harvest a contemporary of water which transports food and oxygen to the mouth and gills. It’s fascinating how their bodies are virtually transparent and through a microscope the heart beating can be seen, and occasionally their last meal can be seen through the microscope as well. HYPOTHESIS I hypothesized that as the temperature is increased the Daphnia heart rate will increase; lower temperature will cause the heart rate to slow down. I also hypothesized that the amount of Epinephrine will eventually kill the Daphnia. MATERIALS AND METHODS In order for our experiment to be successful there were materials we used and methods. Our professor gave each group one Daphnia, afterward we used a clean glass microscope slide and dried it with a paper towel. Then we placed the slide on the stage of the microscope...
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...Daphnia Introduction: Daphnia is a large genus comprising over 200 species of small freshwater crustaceans that belongs to the family Daphniidae and the order “Cladocera”. The common name of Daphnia is water flea and this name is due to jerky-flea like swimming movement. Daphnia often live is freshwater bodies of relative different quality including swamps, lakes, streams and rivers. Description: Daphnia in general are small and may range from as small as 0.5 mm to as long as 5.0 mm in length, sometimes more; and hence some daphnia species can be seen by naked eyes (e.g. Daphnia magna) , while others must are microscopic (e.g. Daphnia pulex). Usually females are larger than males and have a brood chamber under their carapace where eggs are kept. The uncalcified...
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...Materials: • Scales • Watch Glass • Pipette • 1x 125ml beaker • 1x 50ml measuring cylinder • 1x spoon • 1x mortar and pestle • 1x no-doz tablet • 150ml pond water • Daphnia • Microscope slides • Microscopes • Journal/Pen • Marker • Scissors • 50ml Beaker • Plastic cup • timer Method: 1. Collect all materials stated in the materials section above and draw up a table in your journal to record your data later on. 2. Pour 50mls of pond water using the measuring cylinder into the 125ml beaker. (if temperature is to cold change from a beaker to a plastic cup as the daphnia are quite sensative.) 3. Using a Marker, mark on the beaker what drink you are using and its caffeine concentration. 4. Take one no-doz tablet and using the Mortar and...
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...This experiment is to show how the heart rate can be affected by the substances caffeine and ethanol,Daphnia (Daphnia magna) are prone to alcohol and caffeine intoxication and react quickly to both solutions, therefor the effect of both the depressant and stimulant on the heart rate can be seen through the translucent exoskeleton . (John K. Colbourne) Caffein (trimethylzanthine) is a psychoactive drug, it is found in large quantities in a array of different foods and drinks.It is a cardiac stimulant, this occurs by it being a anti catalyst to the enzyme cyclic phosphodiesterace (Neal J. 1999) which keeps the heart at a steady pace with the help of C.A.M.P (cyclic adenosine monoposphate) this is produced by the hormone epinephrine. Without cyclic phosphodiesterace the heart beat increases, this is because the C.A.M.P diffuses through cells and activates...
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...were both increased and decreased with the addition of ethanol depending on the level of stress the rats were in (Arciero 1998). In humans, caffeine did not affect heart rates (Sparrow 1987). Different ages of humans had heart rates that were not affected by caffeine (Umemura 2006). The purpose of this experiment was to test if the quantity of ethanol and caffeine increases or decreases the heart rates in Daphnia magna. We predicted that the heart rates of the Daphnia would rise when exposed to ethanol, yet would remain the same when exposed to caffeine. We also hypothesized that the higher the concentration of the drug in the solution, the more effect the drug would have on the organism. Methods We conducted the experiment on Daphnia...
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...Discussion: The purpose of this experiment was to determine how various drugs will affect basal heart rate of daphnia. This is relevant because due to Daphnia’s similar cardiac tissues to humans (Woodham et al., 2017), it can be seen how various drugs will affect human cardiac tissue. Acetylcholine and Lidocaine have been hypothesized to decrease heart rate in Daphnia. Epinephrine and Caffeine are proposed to increase heart rate. In regards to Acetylcholine and Lidocaine, it was hypothesized that Acetylcholine and Lidocaine should decrease Daphnia heart rate, and as demonstrated by the original experimental results (Figure 2), Daphnia heart rate in the presence of Acetylcholine and Lidocaine both decreased. This is also supported when compared...
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...Daphnia Practical Write-up Purpose and Hypothesis The purpose of this experiment was to determine how affected the heart rate of the daphnia. The results gained from this experiment aids scientific researchers in finding out how caffeine can affect heart rate as well as human health. My hypothesis was that as we increase the caffeine dose the daphnia heartbeat would increase at the same time. Variables The independent variable was the amount of caffeine we administrated to the daphnia. The dependent variable was the heartbeat of the daphnia before and after their caffeine intake. The control variables were the species of daphnia used, the water temperature and the type of microscope used Method Ultimately our method was successful despite the experiment itself not being successful .We was able to carry out all of the steps without difficulty despite environmental troubles. 1. Gather all the relevant equipment including the daphnia, solutions of no caffeine concentration and two of different solutions of caffeine concentrations. 2. Remove the daphnia from its initial environment using a pipette, disposing of excess water in a few drops. 3. Pipette the daphnia onto a microscope side into the dipped part, remove excess water still remaining using a filter paper or a paper towel. 4. Add a few drops of distilled water and remove excess water using a filter paper or a paper towel to prevent the daphnia from moving around more than need. 5. Focus the daphnia in the...
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...based on those observations, then perform the fix/experiment, then record the results. In lab number two, Daphnia organisms will be placed in water, a caffeine solution, and an alcohol solution, and for each solution the Daphnia is placed in, the organism will be observed and the heartbeat will be recorded. Making these observations will allow an insight to how the organism and other organism are affected by both caffeine and alcohol. Like humans, will the Daphnia be sluggish after being exposed to alcohol and...
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...sources for metabolic efficiency. It is generally known that the metabolic rate, and thus the heartrate, of animals scale exponentially with a change in temperature. A common rule of thumb is that the metabolic rate of an animal doubles with a 10 ͦC increase in temperature (Lawson, 2001) Daphnia, more commonly known as water lice, are soft-bodied aquatic animals used for live feeding of aquarium fish. The transparent exoskeleton of this animal makes it...
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...drops of caffeine to six Daphnis for each temperature of the five different temperatures we are testing, 15°C, 20°C, 30°C, 35°C, and 40°C. after adding the caffeine, we calculated the BPM for each Daphnia individually, then the average for each temperature was taken. The independent variables that we manipulated in this experiment were the amount of the caffeine that was added to the Daphnia’s and the temperature of the water. The dependent variable we measured was the heartbeat of Daphnia’s per minute. One of the confounding variables was the concentration of the water in which the Daphnia’s was in. however; the same environment was used for all the Daphnia’s, which eliminates the effect of this variable in the result. The same light source and the same room temperature (baseline) were used in the all measurement of the...
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