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Dna Extraction

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Submitted By egarzon91
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LAB#11

DNA EXTRACTION & KARYOTYPING

________________________________________________
Objectives:

After completing this exercise, you should be able to:

1. Develop an understanding of the structure and properties of DNA, based on observation and manipulations 2. Understand some implications of DNA technology 3. Extract DNA from an onion to understand that all cells contain DNA

Introduction/Purpose:

DNA is too small to see under a regular microscope, so then how can it be studied? DNA is a large chemical molecule found in all living things, so it should be possible to extract it from cells or tissue. All we need to do is disrupt the cell’s plasma membrane and nuclear envelope, make the DNA clump together. DNA extraction is possible. Plant material is easy to use and DNA extractions from onion, bananas, liver, or wheat germ are common classroom activities or demonstrations. Plants used in agriculture and horticulture are often artificially selected for their large flowers and fruits. Strawberries are no exception. A reason for the size of today’s large supermarket strawberries is the octaploid nature of their cells. With eight sets of chromosomes, they have plenty of DNA for classroom extraction.

The fruit is homogenized with a detergent to prepare a filtrate. The detergent emulsifies and forms complexes with the lipids and proteins of the plasma membrane this causes them to precipitate out of the solution. The mixture is then filtered through cheesecloth; the filtrate contains the DNA and the proteins.

Materials/Equipment:

Ice cold blender, Ice cold homogenizing medium, Ice cold 95% ethanol, Ice cold 100 ml graduated cylinder, Ice cold plastic test tube, Ice cold beaker, cheesecloth, 2 strawberries, test tubes, glass stirring rods, ice bath.

Procedure:

1. Everything has to be cold! 2. Pour 100 ml of Homogenizing…...

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