9-21-2014
Bio 102 The Outcome of Temperature and Epinephrine on the Heart rate of Daphnia INTRODUCTION
This lab report accomplishes a test to measure the effects of an escalation and a reduction in temperature, and amount of Epinephrine on the heart rate of Daphnia. The Daphnia also known as ´´water flea´´ is a type of crustaceans that is found lavishly in small bodies of fresh water. Only a few of these are impartially big enough to be seen without a microscope in decent light. Daphnia have a tendency to be virtually kidney shaped, retaining only a single compound eye, two doubly separated antennae , and leaf –like limbs inside the carapace that harvest a contemporary of water which transports food and oxygen to the mouth and gills. It’s fascinating how their bodies are virtually transparent and through a microscope the heart beating can be seen, and occasionally their last meal can be seen through the microscope as well. HYPOTHESIS I hypothesized that as the temperature is increased the Daphnia heart rate will increase; lower temperature will cause the heart rate to slow down. I also hypothesized that the amount of Epinephrine will eventually kill the Daphnia. MATERIALS AND METHODS In order for our experiment to be successful there were materials we used and methods. Our professor gave each group one Daphnia, afterward we used a clean glass microscope slide and dried it with a paper towel. Then we placed the slide on the stage of the microscope, it was important to close the iris diaphragm in order to reduce the quantity of light so we can see the daphnia. We then concentrated with the scanning lens and found the heart. After it was focused, we used a kimwipe to remove some of the water, its important no to scar up the Daphnia in the course. We left just the right amount of water consequently that the Daphnia is lying in a small ´´puddle´´; if the gills are dried out the Daphnia will die. After we found the heart, we used a tally counter and made three 15 second count of the heartbeat. The results for this trail are attached in my report. Next we poured just about 50ml of water from the flask in the 53 C incubator into a trivial beaker. We added a drop of water from the beaker to our Daphnia on the slide. We left the water on the slide for 30 seconds; we blotted some of the liquid and added another drop of the 53C water. We repeated this step for about five times. It’s important to have a sense of urgency during this step because we wanted to guarantee that the temperature reminded at 53C. The results for this part are also attached in my lab report. The next step was using the ice cubes, we used the same Daphnia because this lets us know how the heart is effected whether we used room temperature, warm water or ice cubes. We took the slide with the Daphnia and placed it on top of the ice for about three minutes. When the temperature of the Daphnia has dropped to zero degrees, we positioned the petro dish with the slide and our Daphnia on the microscope stage. Once we found the heart, we started to count 15 seconds using the tally counter and recorded our data. Before we moved on to the final step, it was mandatory for the Daphnia to return to room temperature. This was important to get the Daphnia to room temperature, in order to know that there were no other factors interfering with the drop of Epinephrine. Once it was in room temperature we counted 15 seconds and recorded its heartbeat before the Epinephrine. Next we added two drops of the epinephrine (adrenalin) solution to the water drop and delayed five minutes. We tarnished up any extra fluid and too three 15 second counts of the heart rate and recorded our data. Once this step was completed logically the next would have been to return the Daphnia in its marked container, nevertheless our Daphnia died after we added the epinephrine. RESULTS
After this experimentation one of the hypothesize I made was supported. I stated in my paper that as the temperature increased the heart rate of Daphnia will also increase, and when the water was at room temperature the heart will be slower. I observed the heart beating fast when the water was at 53C compared to when it was at room temperature. The interesting result was using the Epinephrine because our Daphnia died. It was remarkable because out of the whole class only my groups Daphnia died after the epinephrine was added. Since our Daphnia died, it would have been great to use more than one daphnia because they might have reacted to the epinephrine differently. Since Daphnia is an ectothermic (cold blooded animal) which is basically an organism that cannot preserve its body temperature, so its independent on the environment. Therefore when the temperature drops, the heart rate moves slower, and reactions occur slower. Over all this was a very informative experiment, I did know a few things about Daphnia before this experiment; nevertheless it was great using the microscope to see its heart rate.