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Egg Windowing

In: Science

Submitted By reyesshanee
Words 750
Pages 3
June Hayden R. Sinson

Ramon Magsaysay High School - Espaǹa

Arturo A. Tolentino,

Caloocan City Science High School

 The

study of development has been greatly aided by the use of the chick embryo as an experimental model.

It is necessary to open the eggshell and reclose it without perturbing the embryo's growth. The embryo can be examined at successive developmental stages by re-opening the eggshell. 

Protocol

Maintain

at 37°C with relative humidity that was set above
60%, remove the eggs from the incubator.  Turn

eggs 90 degrees so that the large base lies horizontal where your embryo is located. Saturate

a stack of nonsterile gauze with 70% ethanol. Use

two to three pieces to swab up to 5 eggs.
Discard when the gauze is soiled.

 Cut

and place a 1" x 1" piece of plastic tape just left of the base to protect the area where the albumen will be drawn out.  Use

the point of a pair of scissors to make a small hole in the middle of the tape.  Using

a 10 cc syringe with an 18gauge, 1-inch needle, slowly drill the needle through the hole made by the scissors.  Drive

the needle down at a 45°C angle towards the bottom of the egg.
 Tilt the needle towards the center and draw up 3 to 4 mL of albumen.

 Cut

a 3" x 3" piece of plastic tape and stretch it to fit on the top of the egg.
Extend the corners of the square around the rounded ends of the horizontal surface of the eggs, being careful not to pull too hard.
Pull the tape so that it is tight against the surface of the eggs with no folds.

 Using

a pair of sharpstraight 4" dissection scissors, twist a hole into the bottom center of the area where the tape was placed.  Slowly

guide the lower blade of the scissors into the egg being sure to keep the tips

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