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Enzymes

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Enzymes
Ally Wormaan
11/07/2013
Section 018
Introduction:
Our body has many cells, cells which operate like a chemical factory. Chemicals are broken down for things like energy, and new chemicals are then synthesized. The food we eat only supplies us with some of the compounds that are needed for our body to operate. Most that are needed are synthesized within the cell by hundreds of different types of reactions that are all part of metabolism. All of these reactions wouldn’t be able to take place at body temperature. Or rather if they did, they would do so at a very slow rate. Enzymes are what allow the reactions to take place rapidly and efficiently. In this lab we will be demonstrating the role of enzymes as described, and also observing how the different concentration, temperature, pH, and inhibitors will affect the enzyme activity.
Procedure:
Prior to starting we will need to have three water baths readily available. One will be at 0-5*C, second at 35-40*c, and the last at boiling. The first part of this lab has us working with catalysis. We take three test tubes and add 2mL of 3%H2O2 to each. Test tube one will receive a small piece of raw liver. Test tube two receives a small piece of cooked liver, and test tube three does not receive anything. We will observe which solution quickly produces foam, and record our observations. We move onto enzyme concentrations. Again taking three test tubes we fill them as follows: Test tube # | 2% Rennin Solution mL | Distilled water mL | Milk,Ml | 1 | 4 | 0 | 3 | 2 | 2 | 2 | 3 | 3 | 1 | 3 | 3 |
Then we add 1mL of 3M HCL to each test tube and mix. At the same time we will place all three test tubes into the second water bath, 35-40*c. Using a stop watch we will keep track of the time the test tubes are in the water bath. We need to record the order in which the solutions form a precipitate. Now we move

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