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Enzyme Activity in Living Tissue Lab

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Submitted By 19DieckmanJ
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Enzyme Activity In Living Tissue Lab
Jaida Dieckman
November 23, 2015

Background The studies done during this lab were the enzymes and there role is having proteins that are used as catalysts in living things. Also, substrates are reactants that connect to and enzymes active site. The role of the enzymes catalase in a cell is it breaks down the substrate hydrogen peroxide. Hydrogen peroxide is produced during normal metallic activities in the cell, but it is poisonous to the cell if it accumulates. Eventually, hydrogen peroxide will break down into water and oxygen. The accumulation of the poison in the cell would damage the cell long before it breaks down naturally. The cell naturally produces the enzyme catalase to react with the hydrogen peroxide and break it down as rapidly as it is produced to avoid destruction of the cell. The purpose of this lab was to analyze the enzymes in living tissues, represented by the pieces of liver. Also, hydrogen peroxide was used to demonstrate these effects.
Procedure
In this lab was tested five different substances. In part A the purpose was to observe untreated normal catalase reaction by using small tweezers to obtain two pieces of liver and one piece of each of the other tissues. Using a small beaker 30ml of hydrogen peroxide was obtained. A graduated cylinder was used to measure 3ml of hydrogen peroxide into 5 different test tubes. Then used an expo marker to mark the top of the hydrogen peroxide. A piece of liver was put into the first test tube containing hydrogen peroxide. In part B the purpose was to figure out which tissues contained catalase. 3 ml of hydrogen peroxide was places in 4 additional test tubes. Then one piece of each of the 4 tissues were put into the hydrogen peroxide in separate test tubes. A second line was drawn at the point where the bubbles stopped. Treatment A under “untreated” was the

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